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Edta coated microtainers

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EDTA-coated microtainers are small, sterile containers used for the collection and storage of blood samples. The interior of the microtainers is coated with EDTA, an anticoagulant that helps prevent the blood from clotting during the collection and transportation process.

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Lab products found in correlation

Qiaamp circulating nucleic acid kit, by Qiagen (2 mentions) Serum separator sst microtainers, by BD (1 mentions) Ast elisa kit, by Cloud-Clone (1 mentions) Vetscan vs2, by Abaxis (1 mentions) Advia120 hematology analyzer, by Bayer (1 mentions) Adiponectin, by Abcam (1 mentions) Cholesterol, by Fujifilm (1 mentions) Insulin, by Mercodia (1 mentions)

Spelling variants of this product

BD Microtainer (240 citations) EDTA-coated microtainer tubes (19 citations) EDTA microtainers (7 citations)

5 protocols using edta coated microtainers

1

Measuring Circulating Mitochondrial DNA

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Mouse blood was collected by cardiac puncture into EDTA-coated microtainers (BD). Plasma was obtained after two rounds of centrifugation (3000 × g, 10 min at 4°C). Two hundred and fifty microliters of plasma from each mouse was used to extract circulating DNA using QIAamp circulating nucleic acid kit (Qiagen). One twentieth of the total yield from each plasma sample was used for real-time PCR to assess levels of mitochondrial DNA using six sets of mouse mitochondrial DNA primers as described above.
Liu Y., Nguyen P.T., Wang X., Zhao Y., Meacham C.E., Zou Z., Bordieanu B., Johanns M., Vertommen D., Wijshake T., May H., Xiao G., Shoji-Kawata S., Rider M.H., Morrison S.J., Mishra P, & Levine B. (2020). Tlr9 and Beclin 1 Cross-Talk Regulates Muscle AMPK Activation in Exercise. Nature, 578(7796), 605-609.
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2

Comprehensive Murine Blood Analysis Protocol

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Peripheral blood was collected by cardiac puncture into EDTA-coated microtainers (BD, Biosciences Canada, Mississauga, Canada). Blood samples were inserted into a Hemavet (Drew Scientific Group, Waterbury CT) for complete blood count (CBC) analyses. For serum separation, mouse blood was collected via cardiac puncture into serum separator SST microtainers (BD Biosciences). Sample tubes were gently inverted and the blood was allowed to clot at room temperature for 30 minutes. Samples were then centrifuged at 1200 g for 10 minutes. The serum portion was immediately collected and stored in −80 °C until use. Mouse cytokine levels were measured on collected serum with the Cytokine-20-Plex mouse panel (Thermo Scientific Pierce, Waltham, MA) following the manufacturer’s instructions. Luminescence was measured on the Luminex 100 instrument (Luminex, Austin, TX). Data with a low bead count (<45 beads) was omitted. Additionally, we measured MCP-3 and MCP-5 in mouse serum with MCP-3 and MCP-5 ELISA kits (Thermo Scientific, Waltham, MA). Serum metabolites and liver enzymes in serum were measured using the VetScan Comprehensive Diagnostic Profile and Mammalian Liver Profile (Abaxis Union City, CA) on the Abaxis VetScan VS2 (Abaxis Union City, CA). An AST ELISA kit (Cloud-Clone Corp. Wuhan China) was used to assay for serum amino aspartate transferase (AST) levels.
Yu F.P., Dworski S, & Medin J.A. (2018). Deletion of MCP-1 Impedes Pathogenesis of Acid Ceramidase Deficiency. Scientific Reports, 8, 1808.
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3

Measuring Circulating Mitochondrial DNA

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Mouse blood was collected by cardiac puncture into EDTA-coated microtainers (BD). Plasma was obtained after two rounds of centrifugation (3000 × g, 10 min at 4°C). Two hundred and fifty microliters of plasma from each mouse was used to extract circulating DNA using QIAamp circulating nucleic acid kit (Qiagen). One twentieth of the total yield from each plasma sample was used for real-time PCR to assess levels of mitochondrial DNA using six sets of mouse mitochondrial DNA primers as described above.
Liu Y., Nguyen P.T., Wang X., Zhao Y., Meacham C.E., Zou Z., Bordieanu B., Johanns M., Vertommen D., Wijshake T., May H., Xiao G., Shoji-Kawata S., Rider M.H., Morrison S.J., Mishra P, & Levine B. (2020). Tlr9 and Beclin 1 Cross-Talk Regulates Muscle AMPK Activation in Exercise. Nature, 578(7796), 605-609.
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4

Blood Collection and Analysis in Mouse Models

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Blood was collected from the tail vein of PBS, trastuzumab and T-Sia 2-treated mice (Fig. 5) into EDTA-coated Microtainers (BD Biosciences) 48 h after the first dose of treatment. Generally, 40–60 μl of blood were diluted to a total volume of 240 μl using 0.9% NaCl. Complete blood counts were measured on the ADVIA120 Hematology Analyzer using the Multispecies v.5.9.0-MS software (Bayer) and adjusted to the respective dilution factor.
Gray M.A., Stanczak M.A., Mantuano N.R., Xiao H., Pijnenborg J.F., Malaker S.A., Miller C.L., Weidenbacher P.A., Tanzo J.T., Ahn G., Woods E.C., Läubli H, & Bertozzi C.R. (2020). Targeted glycan degradation potentiates the anticancer immune response in vivo. Nature chemical biology, 16(12), 1376-1384.
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5

Metabolic Profiling of Fasted Mice

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Several groups of mice were killed by decapitation 4 hours after food withdrawal at 7:00 am, and trunk blood was collected from the site where the animal was decapitated into EDTA-coated microtainers (BD, Franklin Lakes, NJ) and kept in ice until centrifugation to separate plasma. Plasma was used to determine levels of nonesterified fatty acid, TG, cholesterol (Wako Diagnostics, Richmond, VA), insulin (Mercodia, Uppsala, Sweden), adiponectin (Abcam, Cambridge, MA), and ALT (Pointe Scientific, Canton, MI). To assess hepatic TG content, hepatic lipids were extracted from frozen livers in isopropanol, and TG was measured as previously published.37 (link)
Lee S.M., Pusec C.M., Norris G.H., De Jesus A., Diaz-Ruiz A., Muratalla J., Sarmento-Cabral A., Guzman G., Layden B.T, & Cordoba-Chacon J. (2021). Hepatocyte-Specific Loss of PPARγ Protects Mice From NASH and Increases the Therapeutic Effects of Rosiglitazone in the Liver. Cellular and Molecular Gastroenterology and Hepatology, 11(5), 1291-1311.
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