The plasma samples were prepared by centrifuging the collected blood samples at 1000 × g at 4°C for 10 min (Sun et al., 2016 ). The concentrations of immunoglobulin (Ig ) A, IgG, and IgM and endotoxin in plasma were measured with ELISA kits (A40199-S, A04022-S, A02721-S, A049165-S; Shanghai Jining Shiye Co. Ltd., Shanghai, China), according to the manufacturer's instructions. The activities of α-amylase, lipase, and chymotrypsin in chyme from the duodenum and jejunum were determined by a colorimetric method using specific assay kits (C016-1, A054-1, A080-1; Nanjing Jiancheng Bioengineering Institute of China, Nanjing, China).
C016 1
Manufactured by Nanjing Jiancheng
Sourced in China
The C016-1 is a laboratory equipment used for measuring and analyzing various properties of samples. It is a versatile and reliable instrument designed to provide accurate and consistent results.
Automatically generated - may contain errors
2 protocols using c016 1
1
Characterization of Plasma Immunoglobulins and Digestive Enzymes
2
Antioxidant and Intestinal Function Analysis
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Total antioxidant capacity (T-AOC), glutathione peroxidase (GPX), catalase (CAT) and superoxide dismutase (SOD) activities and malondialdehyde (MDA) and protein carbonyl (PC) concentrations were determined by their specific assay kits (A015-1-2, A005-1-2, A007-1-1, A001-3-2, A003-1-2, and A087-1-2) from the Nanjing Jiancheng Bioengineering Institute of China. Protein concentration was analyzed by the bicinchoninic acid assay [19 (link)]. Jejunal chyme lipase, α-amylase, and neutral protease activities were measured by their specific assay kits (A054-1, C016-1, A080-3-1; Nanjing Jiancheng Bioengineering Institute, Nanjing, China) [20 (link)]. Small intestinal histopathology and morphology were analyzed as previously described [18 (link)]. Briefly, the duodenum, jejunum, and ileum tissues were washed with saline, fixed in 10% neutral buffered formalin, embedded in paraffin, sectioned at 5 μm, and then stained with hematoxylin and eosin. All slides were examined by a microscope.
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