Transmission electron microscopy (TEM) images were acquired at 80 keV with either a Tecnai T12 microscope (FEI, Hillsboro, OR) equipped with a bottom mounted US1000 2 K × 2 K CCD camera with a 14 µm pixel size (Gatan Inc, Pleasanton, CA), or a JEM 1010 microscope (JEOL, Tokyo, Japan) with a side entry 1 K × 1 K video rate camera with a 7.2 µm pixel size (Hammamatsu Photonics, Hammamatsu, Japan). Scanning electron microscopy (SEM) images were acquired with a Quanta 250 (FEI, Hillsboro, OR) at near atmospheric pressure using a gaseous secondary electron detector. Average nanoparticle diameters were measured manually in ImageJ software (NIH, version Java 1.6.0_20 64-bit) for 50–100 nanoparticles from TEM images at various magnifications. UV-Vis spectra were recorded on an Evolution 201 spectrometer (Thermo Scientific, Waltham, MA). A FEI Quanta 600 FEG ESEM equipped with an Octane Silicon Drift detector (EDAX, Inc.) was used for the energy dispersive x-ray spectroscopy (EDS) analysis (using TEAM EDS software). Gold concentrations were determined using ICP-OES.
Us1000 2 k 2 k ccd camera
Manufactured by Ametek
The US1000 2 K × 2 K CCD camera is a high-resolution image sensor that captures digital images with a resolution of 2048 × 2048 pixels. It utilizes a charge-coupled device (CCD) technology to convert light into electrical signals, which are then processed and stored as digital image data.
Automatically generated - may contain errors
Lab products found in correlation
Vitrobot mark 4, by Thermo Fisher Scientific (2 mentions)
Jem 1010 microscope, by JEOL (1 mentions)
Quanta 250, by Thermo Fisher Scientific (1 mentions)
Tecnai t12 microscope, by Thermo Fisher Scientific (1 mentions)
Octane silicon drift detector, by Ametek (1 mentions)
Evolution 201 spectrometer, by Thermo Fisher Scientific (1 mentions)
Cf300 cu ul, by Electron Microscopy Sciences (1 mentions)
Ultracut s ultramicrotome, by Leica (1 mentions)
Eponate 12 resin, by Ted Pella (1 mentions)
Jem 1400 tem, by JEOL (1 mentions)
Tecnai g2 spirit microscope, by Ametek (1 mentions)
4 protocols using us1000 2 k 2 k ccd camera
1
Multimodal Characterization of Nanoparticles
2
Negative Staining for TEM Imaging
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Square mesh thin carbon-coated copper EM grids (CF300-CU-UL, Electron Microscopy Sciences) were prepared for negative stain EM images. Uranyl formate (UF) was prepared as a 1% w/v solution in deionized water and protected from light. The solution was filtered at 0.02 μm before use. Samples were diluted in 10 mM HEPES pH 7.5, 150 mM NaCl to 0.125 mg mL−1 (protein and lipids each). An aliquot of 4 μL of sample was added to a glow discharged grid, positioned over ice. The sample was then blotted for 1 or 2 min with Whatman filter paper. The blotted grid was then touched to a 20 μL well of UF for 30 s, then blotted with filter paper, followed by an additional UF touching/blotting step for 1 min. Drying of the grid was initiated with a slow stream of N2 gas for approximately 30 s, then the grids were left overnight to dry completely on filter paper in a covered petri dish before storing. Negative stain EM images were collected on a FEI Technai TF200 microscope operated at 200 kV. Images were collected at 1700 and ×6500 magnification using a Gatan US1000 2k × 2k CCD camera.
3
Ultrastructural Analysis of V2O5 Exposure
4
Transmission Electron Microscopy of Nanoparticles
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TEM was performed
on a FEI Tecnai G2 Spirit microscope equipped with a Gatan US1000
2k × 2k CCD camera and LaB6 cathode operated at 120
kV. Images were recorded using freshly glow discharged carbon coated
copper grids (CF300-Cu, 300 mesh). For nonstained samples, 5 μL
of nanoparticle solution (β = 50 mg·L–1 in Milli-Q water) was drop-coated on the TEM grid surface and removed
with a filter paper after 1 min. For negatively stained samples, 5
μL of nanoparticle solution (β = 50 mg·L–1 in Milli-Q water) was drop-coated on the TEM grid and removed with
a filter paper after 1 min. Next, 5 μL of uranyl acetate solution
(2 wt % in ethanol) was added and removed after 15 s incubation time.
All sample-deposited grids were air-dried overnight before measurement.
Software ImageJ 1.52h (National Institutes of Health, U.S.A.) was
used for image evaluation.
For cryogenic TEM (cryo-EM) sample
preparation, 3 μL of the nanoparticle solution (5 mg/mL, in
Milli-Q water) were applied to freshly glow-discharged carbon grids
with a copper 200 mesh (Quantifoil Micro Tools GmbH). Grids were plunge-frozen
in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific)
set at 22 °C, 100% humidity, and 2.5 s blotting time. Samples
were imaged in a Talos L120C transmission electron microscope equipped
with a LaB6 filament operating at a 120 kV accelerating voltage. Images
were recorded using a Ceta camera.
on a FEI Tecnai G2 Spirit microscope equipped with a Gatan US1000
2k × 2k CCD camera and LaB6 cathode operated at 120
kV. Images were recorded using freshly glow discharged carbon coated
copper grids (CF300-Cu, 300 mesh). For nonstained samples, 5 μL
of nanoparticle solution (β = 50 mg·L–1 in Milli-Q water) was drop-coated on the TEM grid surface and removed
with a filter paper after 1 min. For negatively stained samples, 5
μL of nanoparticle solution (β = 50 mg·L–1 in Milli-Q water) was drop-coated on the TEM grid and removed with
a filter paper after 1 min. Next, 5 μL of uranyl acetate solution
(2 wt % in ethanol) was added and removed after 15 s incubation time.
All sample-deposited grids were air-dried overnight before measurement.
Software ImageJ 1.52h (National Institutes of Health, U.S.A.) was
used for image evaluation.
For cryogenic TEM (cryo-EM) sample
preparation, 3 μL of the nanoparticle solution (5 mg/mL, in
Milli-Q water) were applied to freshly glow-discharged carbon grids
with a copper 200 mesh (Quantifoil Micro Tools GmbH). Grids were plunge-frozen
in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific)
set at 22 °C, 100% humidity, and 2.5 s blotting time. Samples
were imaged in a Talos L120C transmission electron microscope equipped
with a LaB6 filament operating at a 120 kV accelerating voltage. Images
were recorded using a Ceta camera.
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