Esquire model 6000

The HPLC-ESI-MS n analysis was performed on a Dionex ultimate 3000 series instrument (Thermo Scientific Inc.) equipped with a binary pump, an autosampler and a column compartment (kept at 30 C) and coupled with a Bruker Esquire model 6000 ion trap mass spectrometer with an ESI source (Bremen, Germany). Analysis was carried out on a Phenomenex Gemini C 18 column (5 mm, 250 Â 3.0 mm i.d.) using the same conditions as previously (Gouveia and Castilho, 2012c) . Extract solutions (5 mg mL À1 ) were prepared by dissolving the dry extract (DE) in the initial HPLC mobile phase. After filtration through 0.45 mm PTFE membrane filters, 5 mL was injected.

The chromatographic separation was achieved using a Dionex ultimate 3000 series instrument (Thermo Scientific Inc.) equipped with a binary pump, an autosampler, a column compartment (kept at 30 ºC) and a diode array detector (DAD) hyphenated to a Bruker Esquire model 6000 ion trap mass spectrometer (Bremen, Germany). Separation was carried out on a Phenomenex Gemini C 18 column (5 µm, 250 x 3.0 mm i.d.) using the same conditions as previously (Spínola et al., 2014) .

The HPLC-ESI-MS n analysis was performed on a Dionex ultimate 3000 series instrument (Thermo Scientific Inc.) equipped with a binary pump, an autosampler, a column compartment (kept at 30 ºC) and coupled with a Bruker Esquire model 6000 ion trap mass spectrometer (Bremen, Germany). Separation was carried out on a Phenomenex Gemini C 18 column (5 µm, 250 x 3.0 mm i.d.) using the same conditions reported in (Spínola et al., 2014) . Dry extracts (DE) from fruits and leaves were redissolved in the initial HPLC mobile phase (5 mg mL -1 ), filtered through 0.45 µm PTFE