Enhanced chemiluminescence ecl solution kit

Materials and chemicals P. umbellatus was purchased from Kangmei Pharmaceutical Co., Ltd. (Guangdong, China). The RPMI 1640 medium, fetal bovine serum (FBS), and phosphate-buffered saline (PBS) were procured from Hyclone. Lipopolysaccharide (LPS) was procured from Sigma (MO, USA). Primers for real-time reverse transcriptasepolymerase chain reaction (RT-PCR) and TRIzol were purchased from Invitrogen. Revert Aid First-Strand cDNA Synthesis Kit and FastStart Universal SYBR Green Master (ROX) were purchased from Roche. RIPA and primary antibodies for Western blot analysis, including anti-IκB, anti-phospho-IκB, anti-NF-κB p65, anti-JAK2, anti-phospho-JAK2, and anti-GAPDH, were purchased from Cell Signaling Technology. Secondary antibodies were obtained from Sigma. The Enhanced Chemiluminescence (ECL) Solution Kit was obtained from Millipore (MA, USA). APC mouse anti-human CD14, APC mouse anti-human CD16, APC mouse anti-human CD40, FITC mouse anti-human CD86, FITC mouse anti-human CD68, and FITC mouse anti-human CD23 were purchased from BD Systems (BD Biosciences, USA).

Materials and chemicals P. umbellatus was purchased from Kangmei Pharmaceutical Co., Ltd. (Guangdong, China). The RPMI 1640 medium, fetal bovine serum (FBS), and phosphate-buffered saline (PBS) were procured from Hyclone. Lipopolysaccharide (LPS) was procured from Sigma (MO, USA). Primers for real-time reverse transcriptase-polymerase chain reaction (RT-PCR) and TRIzol were purchased from Invitrogen. Revert Aid First-Strand cDNA Synthesis Kit and FastStart Universal SYBR Green Master (ROX) were purchased from Roche. RIPA and primary antibodies for Western blot analysis, including anti-IκB, anti-phospho-IκB, anti-NF-κB p65, anti-JAK2, anti-phospho-JAK2, and anti-GAPDH, were purchased from Cell Signaling Technology. Secondary antibodies were obtained from Sigma. The Enhanced Chemiluminescence (ECL) Solution Kit was obtained from Millipore (MA, USA). APC mouse anti-human CD14, APC mouse antihuman CD16, APC mouse anti-human CD40, FITC mouse anti-human CD86, FITC mouse anti-human CD68, and FITC mouse anti-human CD23 were purchased from BD Systems (BD Biosciences, USA).

The RPMI 1640 medium, fetal bovine serum (FBS), and phosphate-buffered saline (PBS) were procured from Hyclone. Lipopolysaccharide (LPS) was procured from Sigma (MO, USA). Primers for real-time reverse transcriptase-polymerase chain reaction (RT-PCR) and TRIzol were purchased from Invitrogen.
Revert Aid First-Strand cDNA Synthesis Kit and FastStart Universal SYBR Green Master (ROX) were purchased from Roche. RIPA and primary antibodies for Western blot analysis, including anti-IκB, antiphospho-IκB, anti-NF-κB p65, anti-JAK2, anti-phospho-JAK2, and anti-GAPDH, were purchased from Cell Signaling Technology. Secondary antibodies were obtained from Sigma. The Enhanced Chemiluminescence (ECL) Solution Kit was obtained from Millipore (MA, USA). APC mouse anti-human CD14, APC mouse anti-human CD16, APC mouse anti-human CD40, FITC mouse anti-human CD86, FITC mouse anti-human CD68, and FITC mouse anti-human CD23 were purchased from BD Systems (BD Biosciences, USA).