The largest database of trusted experimental protocols

Pe conjugated anti sca 1 or pdgfrα antibodies

Manufactured by R&D Systems

The PE-conjugated anti-Sca-1 or PDGFRα antibodies are laboratory reagents used for the detection and analysis of Sca-1 or PDGFRα proteins in samples. These antibodies are conjugated with the fluorescent dye phycoerythrin (PE), allowing for the visualization and quantification of the target proteins in flow cytometry or other immunoassays.

Automatically generated - may contain errors

2 protocols using pe conjugated anti sca 1 or pdgfrα antibodies

1

Adipocyte Differentiation Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Glucose, insulin, T3, collagenase and Dulbecco’s modified Eagle’s medium (DMEM) were from Sigma-Aldrich (St. Louis, MO). Antibody against estrogen receptor α (ERα) and PE-conjugated anti-Sca-1 or PDGFRα antibodies were from R&D System (Minneapolis, MN). The anti-UCP1 antibody, FITC-conjugated antibody against CD29 or CD34, and mouse estradiol ELISA kit were purchased from the Abcam (Cambridge, MA). Anti-GAPDH antibody was from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). NuPAGE gels, SuperScript III reverse transcriptase and oligo(dT)12–18 primers were from Invitrogen (Carlsbad, CA). The mouse diabetes multiplex assay kit was from Bio-Rad (Hercules, CA). HF diet (60 kCal% from fat, 20 kCal% from protein, 20 kCal% from carbohydrate, energy density: 5.24 kCal/g, catalog number D12492) was from Research Diets, Inc (New Brunswick, NJ). Regular chow (17 kCal% from fat, 25 kCal% from protein, 58 kCal% from carbohydrate, energy density: 3.1 kCal/g, catalog number 7912) was from Harlan Laboratories (Madison, WI).
+ Open protocol
+ Expand
2

Adipocyte Differentiation Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Glucose, insulin, T3, collagenase and Dulbecco’s modified Eagle’s medium (DMEM) were from Sigma-Aldrich (St. Louis, MO). Antibody against estrogen receptor α (ERα) and PE-conjugated anti-Sca-1 or PDGFRα antibodies were from R&D System (Minneapolis, MN). The anti-UCP1 antibody, FITC-conjugated antibody against CD29 or CD34, and mouse estradiol ELISA kit were purchased from the Abcam (Cambridge, MA). Anti-GAPDH antibody was from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). NuPAGE gels, SuperScript III reverse transcriptase and oligo(dT)12–18 primers were from Invitrogen (Carlsbad, CA). The mouse diabetes multiplex assay kit was from Bio-Rad (Hercules, CA). HF diet (60 kCal% from fat, 20 kCal% from protein, 20 kCal% from carbohydrate, energy density: 5.24 kCal/g, catalog number D12492) was from Research Diets, Inc (New Brunswick, NJ). Regular chow (17 kCal% from fat, 25 kCal% from protein, 58 kCal% from carbohydrate, energy density: 3.1 kCal/g, catalog number 7912) was from Harlan Laboratories (Madison, WI).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!