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P42 44 mapk erk1 2

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P42/44 MAPK (ERK1/2) is a protein that plays a key role in the mitogen-activated protein kinase (MAPK) signaling pathway. It is involved in the regulation of cell growth, differentiation, and survival.

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Lab products found in correlation

Image lab software, by Bio-Rad (2 mentions) P38 mapk, by Cell Signaling Technology (2 mentions) Ecl substrate, by Bio-Rad (2 mentions) Chemidoc, by Bio-Rad (2 mentions) Shp 1, by Cell Signaling Technology (2 mentions) Af 400, by Cell Signaling Technology (2 mentions) Myosin 9, by Cell Signaling Technology (2 mentions) Af 401, by R&D Systems (2 mentions) Phospho p38 mapk t180 y182, by Cell Signaling Technology (2 mentions) Phospho mlkl, by Abcam (2 mentions) Ripk1, by Cell Signaling Technology (2 mentions)

Close spelling variants of this product

ERK1/2 (1487 citations) P42/44 MAPK (16 citations) ERK1/2 MAPK (3 citations) Phospho-p42/44 MAPK (Erk1/2) (3 citations)

2 protocols using p42 44 mapk erk1 2

1

Neutrophil Lysis and Protein Analysis

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Neutrophils were lysed in 1% (v/v) Triton X-100, 1% (w/v) sodium deoxycholate, 0.1% (w/v) SDS, 10 mM NaF, 1 mM sodium vanadate, 1 mM phenylmethanesulphonylfluoride in 10 mM Tris-HCl, pH 7.5, 150 mM NaCl and Complete Protease Inhibitor Cocktail (Roche). Cell lysates and supernatants were analyzed using antibodies specific to IL-1β/IL-1F2 (R&D; AF-401), IL-1α/IL-1F1 (R&D; AF-400), RIPK1 (Cell Signaling Technology; D94C12), SHP-1 (Cell Signaling Technology; C14H6), p38 MAPK (Cell Signaling Technology; D94C12), phospho-p38 MAPK (T180/Y182) (Cell Signaling Technology; 9211), Myosin-9 (Cell Signaling, #3403), p42/44 MAPK (ERK1/2) (Cell Signaling Technology; 137F5), or phospho-MLKL (S345) (Abcam; EPR9515[2]). Westerns were developed (ECL Substrate), visualized (ChemiDoc), and analyzed (ImageLab Software) with BioRad products.
Speir M., Nowell C.J., Chen A.A., O’Donnell J.A., Shamie I.S., Lakin P.R., D’Cruz A.A., Braun R.O., Babon J.J., Lewis R.S., Bliss-Moreau M., Shlomovitz I., Wang S., Cengia L.H., Stoica A.I., Hakem R., Kelliher M.A., O’Reilly L.A., Patsiouras H., Lawlor K.E., Weller E., Lewis N.E., Roberts A.W., Gerlic M, & Croker B.A. (2019). Ptpn6 inhibits caspase-8- and Ripk3/Mlkl-dependent inflammation. Nature immunology, 21(1), 54-64.
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2

Neutrophil Lysis and Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Neutrophils were lysed in 1% (v/v) Triton X-100, 1% (w/v) sodium deoxycholate, 0.1% (w/v) SDS, 10 mM NaF, 1 mM sodium vanadate, 1 mM phenylmethanesulphonylfluoride in 10 mM Tris-HCl, pH 7.5, 150 mM NaCl and Complete Protease Inhibitor Cocktail (Roche). Cell lysates and supernatants were analyzed using antibodies specific to IL-1β/IL-1F2 (R&D; AF-401), IL-1α/IL-1F1 (R&D; AF-400), RIPK1 (Cell Signaling Technology; D94C12), SHP-1 (Cell Signaling Technology; C14H6), p38 MAPK (Cell Signaling Technology; D94C12), phospho-p38 MAPK (T180/Y182) (Cell Signaling Technology; 9211), Myosin-9 (Cell Signaling, #3403), p42/44 MAPK (ERK1/2) (Cell Signaling Technology; 137F5), or phospho-MLKL (S345) (Abcam; EPR9515[2]). Westerns were developed (ECL Substrate), visualized (ChemiDoc), and analyzed (ImageLab Software) with BioRad products.
Speir M., Nowell C.J., Chen A.A., O’Donnell J.A., Shamie I.S., Lakin P.R., D’Cruz A.A., Braun R.O., Babon J.J., Lewis R.S., Bliss-Moreau M., Shlomovitz I., Wang S., Cengia L.H., Stoica A.I., Hakem R., Kelliher M.A., O’Reilly L.A., Patsiouras H., Lawlor K.E., Weller E., Lewis N.E., Roberts A.W., Gerlic M, & Croker B.A. (2019). Ptpn6 inhibits caspase-8- and Ripk3/Mlkl-dependent inflammation. Nature immunology, 21(1), 54-64.
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