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2 protocols using anti phospho bcl 2 thr56

1

Monoclonal Antibody-Based Protein Analysis

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Monoclonal anti-B-cell lymphoma-2 (Bcl-2, #sc-7382), anti-phospho-Bcl-2-Ser70 (# sc-293128), anti-BCL-XL (#sc8392), anti-p27 (#sc-393380), anti-MRP (#sc-365635), and anti-MCL-1 (#sc-69840) antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-actin antibody (#sc-8432), used as a control for western blotting (WB), was purchased from Santa Cruz Biotechnology (Heidelberg, Germany). Anti-phospho-Bcl-2-Thr56 (#2875) was purchased from Cell Signaling Technology (Denver, MA, USA). Anti-phospho-p27-Ser10 antibody (#346300) was purchased from Invitrogen. Rabbit polyclonal anti-SEMA3F (#AB5471P) was obtained from Chemicon International (Temecula, USA), and rabbit anti-cleaved Caspase3 (#9664) and anti-Caspase3 (#9662) were purchased from Cell Signaling Technology (Boston, MA, USA; 1:100). Anti-kinase-interacting stathmin (KIS) antibody (#AJ1743c) was obtained from Abgent (San Diego, CA, USA). HRP-conjugated secondary antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA).
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2

Comprehensive Antibody Toolkit for Apoptosis Analysis

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Monoclonal anti-B-cell lymphoma-2 (Bcl-2, #sc-7382), anti-phospho-Bcl-2-Ser70 (# sc-293128), anti-BCL-XL (#sc8392), anti-p27 (#sc-393380), anti-MRP (#sc-365635), and anti-MCL-1 (#sc-69840) antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-actin antibody (#sc-8432), used as a control for western blotting (WB), was purchased from Santa Cruz Biotechnology (Heidelberg, Germany). Anti-phospho-Bcl-2-Thr56 (#2875) was purchased from Cell Signaling Technology (Denver, MA, USA). Anti-phospho-p27-Ser10 antibody (#346300) was purchased from Invitrogen. Rabbit polyclonal anti-SEMA3F (#AB5471P) was obtained from Chemicon International (Temecula, USA), and rabbit anti-cleaved Caspase3 (#9664) and anti-Caspase3 (#9662) were purchased from Cell Signaling Technology (Boston, MA, USA; 1:100). Anti-kinase-interacting stathmin (KIS) antibody (#AJ1743c) was obtained from Abgent (San Diego, CA, USA). HRP-conjugated secondary antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA).
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