Sequant zic philic 5 μm polymeric 150 2.1 mm peek coated hplc column

Manufactured by Merck Group

Sourced in United States

The SeQuant® ZIC®-pHILIC 5 μm polymeric 150 × 2.1 mm PEEK-coated HPLC column is a high-performance liquid chromatography (HPLC) column designed for the separation and analysis of polar and hydrophilic compounds. The column features a zwitterionic (ZIC) stationary phase and a particle size of 5 μm. The column is coated with PEEK (polyether ether ketone) for chemical and physical durability.

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SeQuant ZIC-pHILIC 2.1 × 150 mm (5 μm SeQuant ZIC-pHILIC polymeric column ZIC-pHILIC 150 × 2.1 mm ( SeQuant ZIC-pHILIC column SeQuant Zic-pHilic ZIC-pHILIC column ZIC-pHILIC SeQuant MM.1S

2 protocols using sequant zic philic 5 μm polymeric 150 2.1 mm peek coated hplc column

HILIC-based HPLC-MS Metabolite Profiling

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The mass spectrometer was coupled to a Shimadzu HPLC (Nexera X2 LC-30AD). Chromatography was performed under HILIC conditions using a SeQuant^® ZIC^®-pHILIC 5 μm polymeric 150 × 2.1 mm PEEK-coated HPLC column from MilliporeSigma, USA. The column temperature, sample injection volume, and flow rate were set to 45 °C, 5 μL, and 0.15 mL/min, respectively. The HPLC conditions were as follows: Solvent A: 20 mM ammonium carbonate including 0.1% ammonium hydroxide, and Solvent B: acetonitrile. The gradient conditions were as follows: 0 min: 80% B, 20 min: 20% B, 21 min: 80% B, and 34 min: 80% B. The total run time was 34 min.

Doke M., McLaughlin J.P., Baniasadi H, & Samikkannu T. (2022). Sleep Disorder and Cocaine Abuse Impact Purine and Pyrimidine Nucleotide Metabolic Signatures. Metabolites, 12(9), 869.

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Quantitative Metabolite Profiling by HILIC-HPLC

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Chromatography was performed under HILIC conditions using a SeQuant® ZIC®-pHILIC 5 μm polymeric 150 × 2.1 mm PEEK coated HPLC column, MilliporeSigma, USA. The column temperature, sample injection volume, the flow rate was set to 45 °C, 5 μL, and 0.15 mL/min respectively. The HPLC conditions were as follows: Solvent A: 20 mM ammonium carbonate including 0.1% Ammonium hydroxide. Solvent B: Acetonitrile. Gradient condition was 0 min: 80% B, 20 min: 20% B, 20.5 min 80% B, 34 min: 80% B. Total run time: 34 mins. Data was processed by SCIEX MultiQuant 3.0.3 software with relative quantification based on the peak area of each metabolite.

Zeinert R.D., Baniasadi H., Tu B.P, & Chien P. (2020). The Lon protease links nucleotide metabolism with proteotoxic stress. Molecular cell, 79(5), 758-767.e6.

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