hESC lines H1 and H9 were obtained from WiCell Research Institute. iPS-MSC25 (link) (derived from human mesenchymal stem cells) and iPS-Fib225 (link) (derived from human dermal fibroblasts) were a kind gift from George Q. Daley at Children’s Hospital Boston. Essential 8 medium (E8)5 (link), 0.5 mM EDTA, Accutase, ProLong® Antifade reagents, LIVE/DEAD® Cell Viability staining kit, Click-iT® EdU Alexa Fluor® 594 Imaging Kit and Alkaline Phosphatase Live Stain kit were obtained from Life Technologies. Small molecules Y-27632 (ROCK inhibitor, or RI), SB431542, and LDN193189 were from Selleckchem. Matrigel was obtained from BD Biosciences. PNIPAAm-PEG polymer (Mebiol Gel) was from Cosmo Bio, USA. SCID Beige mice were from Charles River Laboratory. Finally, the following antibodies and dilutions were used: Oct4 and Nanog (Santa Cruz Biotech, 1:100); FOXA2 or HNF3β (Santa Cruz Biotech, 1:200); Nestin (Millipore, 1:200); αSMA (Abcom, 1:200).
Hnf3β
Manufactured by Santa Cruz Biotechnology
HNF3β is a protein that acts as a transcription factor, regulating the expression of genes involved in embryonic development and maintenance of differentiated cell types. It plays a key role in the development of the liver, pancreas, and other endoderm-derived organs.
Automatically generated - may contain errors
Lab products found in correlation
Scid beige mice, by Charles River Laboratories (1 mentions)
Hesc lines h1 and h9, by WiCell (1 mentions)
Matrigel, by BD (1 mentions)
Accutase, by Thermo Fisher Scientific (1 mentions)
Nestin, by Merck Group (1 mentions)
Click it edu alexa fluor 594 imaging kit, by Thermo Fisher Scientific (1 mentions)
Alkaline phosphatase live stain kit, by Thermo Fisher Scientific (1 mentions)
Foxa2, by Santa Cruz Biotechnology (1 mentions)
Nanog, by Santa Cruz Biotechnology (1 mentions)
Prolong anti fade reagents, by Thermo Fisher Scientific (1 mentions)
Sb431542, by Selleck Chemicals (1 mentions)
Ldn193189, by Selleck Chemicals (1 mentions)
Y 27632, by Selleck Chemicals (1 mentions)
Olig2, by Merck Group (1 mentions)
2 protocols using hnf3β
1
Directed Differentiation of Pluripotent Stem Cells
2
EB Differentiation with Retinoic Acid and Shh
Check if the same lab product or an alternative is used in the 5 most similar protocols
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ES cells (G4 strain) were cultured in ES maintenance medium, in suspension for 2 days to induce embryoid bodies (EB) formation. EBs were then cultured in differentiation media supplemented with all-trans-Retinoic Acid (2 μM RA) or 2 μM RA plus P150 or P450 derived from the Shh-HEK293T CM and 2 μM RA plus P150 or P450 derived from HEK293T CM was also used as control. An aliquot of the Shh containing pools was used for estimation of Shh in each fraction by western blotting and densitometry. After 3 days in neuronal differentiation medium, the EBs were disaggregated using trypsin and the single cell suspension was plated on 0.1% gelatin coated coverslip dishes and cultured overnight in neuronal maintenance medium for attachment and spreading to facilitate immuno-staining. Primary antibodies used for immunostaining include HNF3β (Goat, Santa Cruz, 1:50), Olig2 (Rabbit, Millipore, 1:50). Secondary antibodies Donkey anti-Goat (A488, 1:250, Molecular Probes) and Donkey anti-Rabbit (A568, 1:250, Molecular Probes).
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