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2 protocols using 2 kd mwco slide a lyzer dialysis cassettes

1

Screening Organic Acids and Peroxidase Activity

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The chemicals, their sources, purity, and CAS #s were: adipic acid, TCI, ≥ 99%, 124-04-9; ammonium nitrate, Fisher, ACS grade, 6484-52-2; citric acid monohydrate, Fisher, ACS grade, 5949-29-1; DL-3-hydroxybutyric acid sodium salt, Chem Impex Int’l Inc., 100.30%, 150-83-4 & 306-31-0; DL-malic acid, Alfa Aesar, 98%, 6915-15-7; glutaric acid, Acros organics, 99%, 110-94-1; hydrogen peroxide 3% W/W, BDH chemicals, 7722-84-1; horseradish peroxide type II, Sigma, 150-250 U/mg, 9003-99-0; lactic acid, TCI, ≥ 85%, 50-21-5; pimelic acid, Alfa Aesar, 98+%, 111-16-0; sodium acetate, VWR, ACS grade, 127-09-3; sodium azide, Sigma, 99.8%, 26628-22-8; sodium nitrate, BDH chemicals, ACS grade, 7631-99-4; succinic acid, TCI America, ≥ 99%,110-15-6; and tricarballylic acid, Alfa Aesar, 98%, 99-14-9. For electron microscopy, hexagonal copper square grids with 200 mesh carbon support film from Electron Microscopy Sciences were used. Pierce Biotechnology’s 2 kD MWCO Slide-A-Lyzer™ dialysis cassettes were used.
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2

Analytical Characterization of Organic Compounds

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The chemicals, their sources, purity, and CAS numbers were adipic acid,
TCI, ≥ 99%, 124-04-9; ammonium nitrate, Fisher, ACS grade, 6484-52-2;
citric acid monohydrate, Fisher, ACS grade, 5949-29-1; DL-3-hydroxybutyric acid
sodium salt, Chem Impex Int’l Inc., 100.30%, 150-83-4 & 306-31-0;
DL-malic acid, Alfa Aesar, 98%, 6915-15-7; glutaric acid, Acros organics,
99%,110-94-1; hydrogen peroxide 3% W/W, BDH chemicals, 7722-84-1; horseradish
peroxide type II, Sigma, 150-250 U/mg, 9003-99-0; lactic acid, TCI, ≥
85%, 50-21-5; pimelic acid, Alfa Aesar, 98+%, 111-16-0; sodium acetate, VWR, ACS
grade, 127-09-3; sodium azide, Sigma, 99.8%, 26628-22-8; sodium nitrate, BDH
chemicals, ACS grade, 7631-99-4; succinic acid, TCI America, ≥
99%,110-15-6; and tricarballylic acid, Alfa Aesar, 98%, 99-14-9. The electron
microscopy grids were 200 mesh carbon support film on hexagonal copper square
grids from Electron Microscopy Sciences. Pierce Biotechnology’s 2 kD MWCO
Slide-A-Lyzer™ dialysis cassettes were used. Trace metal grade
concentrated nitric acid was from Fisher. ICP/DCP Ce ion standard solution was
from Aldrich.
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