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3 protocols using manganese chloride

1

Bacterial Enzyme Production Protocol

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Bovine serum albumin, tyrosine, thrombin, and standard streptokinase were obtained from Sigma Aldrich (Bengaluru, India). Starch casein agar, ISP broth, glucose, yeast extract, ammonium sulfate, manganese chloride, sodium carbonate, sodium bicarbonate, magnesium sulfate, sodium acetate, sodium chloride, sodium hydroxide, and ferrous sulfate were purchased from Hi Media laboratories (Mumbai, India). Copper sulfate, Folin–Ciocalteau reagent, potassium dihydrogen phosphate, trichloroacetic acid (TCA), hydrochloric acid (HCl), ethylene-diaminetetraacetic acid (EDTA), and Tris-HCl were purchased from Sisco research laboratories (SRL, Mumbai, India).
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2

Analytical-Grade Chemical Synthesis

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Silver nitrate (AgNO3) as analytical grade, citric acid, ferric ammonium citrate, sodium nitrite, magnesium sulphate, calcium chloride, sodium carbonate, EDTA disodium salt, zinc sulphate, manganese chloride, copper sulphate, sodium molybdate, and cobalt nitrate were purchased from Himedia Laboratories Pvt., Ltd., while dipotassium hydrogen phosphate and boric acid were purchased from SRL (Mumbai, India).
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3

Synthesis of Metal-Protein Conjugates

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Auric chloride (AuCl3), EST from
porcine liver, and rhodamine 6G were procured from Sigma-Aldrich (USA).
Various metal ion salts were purchased from different vendors, that
is, mercuric chloride, nickel(II) sulfate, zinc(II) acetate, and tin
chloride from Sigma-Aldrich, USA; calcium chloride, cobalt chloride,
sodium selenite, copper sulfate, aluminium sulfate, ferric chloride,
and manganese chloride from Himedia, India; cadmium chloride from
Qualigenes, India; magnesium chloride from, CDH, New Delhi, India;
and lead(II) acetate from Merck, India. Deionized water (Viola), NaOH
(Avra, India) and HCl (RANKEM, India) were used in the experiments.
Various other analytical grade chemicals and organic solvents were
purchased from the suitable vendors.
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