Agro-infiltrated leaves were detached from the plants and examined for fluorescence from different YFP, GFP or RFP fusion proteins under a Nikon TI-E + C2 confocal laser-scanning microscope (Nikon Microsystems, Watford, United Kingdom). The excitation wavelength was set at 514 nm for YFP and GFP, and 555 nm for RFP, and the emission wavelength was set at 520–550 nm for YFP and GFP, and 583 nm for RFP. The images presented in the figures are either single image or multi-layered images to achieve the maximum signal intensity. The statistics of GFP signals were determined using the unpaired two-tailed Student t-test.
Ti e c2 confocal laser scanning microscope
Manufactured by Nikon
Sourced in United Kingdom
The TI-E + C2 confocal laser-scanning microscope is a high-performance imaging system designed for advanced microscopy applications. It features a confocal optical system that provides optical sectioning and improved image contrast. The microscope utilizes a laser light source and a scanning mechanism to acquire detailed, high-resolution images of samples.
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Lab products found in correlation
3 protocols using ti e c2 confocal laser scanning microscope
1
Visualizing Fluorescent Protein Localization
2
Imaging Aging Muscle Cells in C. elegans
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A Nikon TiE-C2 confocal laser scanning microscope, equipped with a 40xWI Plan Apo objective (NA 1.20, water immersion) was used for imaging. Images (1024 x 512 pixels, 6.67 pixels per µM, scanner zoom 2x) were collected by exciting with a 488-nm solid state argon (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this preprint this version posted June 16, 2021. ; https://doi.org/10.1101/2021.06.16.448702 doi: bioRxiv preprint laser and detecting through a 525/50 nm bandpass filter. Laser power (1.25%), gain (82) and offset (5) were maintained constant throughout all experiments. Images were collected for 4 independent aging cohorts at specific time-points during aging (at day 1, 4, 6, 8, 12, 14 and 18 of adulthood) . Standardized image nomenclature was used to facilitate downstream analysis. We recommend using the following format: C01W01M01.nd2, in which C, W and M refers to the Condition, Worm and Myofilament, respectively. The total number of worms and muscle cells per condition are summarized in Table 1.
The copyright holder for this preprint this version posted June 16, 2021. ; https://doi.org/10.1101/2021.06.16.448702 doi: bioRxiv preprint laser and detecting through a 525/50 nm bandpass filter. Laser power (1.25%), gain (82) and offset (5) were maintained constant throughout all experiments. Images were collected for 4 independent aging cohorts at specific time-points during aging (at day 1, 4, 6, 8, 12, 14 and 18 of adulthood) . Standardized image nomenclature was used to facilitate downstream analysis. We recommend using the following format: C01W01M01.nd2, in which C, W and M refers to the Condition, Worm and Myofilament, respectively. The total number of worms and muscle cells per condition are summarized in Table 1.
3
Confocal Imaging of Muscle Aging in C. elegans
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A Nikon TiE-C2 confocal laser-scanning microscope, equipped with a 40× WI Plan Apo objective (1.20 NA, water immersion) was used for imaging. Images (1024×512 pixels, 6.67 pixels/µM, scanner zoom 2×) were collected by exciting with a 488 nm solid-state argon laser and detecting through a 525/50 nm bandpass filter. Laser power (1.25%), gain (82) and offset (5) were maintained constant throughout all experiments. Images were collected for four independent ageing cohorts at specific time points during ageing (at day 1, 4, 6, 8, 12, 14 and 18 of adulthood). In all individuals, body wall muscle cells from the midsection of the body were imaged as these cells allow for imaging with minimal distortion. Standardized image nomenclature was used to facilitate downstream analysis. We recommend using the following format: C01W01M01.nd2, in which C, W and M refers to the condition, worm and myofilament, respectively. The total numbers of worms and muscle cells per condition are summarized in Table 1 .
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