Soluble anti mous cd28

Manufactured by BD

Soluble anti-mouse CD28 is a laboratory reagent used in immunological research. It is a recombinant protein that binds to the CD28 receptor on mouse T cells, providing a costimulatory signal that can activate and stimulate T cell proliferation and cytokine production. This product can be used in cell culture experiments to study T cell activation and function.

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Lab products found in correlation

Retinal, by Merck Group (4 mentions) Anti mouse cd4 microbeads, by Miltenyi Biotec (4 mentions) Human recombinant transforming growth factor β tgf β, by Thermo Fisher Scientific (4 mentions) Anti human cd4 microbeads, by Miltenyi Biotec (4 mentions) Anti mouse cd3, by BD (4 mentions) Anti il 4, by Thermo Fisher Scientific (4 mentions) Anti ifn γ, by BD (4 mentions)

4 protocols using soluble anti mous cd28

Isolation and Induction of Regulatory T Cells

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Mouse splenic CD4 + T cell were isolated from spleen by using mouse anti-CD4 microbeads (Miltenyi Biotec, Germany). To isolate Treg cells, CD4 + T-cells were cultured with plate-bound anti-mouse CD3 (1 μg/mL; BD Biosciences), soluble anti-mous CD28 (1 μg/mL; BD Biosciences), anti-IFN-γ (2 μg/ml), anti-IL-4 (2 μg/ml), human recombinant transforming growth factor-β (TGF-β; 5 ng/mL; PeproTech, London, UK), and Retinal (0.1 μM; Sigma-Aldrich, St. Louis, MO)(16).
Human CD4 + T cells were isolated from PBMC by using human anti-CD4 microbeads (Miltenyi Biotec, Germany). To isolate human Treg cells, human CD4 + T-cells were cultured with plate-bound anti-human CD3 (1 μg/mL), soluble anti-human CD28 (1 μg/mL), anti-IFN-γ (2 μg/ml), anti-IL-4 (2 μg/ml), human recombinant TGF-β (5 ng/mL) and Retinal (0.1 μM).
After three days, the induced Treg cells were stained with CD4, CD25 and then sorted using a MoFlo cell sorter to obtain a ~ 95% pure CD4 + CD25 + population.

Park M.J., Baek J.A., Kim S.Y., Jung K.A., Choi J.W., Park S.H., Kwok S, & Cho M.L. (2020). Myeloid-derived suppressor cells therapy enhance immunoregulatory properties in acute graft versus host disease with combination of regulatory T cells. Journal of Translational Medicine, 18, 483.

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Isolation and Induction of Murine and Human Tregs

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Mouse splenic CD4+T cell were isolated from spleen by using mouse anti-CD4 microbeads (Miltenyi Biotec, Germany). To isolate Treg cells, CD4+ T-cells were cultured with plate-bound anti-mouse CD3 (1 μg/mL; BD Biosciences), soluble anti-mous CD28 (1 μg/mL; BD Biosciences), anti-IFN-g (2 mg/ml), anti-IL-4 (2 mg/ml), human recombinant transforming growth factor-β (TGF-β; 5 ng/mL; PeproTech, London, UK), and Retinal (0.1 μM; Sigma-Aldrich, St. Louis, MO)(16).
Human CD4+T cells were isolated from PBMC by using human anti-CD4 microbeads (Miltenyi Biotec, Germany). To isolate human Treg cells, human CD4+T-cells were cultured with plate-bound anti-human CD3 (1 μg/mL), soluble anti-human CD28 (1 μg/mL), anti-IFN-g (2 mg/ml), anti-IL-4 (2 mg/ml), human recombinant TGF-β (5 ng/mL) and Retinal (0.1 μM).
After three days, the induced Treg cells were stained with CD4, CD25 and then sorted using a MoFlo cell sorter to obtain a ~95% pure CD4+CD25+ population.

Park M., Baek J., Kim S.Y., Jung K., Choi J.W., Park S., Kwok S., & Cho M. (2020). Myeloid-derived suppressor cells therapy enhance immunoregulatory properties in acute graft versus host diseas with combination of regulatory T cells.

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Isolation and Expansion of Regulatory T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse splenic CD4 + T cell were isolated from spleen by using mouse anti-CD4 microbeads (Miltenyi Biotec, Germany). To isolate Treg cells, CD4 + T-cells were cultured with plate-bound anti-mouse CD3 (1 µg/mL; BD Biosciences), soluble anti-mous CD28 (1 µg/mL; BD Biosciences), anti-IFN-γ (2 µg/ml), anti-IL-4 (2 µg/ml), human recombinant transforming growth factor-β (TGF-β; 5 ng/mL; PeproTech, London, UK), and Retinal (0.1 µM; Sigma-Aldrich, St. Louis, MO)(16).
Human CD4 + T cells were isolated from PBMC by using human anti-CD4 microbeads (Miltenyi Biotec, Germany). To isolate human Treg cells, human CD4 + T-cells were cultured with plate-bound anti-human CD3 (1 µg/mL), soluble anti-human CD28 (1 µg/mL), anti-IFN-γ (2 µg/ml), anti-IL-4 (2 µg/ml), human recombinant TGF-β (5 ng/mL) and Retinal (0.1 µM).
After three days, the induced Treg cells were stained with CD4, CD25 and then sorted using a MoFlo cell sorter to obtain a ~ 95% pure CD4 + CD25 + population.

Park M., Baek J., Kim S.Y., Jung K., Choi J.W., Park S., Kwok S., & Cho M. (2020). Myeloid-Derived Suppressor Cells Therapy Enhance Immunoregulatory Properties in Acute Graft Versus Host Diseas with Combination of Regulatory T Cells.

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Isolation and Induction of Murine and Human Tregs

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse splenic CD4+T cell were isolated from spleen by using mouse anti-CD4 microbeads (Miltenyi Biotec, Germany). To isolate Treg cells, CD4+ T-cells were cultured with plate-bound anti-mouse CD3 (1 μg/mL; BD Biosciences), soluble anti-mous CD28 (1 μg/mL; BD Biosciences), anti-IFN-g (2 mg/ml), anti-IL-4 (2 mg/ml), human recombinant transforming growth factor-β (TGF-β; 5 ng/mL; PeproTech, London, UK), and Retinal (0.1 μM; Sigma-Aldrich, St. Louis, MO)(16).
Human CD4+T cells were isolated from PBMC by using human anti-CD4 microbeads (Miltenyi Biotec, Germany). To isolate human Treg cells, human CD4+T-cells were cultured with plate-bound anti-human CD3 (1 μg/mL), soluble anti-human CD28 (1 μg/mL), anti-IFN-g (2 mg/ml), anti-IL-4 (2 mg/ml), human recombinant TGF-β (5 ng/mL) and Retinal (0.1 μM).
After three days, the induced Treg cells were stained with CD4, CD25 and then sorted using a MoFlo cell sorter to obtain a ~95% pure CD4+CD25+ population.

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