Cryosections were made of the liver and spleen by embedding the tissue in Tissue-Tek OCT medium (Bayer). Sections of 5–6 µm were cut, affixed to poly-L-lysine–coated glass slides and kept at −20 °C before use. Sections were stained for human Lyve1 (Dilution 1/100; DakoCytomation, Heverlee, Belgium) as described earlier3 (link) or human albumin (dilution 1/100 Bethyl Laboratories, Montgomery, TX). Sections were embedded in mounting medium containing DAPI for nuclear staining. Images were taken using Leica SP8 confocal microscope.
For histology, tissues were processed and embedded in paraplast as described previously. Tissues were stained for human Lyve1 (1:100; DakoCytomation, Heverlee, Belgium), CD45 (dilution 1/250, clone HI30; eBioscience, Vienna, Austria) processed and counterstained with hematoxylin and eosin, for the Lyve1 staining only, as described.34 (link) Pictures were taken using a Olympus BX51 microscope.
For histology, tissues were processed and embedded in paraplast as described previously. Tissues were stained for human Lyve1 (1:100; DakoCytomation, Heverlee, Belgium), CD45 (dilution 1/250, clone HI30; eBioscience, Vienna, Austria) processed and counterstained with hematoxylin and eosin, for the Lyve1 staining only, as described.34 (link) Pictures were taken using a Olympus BX51 microscope.