Silencer select validated hif 1α sirna

Cells were transfected with the corresponding small interfering RNA (siRNA) using Lipofectamine™ RNAiMAX lipid reagent (Thermo Scientific) as per manufacturer's instructions and previously described^{5 (link),56 (link)}. Briefly, 1 × 10⁵ cells/cm² hPSCs were plated unicellular on Vitronectin-coated MWx24 cell culture dishes, grown 24 h with E8 media, and then transfected with Silencer Select Negative Control #2 (Ambion™, Cat. #4,390,846) or Silencer Select Validated HIF-1α siRNA (Ambion™, Cat. #4,390,824, siRNA ID: s6539) or Silencer Validated P53 siRNA (Ambion™, Cat. # AM51331, siRNA ID: 106,141). The concentration of siRNA used for cell transfection was 20 nM for HIF-1α siRNA and 50 nM for P53 siRNA.

Cells were transfected with the corresponding small interfering RNA (siRNA) using Lipofectamine RNAiMAX lipid reagent (INVITROGEN) as per manufacturer's instructions and as previously described^{18 (link),53 (link)}. Briefly, 2 × 10⁵ cells were plated unicellular on Vitronectin-coated 24-well dishes, grown 24 h with E8 media and then transfected with Silencer Select Negative Control #2 (AMBION, Cat. #4390846) or Silencer Select Validated HIF-1α siRNA (AMBION, Cat. #4390824, siRNA ID: s6539) or Silencer Select Validated EPAS1 (HIF-2α) siRNA (AMBION, Cat. #4390824, siRNA ID: s4699) or Silencer Select Validated BNIP-3 siRNA (AMBION, Cat. #4392420, siRNA ID: s2059) or Silencer Select PMAIP1 (NOXA) siRNA (AMBION, Cat. #4392420, siRNA ID: s10709) or Silencer Validated P53 siRNA (AMBION, Cat. # AM51331, siRNA ID: 106141). The concentration of siRNA used for cell transfection was 20 nM.