Female BALB/c-A nude mice at 4–5 weeks of age were purchased from Charles River (Beijing, China). For intracranial xenograft tumors, 5 × 105 U118R cells (shNC or shZDHHC4) in 5 µL PBS were injected into the right frontal lobe of nude mice. In the animal experiment of TMZ treatment, 3 days after tumor cell transplantation, intraperitoneal injection of DMSO or TMZ was performed. TMZ (25 mg kg−1 d−1) or DMSO was injected daily for 30 days. After 45 days, the entire brain tissue was collected, perfused with 4% formalin, and fixed overnight. Paraffin-embedded sections were stained with hematoxylin and eosin. To calculate the tumor volume, we measured the maximum area of each tumor and calculated the tumor volume as length × width2 × 0.52. The time of natural death of mice was recorded, and survival curves were plotted according to the Kaplan–Meier method.
Female balb c a nude mice
Manufactured by Charles River Laboratories
Sourced in China
Female BALB/c-A nude mice are an inbred strain of athymic, hairless mice commonly used in biomedical research. They are characterized by a lack of functional T cells due to a genetic mutation, making them useful for studying the effects of human tumor xenografts and other applications requiring an immunodeficient model.
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2 protocols using female balb c a nude mice
1
Intracranial Xenograft Tumor Model in Nude Mice
2
Evaluating Therapeutic Efficacy in Xenograft Model
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Female Balb/cA nude mice (5-6 weeks old) were purchased from Charles River Laboratories (Beijing, China). All animal experiments were conducted in accordance with the Institutional Animal Care and Use Committee Guidelines of the Shanghai Institute of Materia Medica Chinese Academy of Sciences. Human cancer cells (from 1.0 × 10 7 to 3.0 × 10 7 cells/mL) were prepared in NaCl solution, mixed with an equal volume of Matrigel (BD Biosciences), and then transplanted subcutaneously into the right flank of the mice. When tumor volumes reached approximately 100 mm 3 , the mice were randomly allocated into vehicle and treatment groups (n = 5/group). The treatment groups were administered 143D once daily, BI3406 twice daily, 143D combined with BI3406, or MRTX849 alone once daily. Tumor volumes were monitored in two dimensions and calculated as (length × width 2 )/2. Two hours after the last dose, tumor tissues were dissected and analyzed by Western blotting.
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