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2 protocols using ofloxacin

1

Antimicrobial Screening of Natural Compounds

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CBD (98%, HPLC purity) was purchased from Guilin Layn Natural Ingredients Corp., Guangxi, China. Olivetol (98%, HPLC purity) was purchased from Shenghong Corporation, Shanghai, China. α-Phellandrene (85%, HPLC purity) was purchased from Sigma-Aldrich, Shanghai, China. BF3·2H2O (AR, 99%), CuCl2·2H2O (AR, 97%), AlCl3 (AR, 99%), and FeCl3 (AR, 98%) were purchased from Macklin Biochemical Co., Ltd., Shanghai, China. All other chemicals were purchased from Sinopharm Chemical Reagent Co., Ltd., Shanghai, China. All the chemicals were of analytical grade and used as received unless otherwise stated.
Escherichia coli (CICC 20549) and Staphylococcus aureus (CICC 23656) strains were supplied by the China Center of Industrial Culture Collection (CICC), Beijing, China. Tetracycline hydrochloride, gentamicin, chloramphenicol, and ofloxacin were obtained from Sinopharm Chemical Reagent Co., Ltd., Shanghai, China.
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2

Comprehensive Analytical Reagent Protocol

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Beta-cyclodextrin (β-CD), potassium hydroxide (KOH), absolute ethanol (C 2 H 5 OH), chloroplatinic acid (H 2 PtCl 4 ), KB, potassium ferricyanide (K 3 [Fe(CN) 6 ]), potassium chloride (KCl), sodium chloride (NaCl), ofloxacin, glucose, ascorbic acid (AA), and uric acid (UA) were obtained from Sinopharm Chemical Reagent Co. Ltd (Beijing, China, https://www.sigmaaldrich. com/china-mainland.html). Fetal bovine serum (FBS, #10439024) was purchased from Thermo Fisher Scientific (https://www.thermofisher.com/cn/zh/home.html), and it was stored at -20 °C before use. All chemicals were of analytical grade without further treatment. Nanopure deionized and distilled water (DDW, 18.25 MΩ cm) was used in all the experiments.
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