TiNTs were prepared by a classical hydrothermal method and characterized as reported previously.18 (link) Surface charge was determined by Malvern Zetasizer 2000 (Zetasizer Nano ZS90, Malvern Instruments Ltd, UK). Before measurement, the freshly prepared TiNTs were appropriately suspended in pure water (0.1 mg mL−1) then ultrasonicated during 5 min. Then a sample of TiNTs suspension was observed by TEM using transmission electron microscope Tecnai G2 at 200 kV (FEI, Netherland) and acquire with a Veleta camera (Olympus, Japan). For crystalline phase X-ray powder diffraction was performed at room temperature with an X-ray diffractometer (X' Pert PRO MPD, PANalytical Co., Holland). Monochromatic Cu Kα-radiation (λ = 1.5418 Å) was obtained with a Ni-filtration and a system of diverging and receiving slides of 0.5° and 0.1 mm respectively. The diffraction pattern was measured with a voltage of 40 kV and a current of 30 mA over a 2θ range of 3–40° using a step size of 0.02° at a scan speed of 1 s per step.
Zetasizer 2000
Manufactured by Malvern Panalytical
Sourced in United Kingdom
The Zetasizer 2000 is a lab equipment product from Malvern Panalytical that measures the size, size distribution, and zeta potential of small particles and molecules in suspension or solution.
Automatically generated - may contain errors
Lab products found in correlation
Jem 200ex, by Hitachi (4 mentions)
Mastersizer 2000, by Malvern Panalytical (2 mentions)
Tecnai g2 f20, by Thermo Fisher Scientific (2 mentions)
Supra 55vp, by Zeiss (2 mentions)
Tecnai g2, by Thermo Fisher Scientific (1 mentions)
Zetasizer nano zs90, by Malvern Panalytical (1 mentions)
Veleta camera, by Olympus (1 mentions)
Cary 50 bio uv visible spectrophotometer, by Agilent Technologies (1 mentions)
Prism 8, by GraphPad (1 mentions)
Zetasizer lab, by Malvern Panalytical (1 mentions)
Zetasizer ht, by Malvern Panalytical (1 mentions)
Nio nps, by JEOL (1 mentions)
Jem 2100f, by JEOL (1 mentions)
Jem 100cx 2, by JEOL (1 mentions)
Quanta 250 esem, by Thermo Fisher Scientific (1 mentions)
Mastersizer micro, by Malvern Panalytical (1 mentions)
Ftir instrument, by PerkinElmer (1 mentions)
Xrd 6000, by Shimadzu (1 mentions)
Jsm 6610lv scanning electron microscope, by JEOL (1 mentions)
Jec 530, by JEOL (1 mentions)
62 protocols using zetasizer 2000
1
Characterization of Titanate Nanotubes
2
Characterization of Tadalafil-Loaded Niosomes
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The surface potential, vesicle size, and polydispersity index (PDI) of tadalafil-loaded niosomes were measured at 25 ± 2 °C and 90° scattering angle by Zeta sizer 2000 (Malvern Instruments, England, UK) [15 (link)]. The above-mentioned characteristics were measured to aid further in the optimization of the preparation. All measurements were carried out in triplicate, the mean and the standard deviation were computed.
3
Flocculation of Chlorella Induced by SPM
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Based on the results of the PCA analysis, SPM from the Paihua site was used in the algal flocculation test. The experiments were performed in a jar test apparatus (ZR3-6, Zhongrun Water Industry Technology Development Co. Ltd., China) with a series of 300-ml beakers containing 200 ml of Chlorella pyrenoidosa cultures in mid exponential growth phase. Initial Chlorella pyrenoidosa abundance was 4.5 × 106 cells/mL. After addition of the SPM (at 30, 50, 90, and 150 mg/L, representing the concentrations of SPM in the Paihua site during the discharging period throughout the field measurement), the solution was stirred at 200 rpm for 1 min and at 40 rpm for another 15 min. The control was run in the above-mentioned algae media without the addition of SPM. After sedimentation for 30 min, samples were collected from 5 cm below the surface for flocs size determination. All flocculation experiments were conducted in triplicate, the results are presented as mean values and standard deviations. Surface charge of SPM was characterized using a Zetasizer 2000 (Malvern Co. United Kingdom). Algal floc size was quantitatively assessed using a laser particle size analyzer Mastersizer 2000 (Malvern Co. United Kingdom) and denoted by the measured mean diameter (D0.5)31 (link).
4
Cubosomal Dispersion Stability Evaluation
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To confirm the physical stability of the prepared TQ-loaded self-assembled cubosomal dispersion, zeta potential (ZP) was evaluated utilizing Zeta sizer 2000 (Malvern instruments, UK), where values were determined through the dispersion technology software. Prior to measurement of each dispersion, sample dilution using Milli Q water was carried out. All determinations were performed in triplicate.31 (link)
5
Physicochemical Characterization of Nanomaterials
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Dynamic laser scattering (DLS) measurements were performed using a laser light scattering spectrometer (BI-200SM) equipped with a digital correlator (BI-9000AT) at 532 nm at RT. Transmission electron microscopy (TEM) measurements were conducted using a JEM-2100 electron microscope at an accelerating voltage of 200 kV; a small drop of solution was deposited onto a carbon-coated copper EM grid and dried at RT under atmospheric pressure. The UV–vis spectra were recorded on a Cary 50 Bio UV–Visible Spectrophotometer (Varian, USA) equipped with two silicon diode detectors and a xenon flash lamp. Zeta-potentials were measured using a temperature-controlled Zetasizer 2000 (Malvern Instruments. Ltd. UK).
6
Vesicle Size and Zeta-Potential Analysis
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The size and size distribution [27] and zeta-potential [28] (link) of vesicles were measured using a Malvern Zetasizer 2000 instrument (Malvern Instruments, Malvern, UK). Samples were diluted to a total lipid concentration of approximately 0.25 mg/ml in 20 mM HEPES/HCl, pH 7.4 prior to measurements. Zetasizer measures distribution of size in a population and the sizes provided in Tables 1 –3 reflect peaks of this distribution in nm. Each peak is characterized by a width at its base (in nm), which reflects homogeneity (or polydispersity) of the subpopulation under this peak. Each sample was measured five times and the result is expressed as an average value ± standard deviation.
7
Bilosomal Vesicle Characterization
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A Zetasizer 2000 (Malvern Instruments Ltd., Malvern, UK) was utilized to estimate BER-CTS-BLS particle diameter and surface charge using dynamic light scattering [39 (link)]. The formulated bilosomal vesicles were diluted with PBS before being used in the study. Three sets of measurements were made, and the mean results were reported.
8
Evaluating CaF2:Ce,Gd,Nd NPs Stability
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To assess the stability of CaF2: Ce, Gd, Nd NPs in physiologically relevant buffers, we dissolved CaF2: Ce, Gd, Nd NPs at a concentration of 0.2 mg/mL in 50% FCS solution. The samples were kept in a shaker at 37 °C, and we used a Malvern ZetaSizer 2000 (Malvern, UK) to measure the size and zeta potential at 0 h, 1 d, 2 d, 3 d, 4 d, 5 d, 6 d and 7 d. Data analysis used Zetasizer Software (Version 7.13) and GraphPad Prism 8.
9
Nanoparticle Size and Zeta Analysis
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NPs samples were run on a NanoSight NS500. All samples were diluted to a concentration between 1 × 108 and 5 × 108 particles per mL in deionized H2O. Five 60-s videos were taken of each sample to capture particles movement. The NanoSight software tracked the particles individually and using the Stokes–Einstein equation, calculated the hydrodynamic diameters. The zeta potential of NPs was measure in PBS (pH 7.4) using a Malvern Zeta Sizer 2000 (Malvern Instruments).
10
Particle Size Analysis of NPs
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The developed NPs were washed with double distilled water (filtered through 0.22 μ) several times before particle size analysis. Samples were analyzed using Zeta Sizer 2000 (Malvern instrument, UK) which allows sample measurement in the range of 0.02–2000.00 μm and particle size distribution.
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