The following pre-conjugated antibodies were used for flow cytometry: CD56-PE (clone 5.1H11), CD56-PC7 (clone 5.1H11), CD56-PacBlue (clone 5.1H11), CD3-FITC (clone OKT3), CD16-BV786 (clone 3G8), CD57-PE (clone HCD57), CD3-APC (clone OKT3), CD3-PacBlue (clone UCHT1), CD25-PE (clone PC61.5), PD-L1-APC (clone 29E.2A3), CD8-PC7(clone RPA-T8), IFNγ-Alexa647(clone 4S.B3), TNFα-PacBlue(clone Mab11) (BioLegend); CD45-APC(clone 2D1), CD45-eFluor450(clone 2D1), PD-1-eVolve655 (clone J105), FoxP3-FITC (clone PCH101), TNFα-eF450 (clone Mab11) (eBioscience); and CD4-APC-H7 (clone RPA-T4) (BD Biosciences). All samples were acquired on a CytoFlex flow cytometer and analyzed using CyteExpert or FlowJo software (v10.0.7).
Cd8 pc7
Manufactured by BioLegend
CD8-PC7 is a fluorochrome-conjugated antibody that binds to the CD8 protein, which is expressed on the surface of certain T cells. The core function of CD8-PC7 is to enable the identification and analysis of CD8-positive cells in various applications, such as flow cytometry.
Automatically generated - may contain errors
Lab products found in correlation
Cd3 fitc, by BioLegend (2 mentions)
Cd56 pe, by BioLegend (1 mentions)
Cd3 apc, by BioLegend (1 mentions)
Cd45 apc, by BioLegend (1 mentions)
Cd57 pe, by BioLegend (1 mentions)
Cd25 pe, by BioLegend (1 mentions)
Pd l1 apc, by BioLegend (1 mentions)
Cd4 apc h7, by BD (1 mentions)
Cd45 efluor450, by Thermo Fisher Scientific (1 mentions)
Cd16 bv786, by BioLegend (1 mentions)
Cd3 pacblue, by BioLegend (1 mentions)
Ifnγ alexa647, by BioLegend (1 mentions)
Cd16 pe cy7, by BioLegend (1 mentions)
Cd4 pc5, by BioLegend (1 mentions)
Cd3 cf, by Immunostep (1 mentions)
Cd56 pc5, by BioLegend (1 mentions)
Lamp1 apc, by BioLegend (1 mentions)
Gallios flow cytometer, by Beckman Coulter (1 mentions)
2 protocols using cd8 pc7
1
Comprehensive Immune Cell Profiling
2
Multiparameter Flow Cytometry Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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For flow cytometry, cells were washed with PBA [PBS supplemented with 0.5% bovine serum albumin (BSA), 1% FBS and 0.1% sodium azide] and incubated with antibodies against surface markers [CD3-CF (Immunostep); CD3-FITC, CD56-PC5, CD16-PE-Cy7, CD4-PC5.5, CD8-PC7 and LAMP1-APC (Biolegend)] at 4°C for 30 min in the dark. For intracellular staining, after surface labelling, cells were fixed with 1% p-formaldehyde for 10 min at RT, permeabilized with 0.2% saponin for 10 min at RT and stained with Perforin-PE, Granzyme B-PE (Biolegend) for 30 min. Cells were washed in PBA and analyzed using a Gallios Flow Cytometer (Beckman Coulter). Analysis of the experiments was performed using Kaluza software.
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