Liver tissues were collected and routinely embedded in optimal cutting tissue. Frozen liver samples were sliced into 14-μm sections. For immunofluorescence staining, after being washed with phosphate-buffered saline (PBS) 3 times, the sections were penetrated with methanol at -20ºC for 10 minutes and sealed with 5% goat serum at room temperature for 1 hour. Finally, frozen liver sections were stained with CD68 (ab201340; Abcam, Cambridge, UK), CD11b (ab52478; Abcam), Ptgs2 (ab15191; Abcam), DMT1 (ab55812; Abcam), GPX4 (ab125066; Abcam), F4/80 (DF2789; Affinity, Jiangsu, China), TGFβ1 (ER31210; huabio, Hangzhou, China; 21898-1-AP; Proteintech), Ki67 (9129s; Cell Signaling Technology, Boston, MA), GSK3β (9832s; Cell Signaling Technology), P-GSK3β (5558p; Cell Signaling Technology), Nrf2 (16396-1; Proteintech, Chicago, IL), and iNOS (ab178945; Abcam) overnight at 4°C. After extensive washing, the frozen sections were incubated with the respective fluorescent secondary antibodies. Finally, the nucleus was stained with 4′,6-diamidino-2-phenylindole for 10 minutes.
F4 80 df2789
Manufactured by Affinity Biosciences
Sourced in China, United States
F4/80 (DF2789) is an antibody product offered by Affinity Biosciences. It is a laboratory tool used for the detection and analysis of the F4/80 antigen, which is a glycoprotein expressed on the surface of mature mouse macrophages.
Automatically generated - may contain errors
Lab products found in correlation
Ab201340, by Abcam (1 mentions)
Ab52478, by Abcam (1 mentions)
Ab55812, by Abcam (1 mentions)
Ab15191, by Abcam (1 mentions)
Ab178945, by Abcam (1 mentions)
Ab125066, by Abcam (1 mentions)
P gsk3β, by Cell Signaling Technology (1 mentions)
Er31210, by Huabio (1 mentions)
Gsk3β, by Cell Signaling Technology (1 mentions)
Dab solution, by Beijing Strong Biotechnologies (1 mentions)
Myog 67082 1 ig, by Proteintech (1 mentions)
Af7584, by Affinity Biosciences (1 mentions)
L9393, by Merck Group (1 mentions)
Confocal microscope, by Nikon (1 mentions)
2 protocols using f4 80 df2789
1
Immunofluorescence Analysis of Liver Tissue
2
Immunohistochemical Analysis of Muscle Tissue
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TA muscle sections were fixed with 4% PFA and permeabilized with 0.2% Triton X-100 for 10 min in PBS. Then, sections were incubated with 3% H2O2 for 20 min to remove the endogenous peroxidase and blocked with 10% normal goat serum for 2 h at room temperature. Sections were incubated with primary Ab against eMyHC (F1.652, Developmental Studies Hybridoma Bank, Iowa City, IA, USA; 1:50), laminin (L9393, Sigma-Aldrich; 1:100), Pax7 (AF7584, Affinity Biosciences; 1:300), F4/80 (DF2789, Affinity Biosciences; 1:500) or MyoG (67082-1-Ig, Proteintech; 1:300) at 4°C overnight. For immunofluorescence, sections were treated with FITC- or TRITC-conjugated secondary Abs for 2 h and DAPI for 10 min to observe the muscle with a confocal microscope (Nikon). For immunohistochemistry, sections were incubated with biotinylated goat secondary Ab specific to the host species at room temperature for 1 h, and then visualized by DAB solution (MXB Biotechnologies, Fuzhou, China) for 5 min. The images were captured using a phase-contrast microscope.
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