BV-2 cells were assayed as described above and supernatants collected after 48 h to assess in ammatory-related molecules using the CBA Mouse In ammation kit (BD Pharmingen, USA) according to the supplier´s protocol. For NO dosage, supernatants were processed as previously described [7] (link). In brief, supernatants were mixed (1:1) with 0.1% naphthyl ethylenediamine and 1% sulfanilamide in 5% phosphoric acid solution (Sigma-Aldrich, USA) for 5 min at room temperature in the dark. Absorbance was then measured at 540 nm using a spectrophotometer (SpectraMax Paradigm Multi-mode Microplate Reader, Molecular Devices, LLC, USA).
Cba mouse in ammation kit
Manufactured by BD
Sourced in United States
The BD CBA Mouse Inflammation Kit is a laboratory tool designed to measure the levels of specific cytokines and chemokines in mouse biological samples. It provides a quantitative analysis of these inflammatory markers, which are important in understanding the immune response and inflammatory processes in mouse models.
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2 protocols using cba mouse in ammation kit
1
Quantifying Inflammatory Mediators in BV-2 Cells
2
Quantifying Inflammatory Mediators in BV-2 Cells
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BV-2 cells were assayed as described above and supernatants collected after 48 h to assess in ammatory-related molecules using the CBA Mouse In ammation kit (BD Pharmingen, USA) according to the supplier´s protocol. For NO dosage, supernatants were processed as previously described [7] (link). In brief, supernatants were mixed (1:1) with 0.1% naphthyl ethylenediamine and 1% sulfanilamide in 5% phosphoric acid solution (Sigma-Aldrich, USA) for 5 min at room temperature in the dark. Absorbance was then measured at 540 nm using a spectrophotometer (SpectraMax Paradigm Multi-mode Microplate Reader, Molecular Devices, LLC, USA).
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