The following conjugated antibodies were used for cell-surface staining: CD3-Pacific Blue (BD Pharmingen; Clone UCHT1; Cat. No. 558117; San Diego, CA, USA), CD8-APC H7 (BD Pharmingen; Clone SK1; Cat. No. 641400), CD45RA-PE (BD Pharmingen; Clone HI100; Cat. No. 555489), CCR7-PE-Cy7 (BD Pharmingen; Clone 3D12; Cat. No. 557648), CD28-PerCP-Cy5.5 (BD Biosciences; Clone L293; Cat. No. 337181; San Jose, CA, USA), CD27-Alexa Fluor 700 (BD Pharmingen; Clone M-T271; Cat. No. 560611), CD95-APC (BD Pharmingen; Clone DX2; Cat. No. 558814) and CD127-FITC (BD Pharmingen; Clone HIL-7R-M21; Cat. No. 560549). Conjugated antibodies for intracellular staining included the following: IFN-γ-FITC (BD Pharmingen; Clone 4S.B3; Cat. No. 554551), IL-2-PerCP-Cy5.5 (BD Pharmingen; Clone MQ1-17H12; Cat. No. 560708) and TNF-α-AlexaFluor 700 (BD Pharmingen; Clone MAb11; Cat. No. 557996). To exclude dead cells, the Fixable Aqua Dead Cell Stain viability marker was used (Invitrogen; Cat. No. L34957; Eugene, OR, USA).
Clone mq1 17h12
Manufactured by BD
The Clone MQ1-17H12 is a laboratory equipment product. It serves a core function, but a detailed description while maintaining an unbiased and factual approach is not available.
Automatically generated - may contain errors
Lab products found in correlation
Ccr7 pe cy7, by BD (1 mentions)
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Clone hil 7r m21, by BD (1 mentions)
Clone 4s b3, by BD (1 mentions)
Tnf α alexafluor 700, by BD (1 mentions)
Cd27 alexa fluor700, by BD (1 mentions)
Clone mab11, by BD (1 mentions)
Clone ucht1, by BD (1 mentions)
Il 2 percp cy5, by BD (1 mentions)
Cd95 apc, by BD (1 mentions)
Cd8 apc h7, by BD (1 mentions)
Cd3 pacific blue, by BD (1 mentions)
Cd127 fitc, by BD (1 mentions)
Cd28 percp cy5, by BD (1 mentions)
Clone jes3 9d7, by BD (1 mentions)
2 protocols using clone mq1 17h12
1
Comprehensive Immune Cell Profiling
2
Intracellular Cytokine Detection in Whole Blood
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The ability of the study's assay to detect intracellular cytokines in T cells and monocytes in whole blood samples was assessed with particular interest focused on the following cytokines, all PE-conjugated: TNF-α (BD FastImmune, Clone 6401.111), IFN-γ (BD FastImmune, Clone 25723.11), IL-10 (BD Pharmingen, Clone JES3-9D7), IL-6 (BD Pharmingen, Clone MQ2-13A5), IL-4 (BD FastImmune, Clone 3010.211), IL-2 (BD Pharmingen, Clone MQ1-17H12), TGF-β (BD Pharmingen, Clone TW4-2F8), IL-12 p40 (BD Pharmingen, Clone C11.5) and IL-12 p70 (BD Pharmingen, Clone 20C2). Stimulation was performed as described earlier and the antibody labelling was performed using 50 μl of whole blood as already described with anti-CD3-PerCP used at a ratio of 1:25, anti-CD14-APC at a ratio of 1:50 and anti-cytokine-PE at a ratio of 1:12.5. One tube was included with unstimulated blood and labelled with anti-TNF-α−PE at the same ratio to account for any background noise and another tube was included with stimulated blood but labelled with an isotype control at the same ratio.
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