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Anti β actin and tubulin

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Anti-β actin and tubulin are antibodies used to detect the presence and abundance of β-actin and tubulin proteins in biological samples. These proteins are widely expressed structural components found in the cytoskeleton of eukaryotic cells. The antibodies can be used in various analytical techniques, such as Western blotting, immunocytochemistry, and immunohistochemistry, to study the expression and localization of these proteins.

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Lab products found in correlation

Anti map2, by Abcam (2 mentions) Anti synj1, by Thermo Fisher Scientific (2 mentions) Anti ptau at8 and tau 5, by Thermo Fisher Scientific (2 mentions) Anti holoapp mab348 and 6e10, by Merck Group (2 mentions) Anti beta amyloid, by Cell Signaling Technology (2 mentions) Texas red and alexa555 conjugated anti mouse and rabbit igg, by Thermo Fisher Scientific (2 mentions) Mir 195 inhibitors, by Applied Biological Materials (2 mentions) Am00100, by Applied Biological Materials (2 mentions) Amm1017200, by Applied Biological Materials (2 mentions) Amm3026700, by Applied Biological Materials (2 mentions) Mirna extraction kit and qpcr probes for specific mirnas, by Qiagen (2 mentions) Anti rab5, by Santa Cruz Biotechnology (2 mentions) Mab348, by Merck Group (1 mentions) Texas red, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

β-actin (5071 citations) Anti-β-actin (1828 citations) Anti-actin (514 citations) β-tubulin (236 citations) Anti-tubulin (154 citations) Anti-β-tubulin (135 citations)

3 protocols using anti β actin and tubulin

1

Investigating Synj1 and Tau Proteins

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The anti-synj1 (rabbit polyclonal Ab, Novus, RRID: AB_11047653), anti-pTau AT8 and Tau-5 (ThermoFisher, RRID: AB_223647 and 10980631), anti-Rab5 (Santa Cruz Biotechnology, RRID:AB_628191), anti-β actin and tubulin (Santa Cruz Biotechnology, RRID:AB_476697 and 477498), anti-holoAPP MAB348 and 6E10 (Millipore RRID:AB_94882 and 564201), anti-beta-Amyloid (Cell Signaling Technology, RRID: AB_2056585), anti-MAP2 (Abcam, RRID:AB_297885), anti-dynamin clone 41 for (BD bioscience; RRID:AB_3976413), anti-mouse and rabbit HRP (ThermoFisher, RRID:AB_2556542 and 2540618), Texas-Red and Alexa555 conjugated anti-mouse and rabbit IgG (ThermoFisher, RRID:AB_10374713, 10983944, 2535987 and 1090271) were purchased. AAV2-containing miR-195, miR-374, scramble controls and miR-195 inhibitors were generated and obtained from ABM Inc. with detailed sequence information available (Am00100, Amm1017200 and Amm3026700). The miRNA extraction kit and qPCR probes for specific miRNAs were purchased from Exiqon Inc. The qPCR probes for actin (Hs1060665_g1), synj1 (Hs00953234_m1 and Mm01210539_m1), gapdh (Mm99999915_g1), RNU6B (NR_002752), 18 s and 45 s (4331182, Mm03928990_g1) were also purchased from ThermoFisher.
Cao J., Huang M., Guo L., Zhu L., Hou J., Zhang L., Pero A., Ng S., El Gaamouch F., Elder G., Sano M., Goate A., TCW J., Haroutunian V., Zhang B, & Cai D. (2020). MicroRNA-195 rescues ApoE4-induced cognitive deficits and lysosomal defects in Alzheimer’s disease pathogenesis. Molecular Psychiatry, 26(9), 4687-4701.
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2

Antibody Validation for Neurodegenerative Markers

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The following commercially available antibodies were used: anti-pTau (AT8; pSer202, pThr205) and total Tau (Tau 5) from ThermoFisher (RRID:AB_223647 and 10980631), anti-synj1 (rabbit polyclonal Ab, Novus; RRID:AB_11047653), anti-β actin and tubulin (Santa Cruz; RRID:AB_476697 and 477498), anti-holoAPP (MAB348, Millipore; RRID:AB_94882), anti-GSK3β (rabbit monoclonal Ab, Cell Signaling; RRID:AB_490890), anti-pGSK3β (Ser9, inactive form of GSK3β, rabbit polyclonal Ab, Cell Signaling; RRID:AB_331405), anti-pGSK3β (Y216, active form of GSK3β, rabbit polyclonal Ab, Abcam; RRID:AB_1310290), anti-mouse and rabbit horse radish peroxidase, Texas-Red or Alexa488 conjugated anti-mouse IgG (ThermoFisher; RRID:AB_2556542, 2540618, 10374713, 10983944, 2535987 and 1090271), were purchased. The pAb369 antibody which recognizes the C-terminus of APP was used to detect mouse holo-APP and c-terminal fragments67 (link).
Cao J., Gaamouch F.E., Meabon J.S., Meeker K.D., Zhu L., Zhong M.B., Bendik J., Elder G., Jing P., Xia J., Luo W., Cook D.G, & Cai D. (2017). ApoE4-associated phospholipid dysregulation contributes to development of Tau hyper-phosphorylation after traumatic brain injury. Scientific Reports, 7, 11372.
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3

Detailed Protocol for Analyzing Synaptic Proteins

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The anti-synj1 (rabbit polyclonal Ab, Novus, RRID: AB_11047653), anti-pTau AT8 and Tau-5 (ThermoFisher, RRID: AB_223647 and 10980631), anti-Rab5 (Santa Cruz Biotechnology, RRID:AB_628191), anti-β actin and tubulin (Santa Cruz Biotechnology, RRID:AB_476697 and 477498), anti-holoAPP MAB348 and 6E10 (Millipore RRID:AB_94882 and 564201), anti-beta-Amyloid (Cell Signaling Technology, RRID: AB_2056585), anti-MAP2 (Abcam, RRID:AB_297885), anti-dynamin clone 41 for (BD bioscience; RRID:AB_3976413), anti-mouse and rabbit HRP (ThermoFisher, RRID:AB_2556542 and 2540618), Texas-Red and Alexa555 conjugated anti-mouse and rabbit IgG (ThermoFisher, RRID:AB_10374713, 10983944, 2535987 and 1090271) were purchased. AAV2-containing miR-195, miR-374, scramble controls and miR-195 inhibitors were generated and obtained from ABM Inc. with detailed sequence information available (Am00100, Amm1017200 and Amm3026700). The miRNA extraction kit and qPCR probes for specific miRNAs were purchased from Exiqon Inc. The qPCR probes for actin (Hs1060665_g1), synj1 (Hs00953234_m1 and Mm01210539_m1), gapdh (Mm99999915_g1), RNU6B (NR_002752), 18s and 45s (4331182, Mm03928990_g1) were also purchased from ThermoFisher.
Cao J., Huang M., Guo L., Zhu L., Hou J., Zhang L., Pero A., Ng S., El Gaamouch F., Elder G., Sano M., Goate A., TCW J., Haroutunian V., Zhang B, & Cai D. (2020). MicroRNA-195 rescues ApoE4-induced cognitive deficits and lysosomal defects in Alzheimer’s disease pathogenesis. Molecular Psychiatry, 26(9), 4687-4701.
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