BP (C12H12O2, 95%) was purchased from Alfa Aesar (Ward Hill, NY, USA). Dimethyl sulfoxide (DMSO), crystal violet, [3-(4,5-dimethylthizol-2-yl)-2,5-diphenyltetrazolium bromide] (MTT), Tween-20, methanol, and horseradish peroxidase-conjugated secondary antibodies were purchased from Sigma Chemical Co. (St. Louis, MO, USA). The antibodies were all purchased from Cell Signaling Technology, Inc., (Danvers, MA, USA). Polyvinyldenefluoride (PVDF) membranes, BSA protein assay kit and chemiluminescence reagents were purchased from Amersham Biosciences (Arlington Heights, IL, USA).
3 4 5 dimethylthizol 2 yl 2 5 diphenyltetrazolium bromide mtt
Manufactured by Merck Group
Sourced in United States
[3-(4,5-dimethylthizol-2-yl)-2,5-diphenyltetrazolium bromide] (MTT) is a chemical compound used in cell biology research. It is a yellow tetrazolium salt that can be reduced to a purple formazan product by the mitochondrial enzymes of living cells.
Automatically generated - may contain errors
Lab products found in correlation
Chemiluminescence reagent, by Cytiva (1 mentions)
Polyvinyldenefluoride pvdf membranes, by Cytiva (1 mentions)
Bsa protein assay kit, by Cytiva (1 mentions)
Horseradish peroxidase conjugated secondary antibody, by Merck Group (1 mentions)
Tween 20, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
S camptothecin cpt, by Merck Group (1 mentions)
Ara c, by Merck Group (1 mentions)
Cisplatin, by Merck Group (1 mentions)
Etoposide, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Penicillin, by HiMedia (1 mentions)
Streptomycin, by HiMedia (1 mentions)
3 protocols using 3 4 5 dimethylthizol 2 yl 2 5 diphenyltetrazolium bromide mtt
1
Biological Assays with Purchased Reagents
2
Cytotoxicity Screening of Anticancer Drugs
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Etoposide (VP-16), teniposide (VM-26), 5-Fluorouracil (5-FU), doxorubicin hydrochloride (DXR), cytosine β-D-arabinofuranoside (Ara-C), methotrexate hydrate (MTX), cyclophosphamide monohydrate), Cisplatin and (S)-(+)-Camptothecin (CPT), 3-[4,5-Dimethyl-thizol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) were pursed from Sigma-Aldrich Co., St. Louis MO. Drugs were dissolved in dimethyl sulfoxide to 100 mM stock solutions that were stored at 4°C. These were diluted in water to 1 mM working stocks prior to the assays.
3
Cytotoxicity Evaluation of Compounds
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Dulbecco's modified eagle medium (DMEM), fetal calf serum, penicillin, streptomycin, and trypsin/ethylenediamine tetraacetic acid (EDTA) were purchased from HiMedia. Dimethyl sulfoxide and 3-(4, 5-dimethyl thizol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) were purchased from Sigma Chemical Co. (St. Louis, MO, USA).
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