Scientz iid ultrasonic homogenizer

Manufactured by Ningbo Scientz Biotechnology

Sourced in China

The Scientz-IID Ultrasonic Homogenizer is a laboratory instrument designed to homogenize samples through the application of high-frequency ultrasonic waves. The device generates powerful ultrasonic vibrations that disrupt cellular structures and thoroughly mix the contents of the sample.

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Lab products found in correlation

Ni nta agarose, by GE Healthcare (1 mentions) Falcon cell strainer, by BD (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) Ovalbumin (ova), by InvivoGen (1 mentions) Pierce protein a g agarose, by Thermo Fisher Scientific (1 mentions) Mouse il 15 elisa kit, by Neobioscience (1 mentions) Atp solution, by Thermo Fisher Scientific (1 mentions) Red blood cell lysis buffer, by Solarbio (1 mentions) Sulfo cy7 nhs ester, by Lumiprobe (1 mentions) Bca assay kit, by Solarbio (1 mentions)

Spelling variants of this product

Scientz-IID (60 citations) Ultrasonic Homogenizer (14 citations)

7 protocols using scientz iid ultrasonic homogenizer

Colloidal Curcumin Delivery via Oleobases

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Cur was loaded into OBs via ultrasonic treatment combined with pH-driven methods to produce a colloidal delivery system [2] . The pure OB system was obtained using a Scientz-IID ultrasonic homogenizer (NingBo Scientz Biotechnology Co., Ltd., Ningbo, China) at different ultrasonic power (0–250 W) for 10 min (2 s on/ 4 s off) at room temperature in the dark. An ultrasonic probe (6.36 mm) was placed 1 cm from the bottom and operated at a frequency of 20 kHz. Cur alkaline solution (5 mg/g) was added to the OBs (1:10, w/w), and the pH was immediately adjusted to 7.0. Finally, the samples were centrifuged to collect the supernatant yellow layer to obtain Cur-loaded OBs.
OB emulsions were prepared by dispersing 10 g of Cur-loaded OB samples (wet weight) in 90 g of DI water, followed by homogenization at 4000 rpm for 5 min.

Zhu J., Wang H., Miao L., Chen N., Zhang Q., Wang Z., Xie F., Qi B, & Jiang L. (2023). Curcumin-loaded oil body emulsions prepared by an ultrasonic and pH-driven method: Fundamental properties, stability, and digestion characteristics. Ultrasonics Sonochemistry, 101, 106711.

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Recombinant Expression and Purification of DyP Peroxidase

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The genomic DNA of C2 was used as a template for gene-specific primed PCR amplification of the DyP gene. The gene encoding DyP was cloned into pET-28a(+), and the recombinant plasmid pET28(+)-DyP was transformed into E. coli BL21(DE3). The expression of DyP was induced by the addition of 0.2 mM IPTG when the optical density reached approximately 0.6 at 600 nm. The cells were collected by centrifugation at 6000 rpm and 4°C for 30 min and resuspended in 5 mL of phosphate-buffered saline (PBS) at pH 7.2. The suspension was subjected to ultrasonication with a SCIENTZ-IID ultrasonic homogenizer (Ningbo Scientz Biotechnology Co., Ltd. Zhejiang Province, China). The resulting cell lysate was centrifuged at 4°C for 30 min at 12,000 rpm. Then, SDS–PAGE was performed to analyze the supernatant. The protein was purified with Ni-NTA agarose (GE Healthcare, United States) according to the methods described by Zang et al. (2020) (link). The molecular mass of the DyP protein was determined by SDS–PAGE. The purified DyP protein concentration was determined by the Bradford method (Zang et al., 2020 (link)).

Jiang C., Yan H., Shen X., Zhang Y., Wang Y., Sun S., Jiang H., Zang H., Zhao X., Hou N., Li Z., Wang L., Wang H, & Li C. (2022). Genome Functional Analysis of the Psychrotrophic Lignin-Degrading Bacterium Arthrobacter sp. C2 and the Role of DyP in Catalyzing Lignin Degradation. Frontiers in Microbiology, 13, 921549.

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Dual-Loaded Multilamellar Vesicles Preparation

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A double-emulsion procedure (Li et al., 2019 (link)) was used to prepare MVLs containing ioversol and doxorubicin hydrochloride. Briefly, 1 mL of chloroform containing the lipids (41 mg HSPC:40.5 mg cholesterol:5 mg DPPG: 11.25 mg triolein) in 1 mL aqueous solution (the first aqueous solution) was emulsified by Scientz-IID Ultrasonic Homogenizer (Ningbo Scientz Biotechnology Co., Ltd. Ningbo, China) for 30 sec (30% power output, 25 °C) to produce a w/o emulsion. The first aqueous solution contains 1 mg of doxorubicin hydrochloride in 1 mL of 320 mg ioversol injection. This w/o emulsion (2 mL) was subsequently emulsified with 6 mL of the second aqueous solution containing 4% glucose (wt/vol) and 20 mM lysine at 2800 r/min by XHF-D mixer (Ningbo Scientz Biotechnology Co., Ltd. Ningbo, China) at 40 °C to prepare w/o/w emulsion. Then the w/o/w emulsion was diluted with 4 mL of second aqueous solution poured into 100-mL egg type flask. Chloroform was removed by flushing nitrogen over the surface of the double emulsion at 35–37 °C. The resultant MVLs were collected at 100g for 10 min, and resuspended in sterile saline solution after discarding the supernatant. BD Falcon™ cell strainers (BD Biosciences, USA) were used to isolate different size MVLs, improve the uniformity of MVLs. The ioversol and doxorubicin hydrochloride concentration in MVLs were determined by HPLC.

Tang H., Cao C., Zhang G, & Sun Z. (2023). Impact of particle size of multivesicular liposomes on the embolic and therapeutic effects in rabbit VX2 liver tumor. Drug Delivery, 30(1), 1-16.

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Recombinant IL-15 and IL-15Rα Protein Production

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The IL-15 and IL-15Rα encoded rAD was constructed by Shanghai Genechem Co., Ltd, China. Recombinant mouse IL-15 was provided by Abcam Inc., USA. Pierce^TM Protein A/G Agarose and ATP solution were obtained from ThermoFisher Scientific, USA. Red blood cell lysis buffer was supplied by Beijing Solarbio Science & Technology Co., Ltd, China. Mouse IL-15 ELISA kit was purchased from Neobioscience Technology Co, Ltd, China. Mouse IFN-γ ELISA kit was obtained from Shanghai Jianglai Industrial Limited by Share Ltd, China. OVA was supplied by InvivoGen, USA. Sulfo-Cy7 NHS ester was obtained from Lumiprobe Corporation, USA. LPS was bought from Sigma-Aldrich, USA. Cell culture flasks, plates, and glass bottom culture dishes were purchased from Wuxi NEST Biotechnology Co., Ltd, China. Scientz-IID Ultrasonic Homogenizer and Gene Electroporator Scientz-2C were provided by Ningbo Scientz Biotechnology Co., Ltd, China. All used reagents and solvents were of analytical standard grade unless stated otherwise.

Wang K., Zhang X., Ye H., Wang X., Fan Z., Lu Q., Li S., Zhao J., Zheng S., He Z., Ni Q., Chen X, & Sun J. (2023). Biomimetic nanovaccine-mediated multivalent IL-15 self-transpresentation (MIST) for potent and safe cancer immunotherapy. Nature Communications, 14, 6748.

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Cancer Cell Membrane-Coated Mesoporous Silica Nanoparticles

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Cancer cell membrane–coated MSNs@ICG (CMI) was prepared according to the previous report (Fang et al., 2020 (link)). In brief, the human cervical cancer cells, Hela, were maintained in DMEM supplemented with 10% FBS, 100 U/mL penicillin G, and 100 μg/ml streptomycin. And the cells were cultured at 37°C in a humidified atmosphere of 5% CO₂. The cancer cell membrane (CCM) was extracted from human cervical cancer cells Hela by using a membrane protein extraction kit, following the instructions from the manufacturer (Biyuntian, China). The CCM was added to the MI dispersion, and the mixture was ultrasonically dispersed by a Scientz-IID ultrasonic homogenizer (Ningbo Scientz Biotechnology Co., Ltd., China) for 1 h. Afterward, CMI was extruded by a mini-extruder (Avanti, Canada) through the 100-nm polycarbonate membrane 20 times in the dark. The CCM coating of MSN@DiD (CCM/MSNs@DiD, CMD) was also prepared by the same procedure mentioned before.

Huang C., Guan X., Lin H., Liang L., Miao Y., Wu Y., Bao H., Wu X., Shen A., Wei M, & Huang J. (2021). Efficient Photoacoustic Imaging With Biomimetic Mesoporous Silica-Based Nanoparticles. Frontiers in Bioengineering and Biotechnology, 9, 762956.

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Extraction and Coating of Red Blood Cell Membrane Nanoparticles

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To obtain the fragment of the red blood cell membrane (RBCM), RBCs from SD rats were carefully washed by 1 × PBS three times. After PBS was removed, the RBCs were incubated in 0.25 × PBS (the hypotonic medium) at 4 °C for 3 h. Then, the fully cracked RBCs were centrifuged (10000 × g, 10 min) and repeatedly washed with ice-cold water three times. The collected RBCM was resuspended and sonicated by probe sonicator (Scientz-IID Ultrasonic Homogenizer, Ningbo Scientz Biotechnology Co., Ltd, China) for 5 min, and then freeze-dried and kept at −80 °C for the following experiments.
To coat the Ce6@DiR NPs, RBCM were redissolved and sonicated (100W, 10min) and then mixed with Ce6@DiR NPs at a different mass ratio. The appropriate ratio was selected by Dynamic light scattering. The selected mixture was then extruded through a series of water-phase filters (400 nm, 200 nm, and 100 nm) for at least 10 cycles. The obtained Ce6@DiR-M NPs were kept at 4 °C. The colloid stability of Ce6@DiR-M NPs was further studied in PBS with 10% fetal bovine serum (FBS).
To prepare the PEGylated Ce6@DiR NPs, DSPE-PEG_2K (20 wt%) dissolved in ethanol (5mg mL^-1) was added to the Ce6@DiR NPs before the organic solution was evaporated.

Zhang X., Xiong J., Wang K., Yu H., Sun B., Ye H., Zhao Z., Wang N., Wang Y., Zhang S., Zhao W., Zhang H., He Z., Luo C, & Sun J. (2021). Erythrocyte membrane-camouflaged carrier-free nanoassembly of FRET photosensitizer pairs with high therapeutic efficiency and high security for programmed cancer synergistic phototherapy. Bioactive Materials, 6(8), 2291-2302.

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Cellular Uptake of NAR Nanocarriers

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To study the cellular uptake of NAR NCs, Caco-2 cells were seeded in 24-well plates at a density of 1 × 10⁴ cells/well and further cultured for 14 d. On the 15th d, the cells were washed 3 times with PBS and incubated with 50 µg/ml of NC_F68, NC_F127, NC_HPMC, and NC_PVP, respectively. After 15 min of incubation, the uptake process was stopped and the attached NAR was completely removed by washing the cells 3 times with PBS. Next, the cells were collected and disrupted using a SCIENTZ-IID ultrasonic homogenizer (Ningbo Scientz Biotechnology Co., Ltd., China).
To detect the total content of NAR, 100 µl of samples were treated with β-glucuronidase (20 µl) at 37 °C for 2 h. After incubation, methanol (300 µl) was added and vortexed for 3 min to precipitate proteins. The mixtures were then centrifuged at 10 000 g for 5 min. The supernatant was analyzed using the high-performance liquid chromatography (HPLC) as described in Supplementary materials. The total protein was quantified using a BCA assay kit (Beijing Solarbio Science & Technology Co., Ltd., China).

Zhang G., Guan H., Li J., Li M., Sui X., Tian B., Dong H., Liu B., He Z., Li N., Zhao M, & Fu Q. (2022). Roles of effective stabilizers in improving oral bioavailability of naringenin nanocrystals: Maintenance of supersaturation generated upon dissolution by inhibition of drug dimerization. Asian Journal of Pharmaceutical Sciences, 17(5), 741-750.

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