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Em 15300sxv system

Manufactured by JEOL
Sourced in United States

The EM-15300SXV system is a scanning electron microscope (SEM) manufactured by JEOL. It is designed for high-resolution imaging and analysis of a wide range of materials and samples. The system features advanced electron optics and detectors to provide detailed information about the surface morphology and composition of the specimen.

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2 protocols using em 15300sxv system

1

Preparation of Amyloid-beta Oligomers

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The iso-Aβ1–42 peptide was synthesized in the solid phase using tert-butyloxycarbonyl (Boc)-chemistry in-house, as reported earlier [113 (link)]. A stock solution of this peptide was prepared using distilled water, to yield a concentration of 1 mg/mL (200 μM, pH = 7), and it was sonicated for 3 min. The solution was incubated for 10 min at room temperature (RT), then the pH level was adjusted (pH = 11), and it was further incubated for 2 h. After a 3-min-long sonication process, the Aβ1–42 solution was diluted in phosphate buffer (PBS, 20 mM) to a final peptide concentration of 50 μM (26.67 mM phosphate, 1.2% NaCl, pH = 7.4). The solution was stored at 4 °C until further use on the same day.
The oligomeric state of the Aβ peptide was verified by a transmission electron microscope (JEM-1400, JEOL USA Inc., Peabody, MA, USA) operating at 120 kV. Images were taken by an EM-15300SXV system, routinely at a magnification of 25,000 and 50,000, and were processed by the SightX Viewer Software (EM-15300SXV Image Edit Software, JEOL Ltd., Tokyo, Japan).
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2

Visualizing Nanoparticle Aggregation

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A 250 μM solution of 6 and 8 in 20 mM phosphate buffer ( pH 6.5), containing 150 mM NaCl was placed on Formvar-carboncoated 400-mesh copper grids (Electron Microscopy Sciences, Washington, PA) and stained negatively with uranyl acetate. The aggregates were characterized by TEM on a JEOL JEM-1400 transmission electron microscope (JEOL USA Inc., MA, USA) operating at 120 kV. The images were taken with an EM-15300SXV system and processed with the SightX Viewer software (also JEOL) routinely at a magnification of ×25 000.
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