Cell cultures were grown on 12 mm diameter glass coverslips, fixed in ½ strength Karnovsky’s fixative, dehydrated through an ethanol series, critical point dried with a SamDri-795 critical point dryer (Tousimis) and coated with chromium using an Ion Beam Coater 610 (Gatan). Samples were photographed on a JEOL 7401F Field Emission Scanning Electron Microscope (JEOL).
Ion beam coater 610
Manufactured by Ametek
The Ion Beam Coater 610 is a compact, benchtop instrument designed for thin-film deposition. It utilizes an ion beam to deposit a range of materials, including metals and dielectrics, onto substrates. The core function of the Ion Beam Coater 610 is to create uniform, adherent thin films on various sample types.
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Lab products found in correlation
2 protocols using ion beam coater 610
1
Scanning Electron Microscopy Protocol
2
Histological and Ultrastructural Analysis of Cell Cultures
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Histological assessment was carried out in cell cultures grown on 12‐well Transwell inserts (Corning). The samples were fixed with half strength Karnovsky's fixative, post‐fixed in 2% osmium tetroxide, dehydrated with graded ethyl alcohol solutions and embedded in tEPON‐812 epoxy resin (Tousimis) in a Llynx II EM Tissue Processor (Electron Microscopy Sciences). Semi‐thin (1 μm) sections were cut on a Leica EM UC7 ultramicrotome (Leica Microsystems), stained with toluidine blue and imaged by brightfield microscopy. For scanning electron microscopy, cell cultures were grown on 12 mm diameter glass coverslips, fixed in half strength Karnovsky's fixative, dehydrated through an ethanol series, critical point dried with a SamDri‐795 critical point dryer (Tousimis) and coated with chromium using an Ion Beam Coater 610 (Gatan). Samples were photographed on a JEOL 7401F Field Emission scanning electron microscope (JEOL). The area of cells with unambiguous cell–cell borders was outlined and quantified using ImageJ software (National Institutes of Health).
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