Western blotting was performed using standard methods. Protein concentration was measured using a BCA protein assay kit (Beyotime, China). Equal amounts of protein were separated by electrophoresis on 15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred electrophoretically onto PVDF membranes (0.22 um, Whatman Westran PVDF membrane, GE Healthcare Life Science, Germany). The membranes were blocked for 30 min with 5% nonfat milk. After blocking, the membranes were incubated overnight with anti-GAR1 (1:500, Sangon, China), anti-NHP2 (1:500, Sangon, China), anti-NOP10 (1:500, Cusabio, China), anti-DKC1 (1:1000, Signalway, China), and β-actin (1:2000, ABclonal, China) at 4 °C. After washing with TBST, the membrane was incubated for 1 h at room temperature with horseradish peroxidase-conjugated anti-rabbit IgG or anti-mouse IgG (1:2000, Proteintech, USA) in TBST. Finally, signals were developed using an enhanced chemiluminescence system kit.
Anti nop10
Manufactured by Cusabio
Sourced in China
Anti-NOP10 is a laboratory product designed for use in research applications. It functions as an antibody that specifically binds to the NOP10 protein. The core purpose of this product is to facilitate the detection and study of the NOP10 protein in experimental settings.
Automatically generated - may contain errors
Lab products found in correlation
Bca protein assay kit, by Beyotime (1 mentions)
β actin, by ABclonal (1 mentions)
Anti β actin, by Merck Group (1 mentions)
Anti dyskerin, by Santa Cruz Biotechnology (1 mentions)
Anti gar1, by Proteintech (1 mentions)
Tgx stain free fastcast acrylamide solutions, by Bio-Rad (1 mentions)
Image lab v5, by Bio-Rad (1 mentions)
Anti eif4gi, by Cell Signaling Technology (1 mentions)
Mini protean tgx stain free, by Bio-Rad (1 mentions)
Anti vimentin, by Cell Signaling Technology (1 mentions)
Lamin b, by Santa Cruz Biotechnology (1 mentions)
Anti gapdh, by Merck Group (1 mentions)
Chemidoc xrs imaging system, by Bio-Rad (1 mentions)
2 protocols using anti nop10
1
Western Blot Analysis of Ribonucleoprotein Complex
2
Western Blot Analysis of Nucleolar Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Protein solutions were diluted in Laemmli loading dye (2% SDS, 8% glycerol, 62.5 mM Tris-HCl pH 6.8, 0.005% bromophenol blue, and 2% β-mercaptoethanol) and separated on a 10% gel by SDS-PAGE (TGX Stain-Free™ FastCast™ Acrylamide Solutions; BIORAD) or 4-15% gel (Mini-PROTEAN® TGX Stain-Free™; BIORAD). Proteins were transferred to nitrocellulose membranes (Amersham Protran 0.2um nc) and probed overnight at 4°C with appropriate antibodies. Detection was performed with appropriate HRP-conjugated secondary antibodies and chemiluminescent detection was performed on a ChemiDoc XRS+ Imaging System (BIORAD) using ImageLab v5.1.1 (BIORAD). The Stain-Free system makes it possible for us to check the quality and quantity of the loaded proteins, especially for RIP and polysome protein gels. The following antibodies were used: anti-Dyskerin (Santa Cruz Biotechnology, sc-373956), anti-NHP2 (Santa Cruz Biotechnology, sc-398430), anti-NOP10 (Cusabio, CSB-PA873610LD01HU), anti-GAR1 (Proteintech, 11711-1-AP), anti-RPS14 (Santa Cruz Biotechnology, sc-68873), anti-RPL5 (Bethyl, A303-933A), anti-EIF4GI (Cell Signaling, 2858S), anti-Vimentin (Cell Signaling, 5741S), anti-GAPDH (Sigma-Aldrich, G8795), anti-b-Actin (Sigma-Aldrich, A2228), Lamin B (Santa Cruz Biotechnology, sc-6216).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!