Rig 1 d14g6

Manufactured by Cell Signaling Technology

Sourced in United States, United Kingdom

RIG-I (D14G6) is a primary antibody that recognizes the RIG-I (Retinoic acid-inducible gene I) protein. RIG-I is a cytosolic pattern recognition receptor that plays a crucial role in the detection of viral RNA and the induction of the innate immune response. This antibody is suitable for use in various applications, such as Western blotting, immunoprecipitation, and immunohistochemistry, to study the expression and localization of the RIG-I protein.

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Rabbit anti-RIG-I (D14G6) Anti-RIG-I (D14G6, RIG-I

3 protocols using rig 1 d14g6

Immunoblotting Analysis of Innate Immune Signaling

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Lysates for immunoblotting were prepared from isolated NB cells after treatment with 50 μg/mL of polyinosinic‐polycytidylic acid high molecular weight [poly (I:C)HMW; Invitrogen] or polyinosinic‐polycytidylic acid low molecular weight [poly (I:C)LMW; Invitrogen]. Total protein was extracted by PRO‐PREPTM Protein Extraction Solution (iNtRON Biotechnology; Seoul, Korea). Equal amounts of protein (30 μg) were separated on a 10%‐15% SDS–PAGE and transferred to nitrocellulose membranes. Immunoblotting was performed using antibodies to LGP2 (Proteintech Group), MDA5 (D74E4; Cell Signaling Technology, Danvers, MA, USA), RIG‐I (D14G6; Cell Signaling Technology), TLR3 (Abcam, Cambridge, MA, USA), MAVS (Santa Cruz Biotechnology, Dallas, TX, USA), Bcl‐2 (50E3; Cell Signaling Technology), t‐Bid (Cell Signaling Technology), Noxa (Abcam), β‐actin (Millipole, Billerica, MA, USA) and GAPDH (GeneTex, Irvine, CA, USA). After washing 3 times, membranes were incubated with HRP‐conjugated anti‐mouse (Jackson ImmunoResearch Europe, Newmarket, Suffolk, UK) and anti‐rabbit (Jackson ImmunoResearch) secondary antibody for 60 minutes at room temperature. Immunoblotting analysis was performed as described previously.13

Lin L., Huang C., Wu M., Hsu W, & Chuang J. (2018). Innate immune sensor laboratory of genetics and physiology 2 suppresses tumor cell growth and functions as a prognostic marker in neuroblastoma. Cancer Science, 109(11), 3494-3502.

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Murine Norovirus Protein Detection

Cited 2 times

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DMXAA, H151 and 2ʹ3ʹ-cGAMP were purchased from InvivoGen. JAK inhibitor 1 (SC-204021) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). N-Acetyl-L-cysteine was purchased from Sigma-Aldrich. Rabbit polyclonal antisera to MNV NS1/2 was kindly provided by Prof. Vernon K. Ward (School of Biomedical Sciences, University of Otago, New Zealand).^{47 (link)} Rabbit polyclonal antisera to MNV NS7 was kindly provided by Prof. Ian Goodfellow (Department of Pathology, University of Cambridge, UK).^{48 (link)} Antibodies against STAT1 (#9172), pSTAT1 (Ser727, #9177), RIG-I (D14G6, #3743), MDA5 (D74E4, #5321), cGAS (D3O8O, #31659), STING (D2P2F, #13647), Myc-tag (71D10, #2278) and Myc-Tag (9B11, #2276) were purchased from Cell Signaling Technology. Rabbit anti-GBP2 (11854-1-AP) and anti-GBP5 (13220-1-AP) antibodies were purchased from Proteintech. Mouse anti-Flag (F1804, Sigma-Aldrich) and anti-β-actin (#sc-47778, Santa Cruz Biotechnology) antibodies were used. Anti-rabbit and anti-mouse IRDye-conjugated secondary antibodies (Li-Cor Bioscience, Lincoln, USA) were used.

Yu P., Miao Z., Li Y., Bansal R., Peppelenbosch M.P, & Pan Q. (2021). cGAS-STING effectively restricts murine norovirus infection but antagonizes the antiviral action of N-terminus of RIG-I in mouse macrophages. Gut Microbes, 13(1), 1959839.

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Comprehensive Antibody Protocol for Cytometry and Western Blotting

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Flow cytometry antibodies are listed in Supplementary Table 1 and
immunohistochemistry antibodies are listed above. For western blotting, primary
antibodies against ADAR1 (15.8.6, Santa Cruz Biotechnology), PKR (EPR19374
Abcam), RIG-I (D14G6, Cell Signaling Technology), MDA5 (D74E4, Cell Signaling
Technology), STAT1 (p91, Polyclonal Goat IgG, R&D Systems), and MAVS (Rabbit
Polyclonal IgG, ThermoFisher Scientific)were used. Peroxidase-conjugated
secondary antibodies against rabbit IgG, mouse IgG or goat IgG were purchased
from Jackson Laboratories. IRDye secondary antibodies against rabbit IgG, mouse
IgG or goat IgG were purchased from LI-COR Biosciences.

Ishizuka J.J., Manguso R.T., Cheruiyot C.K., Bi K., Panda A., Vellve A.I., Miller B.C., Du P.P., Yates K.B., Dubrot J., Buchumenski I., Comstock D.E., Brown F.D., Ayer A., Kohnle I.C., Pope H.W., Zimmer M.D., Sen D.R., Lane-Reticker S.K., Robitschek E.J., Griffin G.K., Collins N.B., Long A.H., Doench J.G., Kozono D., Levanon E.Y, & Haining W.N. (2018). Loss of ADAR1 in tumours overcomes resistance to immune checkpoint blockade. Nature, 565(7737), 43-48.

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