For EPR spectroscopy, chemically reconstituted ThCfd1–HisThNbp35 complex (40 μM) was anaerobically reduced with sodium dithionite (200 μM). Samples were shock-frozen after incubation for 3 min. X-band EPR derivative spectra were recorded on a Bruker ELEXSYS E500 spectrometer equipped with a Bruker dual mode cavity (ER4116DM) and a helium flow cryostat (Oxford Instruments ESR 900). The microwave bridge was a high-sensitivity Super-X bridge (Bruker ER-049X) with integrated microwave frequency counter. The magnetic field controller (ER032T) was calibrated with a Bruker NMR field probe (ER035M). EPR simulations were performed with the self-made routine esim_gfit (by E.B.).
Er 049x
Manufactured by Bruker
The ER-049X is a high-performance electron paramagnetic resonance (EPR) spectrometer designed for advanced spectroscopic analysis. It provides researchers with a reliable and efficient tool for investigating the electronic structure and dynamics of paramagnetic species in a wide range of materials and samples.
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2 protocols using er 049x
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EPR Spectroscopy of ThCfd1–HisThNbp35 Complex
2
Continuous-wave EPR Spectroscopy Methodology
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Continuous-wave (cw) X-band EPR measurements
were performed on a Bruker E500 ELEXSYS spectrometer equipped with
the Bruker dual-mode cavity (ER4116DM) or a standard cavity (ER4102ST)
and an Oxford Instruments helium flow cryostat (ESR 900). The microwave
bridge was a high-sensitivity Super-X bridge (Bruker ER-049X) with
integrated microwave frequency counter. The magnetic field controller
(ER032T) was calibrated with a Bruker NMR field probe (ER035M). EPR
simulations have been done with our own routines, esim_gfit and esim_sx.
For spin quantitation, the experimental derivative spectra were numerically
integrated by using the routine eview, and the results were corrected
for their g value dependence for field-swept spectra
by using Aasa and Vänngård approximation,32 (link) i.e. dividing the integrals by the factor,
were performed on a Bruker E500 ELEXSYS spectrometer equipped with
the Bruker dual-mode cavity (ER4116DM) or a standard cavity (ER4102ST)
and an Oxford Instruments helium flow cryostat (ESR 900). The microwave
bridge was a high-sensitivity Super-X bridge (Bruker ER-049X) with
integrated microwave frequency counter. The magnetic field controller
(ER032T) was calibrated with a Bruker NMR field probe (ER035M). EPR
simulations have been done with our own routines, esim_gfit and esim_sx.
For spin quantitation, the experimental derivative spectra were numerically
integrated by using the routine eview, and the results were corrected
for their g value dependence for field-swept spectra
by using Aasa and Vänngård approximation,32 (link) i.e. dividing the integrals by the factor,
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