Mmp 1 antibody

6,7,4'-THIF was obtained from Chromadex™ (Irvine, CA, USA), and Dulbecco’s modified eagle medium (DMEM) and MMP-1 antibody were purchased from Thermo Fisher Scientific (San José, CA, USA). Medium 199 (M199) and daidzein were purchased from Sigma-Aldrich (St. Louis, MO, USA), and fetal bovine serum (FBS) was purchased from Gemini Bio-Products (Calabasas, CA, USA). CNBr-Sepharose 4B, [γ-32P]-ATP and the chemiluminescence detection kit were obtained from Amersham Pharmacia Biotech (Piscataway, NJ, USA). The protein assay kit was purchased from Bio-Rad Laboratories (Hercules, CA, USA). MTS solution was from Promega (Madison, WI, USA). Penicillin/streptomycin was purchased from Invitrogen (Grand Island, NY, USA). Primary antibodies recognizing phosphorylated MEK (Ser^217/221), total MEK, phosphorylated SEK1/MKK4 (MKK4, Ser²⁵⁷/Thr²⁶¹), phosphorylated MKK3 (Ser¹⁸⁹)/6 (Ser²⁰⁷), total MKK3, phosphorylated p38 (Tyr^180/182), total p38 and PKCα were purchased from Cell Signaling Technology (Danvers, MA). Antibodies against phosphorylated-ERKs (Tyr²⁰⁴), total ERKs, ERK2, total MKK4, phosphorylated JNK (Thr¹⁸³/Tyr¹⁸⁵), total JNK, JNK2 and phosphorylated PKCα (Ser⁶⁵⁷) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Active PKCα and PKCδ proteins were purchased from Millipore (Bedford, MA, USA).

The MTS solution was purchased from Promega (Madison, WI, USA). Dulbecco’s modified Eagle medium (DMEM), fetal bovine serum (FBS), penicillin/streptomycin, and MMP-1 antibody were procured from Thermo Fisher Scientific (San Jose, CA, USA). Primary antibodies recognizing phosphorylated MEK (Ser^217/221), total MEK, phosphorylated SEK1/MKK4 (MKK4, Ser²⁵⁷/Thr²⁶¹), phosphorylated MKK3/6 (Ser¹⁸⁹/Ser²⁰⁷), phosphorylated p44/p42 MAPK (ERK1/2) (Thr²⁰²/Tyr²⁰⁴), phosphorylated SAPK/JNK (Thr¹⁸³/Tyr¹⁸⁵), total MKK3, phosphorylated c-Jun (Ser⁷³), phosphorylated p38 (Tyr^180/182), and total p38 were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies against total ERK, total MKK4, and total JNK were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA).