Log In Sign up
The largest database of trusted experimental protocols

Gfp filter cube

Manufactured by Leica
Visit Supplier

The GFP filter cube is an optical filter designed for use in fluorescence microscopy. It is specifically engineered to isolate the fluorescence signal of green fluorescent protein (GFP) molecules, allowing researchers to visualize and study GFP-labeled samples.

Automatically generated - may contain errors

Lab products found in correlation

Texas red filter cube, by Leica (2 mentions) Dmi6000b microscope, by Leica (1 mentions) Application suite x software, by Leica (1 mentions) Mitotracker red cm h2xros, by Thermo Fisher Scientific (1 mentions) Dmi6000b, by Leica (1 mentions) Gm 8000, by Tokai Hit (1 mentions) Thunder fluorescence microscope, by Leica (1 mentions)

Spelling variants of this product

GFP filter (5 citations) ET GFP band pass filter cube (2 citations)

4 protocols using gfp filter cube

1

Microscopic Observation of Mitochondrial Dynamics

Check if the same lab product or an alternative is used in the 5 most similar protocols
Microscopic observation was performed as described previously [81 (link)]. TIM50-GFP were cultured in YPD, YPD with 5 mM CaCl2, 1 M NaCl/YPD, and 1 M NaCl/YPD with 5 mM CaCl2. Cells were harvested at the log phase (OD660 = 1.0), and 1 μl of the suspension cell was mixed with 2 μl of YPD on a glass slide. Images were obtained and processed using the DMI6000 B microscope and Leica Application Suite X software (Leica Microsystems, Germany). The GFP fluorescence was observed using the GFP filter cube (Leica cat. # 11513899). Mitochondria were stained with 100 nM of MitoTracker Red CM-H2Xros (M7513, Thermo Fisher Scientific, USA) for 30 min and then washed with 0.5 ml of YPD. The cells were then observed using RFP filter cubes (Leica cat. # 11513894).
Saeki N., Yamamoto C., Eguchi Y., Sekito T., Shigenobu S., Yoshimura M., Yashiroda Y., Boone C, & Moriya H. (2023). Overexpression profiling reveals cellular requirements in the context of genetic backgrounds and environments. PLOS Genetics, 19(4), e1010732.
+ Open protocol
+ Expand
2

Live Cell Imaging with Fluorescence Microscopy

Check if the same lab product or an alternative is used in the 5 most similar protocols
Live cell imaging was performed on an epifluorescence microscope (Leica DMI6000B) equipped with an on-stage CO2 incubation chamber (Tokai Hit GM-8000) and a motorized stage (Prior). An adaptive focus control was used to actively keep the image in focus during the period of imaging. A light-emitting diode (LED) light source (Lumencor Sola) was used as the fluorescence light source. For blue-light stimulation, pulsed blue light (200 ms pulse duration at 9.7 W/cm2) was used for GFP imaging and to initiate CRY2-CIB1 and CRY2-CRY2 interactions. For mCherry imaging, pulsed green light (200 ms pulse duration) was used. Fluorescence signal from GFP was detected using a commercial GFP filter cube (Leica; excitation filter 472/30, dichroic mirror 495, emission filter 520/35); fluorescence signal from mCherry was detected using a commercial Texas red filter cube (Leica; excitation filter 560/40, dichroic mirror 595, emission filter 645/75). The images for ERK or AKT translocation were acquired with an oil-immersion 100× objective, while the images for PC12 or DRG morphologies were acquired with 10× or 40× objectives. All experiments were imaged with a sensitive CMOS camera (PCO.EDGE 5.5) (PCO).
Duan L., Hope J.M., Guo S., Ong Q., François A., Kaplan L., Scherrer G, & Cui B. (2018). Optical Activation of TrkA Signaling. ACS synthetic biology, 7(7), 1685-1693.
+ Open protocol
+ Expand
3

Quantifying CRY2PHR-mCherry-Raf1 Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
We used the same excitation light power and excitation filter/dichroic mirror/emission filter for all imaging experiments in the neurite outgrowth assay. Fluorescence of GFP was detected using the GFP filter cube (Leica, excitation filter 472/30, dichroic mirror 495, emission filter 520/35); fluorescence from mCherry was detected using the commercial Texas Red filter cube (Leica, excitation filter 560/40, dichroic mirror 595, emission filter 645/75). The relative CRY2PHR-mCherry-Raf1 expression level was estimated by the mCherry fluorescence intensity. ImageJ was used to measure the average intensity of a transfected cell and that of the background. The CRY2PHR-mCherry-Raf1 expression level was thus proportional to the background-subtracted fluorescence intensity. We then sorted the intensity into three levels referred to as low, medium, and high corresponding to 0-30%, 31-75%, and 75-100% of the maximal background-subtracted fluorescence intensity (Fig. S7).
Zhang K., Duan L., Ong Q., Lin Z., Varman P.M., Sung K, & Cui B. (2014). Light-Mediated Kinetic Control Reveals the Temporal Effect of the Raf/MEK/ERK Pathway in PC12 Cell Neurite Outgrowth. PLoS ONE, 9(3), e92917.
+ Open protocol
+ Expand
4

Imaging GFP reporter expression in Arabidopsis root tips

Check if the same lab product or an alternative is used in the 5 most similar protocols
Seeds of the pEIL2:EIL2:GFP reporter line were sterilized as described above and grown for 7 days in the same conditions as described above. Seedlings were then incubated for 4 h in ½ strength MS liquid media supplemented with 10 µM ACC, 50 µM MG132, or both, alongside a control with an equal volume of DMSO added. Col-0 was also incubated with DMSO as a control for imaging. After incubation, seedling root tips were immediately imaged using a Leica Thunder fluorescence microscope with a GFP filter cube (excitation 450-490 nm; emission 500-550 nm).
Houben M., Vaughan‐Hirsch J., Mou W., & Poel B.V.d. (2022). Ethylene Insensitive 3-Like 2 (EIL2) is a Brassicaceae-specific transcriptional regulator involved in fine-tuning ethylene-dependent hypocotyl elongation, lateral root formation and flowering time in Arabidopsis thaliana.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!