High pure pcr template preparation kit for genomic dna

Manufactured by Roche

Sourced in Switzerland

The High Pure PCR Template Preparation Kit is a laboratory equipment product designed for the isolation and purification of genomic DNA. It provides a reliable and efficient method to extract DNA from various sample types for use in further downstream applications, such as PCR (Polymerase Chain Reaction).

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Lab products found in correlation

Nanodrop 2000, by Thermo Fisher Scientific (2 mentions) Rq1 dnase, by Promega (1 mentions) Rneasy mini kit, by Qiagen (1 mentions) Goat anti mouse igg h l, by Promega (1 mentions) Non radioactive cytotoxicity assay kit, by Promega (1 mentions) O phenylenediamine dihydrochloride, by Merck Group (1 mentions) Trypan blue stain solution, by Bio-Rad (1 mentions) Proteinase k, by Roche (1 mentions) Pc12 cells, by American Type Culture Collection (1 mentions)

2 protocols using high pure pcr template preparation kit for genomic dna

Isolation and Quantification of Nucleic Acids

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To isolate total RNA or genomic DNA, cells were trypsinized and washed twice with phosphate-buffered saline (PBS). Then, the cell pellet was collected and stored at −70 °C prior to isolation of DNA and/or RNA.
Total RNA was isolated from 1 million cells using an RNeasy Mini Kit (Qiagen, Venlo, Netherlands) followed by treatment with DNAse RQ1 (Promega) according to the manufacturer’s protocol. Isolation of genomic DNA was performed using a High Pure PCR Template Preparation Kit for genomic DNA (Roche, Basel, Switzerland) according to the manufacturer’s protocol. The quality of RNA and DNA was analyzed by electrophoresis in a 1% agarose gel containing ethidium bromide. The amount of RNA and DNA was determined with a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific) at the absorption wavelength of 260 nm.

Antonova D.V., Alekseenko I.V., Siniushina A.K., Kuzmich A.I, & Pleshkan V.V. (2020). Searching for Promoters to Drive Stable and Long-Term Transgene Expression in Fibroblasts for Syngeneic Mouse Tumor Models. International Journal of Molecular Sciences, 21(17), 6098.

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Apoptosis Mechanism in PC12 Cells

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PC12 cells were purchased from the American Type Culture Collection (USA and Canada). Dulbecco's modified Eagle medium (DMEM), ribonuclease A and o-phenylenediamine dihydro chloride (OPD) were obtained from Sigma-Aldrich (St. Louis, MO, USA). NP 2 EO was purchased from Tokyo Chemical Industry Corporation (Tokyo, Japan). Fetal bovine serum (FBS) was obtained from HyClone (Rockville, MD USA). Monoclonal antibody against cytochrome c, and polyclonal antibody against Bcl-2 were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Nonradioactive cytotoxicity assay kit and purified horseradish peroxidase conjugate of goat anti-mouse IgG (H+L) were obtained from Promega (Madison, WI, USA). Polyclonal antibody against Bax was provided by Calbiochem (San Diego, CA, USA). Anti-rabbit immunoglobulin peroxidase conjugate was obtained from Amersham Bioscience UK Limited (UK). Amersham TM ECL TM western blotting detection reagents were obtained from GE Healthcare UK Limited (UK). The High Pure PCR template Preparation Kit for genomic DNA and Proteinase K was obtained from Roche Diagnostics (Basel, Switzerland). Trypan blue stain solution (0.5%) was obtained from Bio-Rad (Hercules, CA, USA). The Cytochrome c Releasing Apoptosis Assay kit was from Medical and Biological Laboratories Co., Ltd (Japan). All other chemicals were analytical grade.

Liu C., Sun Y., Song Y., Saito T, & Kurasaki M. (2016). Nonylphenol diethoxylate inhibits apoptosis induced in PC12 cells. Environmental toxicology, 31(11).

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