Isopropyl alcohol

Manufactured by Thermo Fisher Scientific

Sourced in United States, United Kingdom, India, Canada, Belgium

Isopropyl alcohol is a colorless, flammable liquid commonly used as a cleaning and disinfecting agent in laboratory settings. It serves as a solvent and can be used to clean surfaces, equipment, and instruments.

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Close spelling variants of this product

Isopropyl alcohol/tetrahydrofuran/TFA Isopropyl alcohol (IPA), Isopropyl alcohol (HPLC grade)

106 protocols using isopropyl alcohol

3D Printed Biofunctional Microfluidic Devices

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Veroclear-RGD810 print material
and SUP706B support were purchased from Stratasys, Ltd. (Eden Prairie,
MN). Isopropyl alcohol was obtained from Thermo Fisher Scientific
(St. Louis, MO). Nichrome wire, 0.025 and 0.05 mm diameters, was purchased
from Alfa Aesar (Tewksbury, MA). Glycine, arginine, phenylalanine,
aspartic acid, fluorescein isothiocyanate isomer I (FITC), N-cyclohexyl-3-aminopropanesulfonic acid (CAPS), and boric
acid were purchased from Millipore Sigma-Aldrich (St. Louis, MO).
N-160 Solvent thinner and carbon ink were purchased from Ercon Inc.
(Wareham, MA). Poly(dimethylsiloxane) (PDMS) base and curing agent
(Sylgard 184) were purchased from Dow Corning (West Salzburg, MI).
Silicon wafer substrates were purchased from Empak Inc. (Arrowswest,
CO). SU-50 and SU-8 developers were purchased from Kayaku Advanced
Materials Inc. (Westborough, MA).

Selemani M.A., Castiaux A.D, & Martin R.S. (2022). PolyJet-Based 3D Printing against Micromolds to Produce Channel Structures for Microchip Electrophoresis. ACS Omega, 7(15), 13362-13370.

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Electrochemical Sensing of Bioactive Compounds

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Titanium tetrabutoxide
(Alfa Aesar), ethylenedioxythiophene
(EDOT), epinephrine hydrochloride, dopamine hydrochloride, ascorbic
acid, uric acid, potassium ferricyanide, 3-pentadecylphenol, and potassium
ferrocyanide were purchased from Sigma-Aldrich Chemicals Pvt. Ltd.,
India; ammonium persulfate (APS, Merck), potassium dihydrogen phosphate
(Merck), polydimethyl siloxane-PDMS (Dow Corning, Sylgard 184 silicone
elastomer kit), sodium hydroxide, tetrabutyl ammonium hexaflourophosphate
(Merck), acetonitrile (Spectrochem Pvt. Ltd.), isopropyl alcohol (Fischer
Scientific), distilled water, and ethanol. All of the chemicals were
used as received. PDPSA was prepared as reported earlier from our
group.^{50 (link)}

Aashish A., Sadanandhan N.K., Ganesan K.P., Saraswathy Hareesh U.N., Muthusamy S, & Devaki S.J. (2018). Flexible Electrochemical Transducer Platform for Neurotransmitters. ACS Omega, 3(3), 3489-3500.

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Isolation and Preservation of Lymphatic Vessels

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Tissue specimens were transported directly from surgical theatre to the laboratory in ice-cold sterile Phosphate Buffered Saline (VWR, UK). LVs were identified and isolated along with a small amount of surrounding tissue under a dissection microscope within a sterile laminar flow cabinet (Fig 1A–1C). Each vessel was divided into two sections. One half of each vessel was further cleaned of surrounding fatty tissue, placed in RNA stabilising solution (RNAlater, ThermoFisher, UK), frozen on dry ice and stored at -20°C. The other half was fixed in 4% paraformaldehyde (VWR, UK) for 45 minutes, followed by submersion in 15% sucrose for 3 hours, then 30% sucrose for 6–12 hours. This was followed by embedding in OCT (Scigen, UK) using dry ice and isopropyl alcohol (ThermoFisher, UK) and stored at -80°C.

Johnson S.C., Chakraborty S., Drosou A., Cunnea P., Tzovaras D., Nixon K., Zawieja D.C., Muthuchamy M., Fotopoulou C, & Moore JE J.r. (2020). Inflammatory state of lymphatic vessels and miRNA profiles associated with relapse in ovarian cancer patients. PLoS ONE, 15(7), e0230092.

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SiC Synthesis and Purification from PDMS

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Samples prepared with 100% power were generally used for crystal structure and property analysis and for direct integration with cells via dispersion of the crystals in growth media. However, before these processes, the crystals were purified. The SiC along with the graphite layer was cut out from the PDMS matrix using a razor blade. The sample was then washed in toluene (ThermoFisher Scientific) for 5 min to remove any organic components present in the material. Following this, the sample was immersed in isopropyl alcohol (ThermoFisher Scientific) for 5 min and then in deionized (DI; 18.2 megohm) water for 5 min. After this sequential washing, the samples were dipped in 1:1 HF:HNO₃ (Sigma-Aldrich) for 5 min to remove any silica and surface organics. Last, the samples were washed in DI water, dried using a nitrogen blow dry process, and oxygen plasma cleaned at 200 W for 3 min (PE-100 Benchtop Plasma System). After the final step, the SiC synthesized from pure PDMS appeared yellow (fig. S1g).

Nair V., Yi J., Isheim D., Rotenberg M., Meng L., Shi F., Chen X., Gao X., Prominski A., Jiang Y., Yue J., Gallagher C.T., Seidman D.N, & Tian B. (2020). Laser writing of nitrogen-doped silicon carbide for biological modulation. Science Advances, 6(34), eaaz2743.

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Quantitative Skin Lipid Analysis

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LC–MS-grade acetonitrile (ACN, catalog no. A955-4), methanol (MT, catalog no. A456-4), ammonium formate (catalog no. A115-50), isopropyl alcohol (IPA, catalog no. A461-4), N-propanol (NPA, catalog no. A414-4), acetonitrile (catalog no. A955-4), and formic acid (catalog no. LS118-4) were obtained from Thermo Fisher (Waltham, MA, USA). skin surface lipids (SSL)-adsorbent tapes Corneofix^® (Cologne, Germany) and a Mass Spectrometer (Waters UPLC-VION QTOF MS, Milford, MA, USA) were used for this study.

Chen T., Wang J, & Mao Z. (2023). Lipid Differences and Related Metabolism Present on the Hand Skin Surface of Different-Aged Asiatic Females—An Untargeted Metabolomics Study. Metabolites, 13(4), 553.

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Cryopreservation and Thawing of IPTD-MSCs

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Isolated IPTD-MSCs (at passage 3) were cultured to ~ 80% confluence, dissociated into single cells with TrypLE, collected, and counted. Aliquots of 1 × 10⁶ cells were divided into cryopreservation tubes, suspended in 10% DMSO in CMRL-1066 medium, and stored at − 80 °C in a Mr. Frosty Freezing apparatus containing 100% isopropyl alcohol (ThermoFisher). Using this method, IPTD-MSCs were stored for 9 months. The cells were then thawed rapidly in a 37 °C water bath, washed with DPBS, and cultured in T-75 tissue culture flasks using CMRL media with 5% hPL. After 48 h, the cells were noted to be ~ 80% confluent and were subjected to subsequent analyses. Viability was assessed with trypan blue.

Khiatah B., Qi M., Du W., T-Chen K., van Megen K.M., Perez R.G., Isenberg J.S., Kandeel F., Roep B.O., Ku H.T, & Al-Abdullah I.H. (2019). Intra-pancreatic tissue-derived mesenchymal stromal cells: a promising therapeutic potential with anti-inflammatory and pro-angiogenic profiles. Stem Cell Research & Therapy, 10, 322.

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Acetylcholinesterase Inhibition Assay

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Bovine serum albumin (BSA) was purchased from VWR International (Radnor, PA), while acetylthiocholine iodide (ATCI), sodium citrate, sodium chloride, potassium chloride, sodium carbonate, monosodium phosphate, and disodium phosphate were purchased from Sigma‐Aldrich (St. Louis, MO). 2‐[4‐(2‐hydroxyethyl)piperazin‐1‐yl]ethanesulfonic acid (HEPES) was purchased from Hampton Research. Hexanes, 5,5′‐dithiobis(2‐nitrobenzoic acid) (DTNB), and isopropyl alcohol were purchased from Thermo Fisher Scientific (Waltham, MA). Laboratory deionized water of >17 MΩ was used for all assays.

Bester S.M., Adipietro K.A., Funk V.L., Myslinski J.M., Keul N.D., Cheung J., Wilder P.T., Wood Z.A., Weber D.J., Height J.J, & Pegan S.D. (2019). The structural and biochemical impacts of monomerizing human acetylcholinesterase. Protein Science : A Publication of the Protein Society, 28(6), 1106-1114.

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Cell Cryopreservation: A Standardized Protocol

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For cell cryopreservation, an appropriate number of 2 mL cryovials was labeled (indicating the cell line, passage, date, cell numbers, and cryopreservation media). After automatic counting and determination of cell viability, the desired number of cells suspended in 1 mL of culture medium was transferred to each vial. After adding cryopreserving agent, dimethyl sulfoxide (DMSO—10% (v/v)), and more medium to fill the vials, they were capped and transferred into a freezing container with isopropyl alcohol (ThermoScientific^®, Waltham, MA, USA) for slow freezing (−1 °C/minute) at −80 °C for 3 days. For longtime storage, vials were transferred to liquid nitrogen storage containers (−196 °C) (Wharton^®, Philadelphia, PA, USA).

Ribeiro E., Araújo D., Pereira M., Lopes B., Sousa P., Sousa A.C., Coelho A., Rêma A., Alvites R., Faria F., Oliveira C., Porto B., Maurício A.C., Amorim I, & Vale N. (2023). Repurposing Benztropine, Natamycin, and Nitazoxanide Using Drug Combination and Characterization of Gastric Cancer Cell Lines. Biomedicines, 11(3), 799.

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Meropenem Quantification in Biological Matrices

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Meropenem trihydrate (lot no.: 130506–202004; purity 99.0%) was purchased from the National Institutes for Food and Drug Control (Beijing, China). Meropenem-d₆ (lot no.: K2202661; purity 97.5%) was obtained from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). LC–MS grade methanol, acetonitrile and isopropyl alcohol were obtained from Thermo Fisher Scientific (Lenexa, KS, USA). HPLC grade formic acid, analytical grade ethylene glycol and sodium hydroxide (NaOH) were purchased from Kelong Chemical (Chengdu, China). HPLC grade 3-morpholinopropanesulfonic acid (MOPS) was provided by Macklin Biochemical Technology Co., Ltd. (Shanghai, China). Purified water was produced by a Millipore Milli-Q IQ 7000 system (Boston, MA, USA). Blank serum was obtained from the Chengdu Blood Center (Chengdu, China), and artificial cerebrospinal fluid was obtained from Coolaber Science & Technology Co., Ltd. (Beijing, China). Six lots of blank cerebrospinal fluid (CSF) were obtained from the Neurology Department of West China Hospital of Sichuan University (Chengdu, China).

Jin Y., Ma H., Fu L., Qi X., Zhang M., Di X., Zheng L., He C, & Wang Z. (2024). Quantification of meropenem in serum and cerebrospinal fluid in children with bacterial meningitis with augmented renal clearance by UPLC–MS/MS. Heliyon, 10(4), e26132.

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Purification and Characterization of INI-4001 and H7 Antigen

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INI-4001 was synthesized and processed to over 99% purity as described previously [18 (link),29 (link)]. Hemagglutinin antigen trimer (H7) was obtained from Dr. Florian Krammer at the Icahn School of Medicine at Mount Sinai [30 (link),31 (link)]. All chemicals used were of analytical reagent grade and all solvents were of HPLC grade. Trifluoroacetic acid (TFA) and ammonium hydroxide were obtained from J.T. Baker (Phillipsburg, NJ, USA). Sterile water for irrigation (WFI) was obtained from Baxter Healthcare Corp (Deerfield, IL, USA). Methanol, anhydrous ethanol, isopropyl alcohol, Triton X-100, glycerol, ammonium formate, acetone, tetrahydrofuran, methyl t-butyl ether, and sodium hydroxide were obtained from Thermo Fisher Scientific (Waltham, MA, USA). (3-Aminopropyl) triethoxysilane (APTES) was obtained from Sigma-Aldrich (St. Louis, MO, USA). 50 and 200 nm solid SNP were purchased as ethanol suspensions (10 mg/mL) from NanoComposix (San Diego, CA, USA). RPMI medium 1640 (Cat. No. 11875-093, Gibco, Thermo Fisher Scientific, Waltham, MA, USA) was supplemented with 10% fetal bovine serum (Cat. No. 35-011-CV, Corning Inc., Corning, NY, USA), 100 U/mL penicillin, 100 μg/mL streptomycin, and 292 μg/mL glutamine (Cat. No. 10378-016, Thermo Fisher) to prepare the complete RPMI media (RP-10).

Abdelwahab W.M., Auclair S., Borgogna T., Siram K., Riffey A., Bazin H.G., Cottam H.B., Hayashi T., Evans J.T, & Burkhart D.J. (2024). Co-Delivery of a Novel Lipidated TLR7/8 Agonist and Hemagglutinin-Based Influenza Antigen Using Silica Nanoparticles Promotes Enhanced Immune Responses. Pharmaceutics, 16(1), 107.

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