Du401a bv

Manufactured by WITec

Sourced in United Kingdom

The DU401A-BV is a high-performance digital multimeter designed for laboratory and industrial applications. It features a large, easy-to-read display, and provides accurate measurements of voltage, current, resistance, and other electrical parameters. The DU401A-BV is built to provide reliable and consistent performance in a variety of settings.

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Lab products found in correlation

Uhts 300, by WITec (4 mentions) Alpha300ra, by WITec (3 mentions) Igor pro 7, by Wavemetrics (1 mentions) Jem 1011, by JEOL (1 mentions) Alpha 300, by WITec (1 mentions) Uv 2600 spectrophotometer, by Shimadzu (1 mentions) Jem 2200f, by JEOL (1 mentions) Objective lens, by Nikon (1 mentions) Control four acquisition software, by WITec (1 mentions) Project five plus, by WITec (1 mentions) Opus 7, by Bruker (1 mentions)

4 protocols using du401a bv

Confocal Raman Mapping of Calcifications

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A confocal Raman microscope (alpha300RA, WITec, Ulm, Germany) with a frequency-doubled Nd:YAG laser (532 nm) excitation source was used (maximum power: 75 mW). The charge-coupled device detector (DU401A-BV, Andor, UK) was placed behind the spectrometer (UHTS 300, WITec, Ulm, Germany) with a grating of 600 g/mm (Blaze wavelength = 500 nm). Samples were placed on a multi-axis piezo scanner and a motorized large-area stage for sample positioning and imaged using a water immersion 60× objective [Nikon, numerical aperture (NA) = 1.0]. The lateral resolution was 0.61 λ/NA = ~325 nm. The typical mapping scanning step size used was 1 μm (though ranged from 0.5 to 2 μm) with a typical integration time of 0.23 s per step (though going as high as 4 s per step in rare cases).
Mapping data from 117 regions (from 96 individual calcifications and 5 particle-containing regions) containing Raman-detected calcifications with pathological continuity were analyzed. All Raman data were analyzed using WITec Project Plus version 4.1 and, where indicated, Igor Pro 7 (WaveMetrics Inc., Lake Oswego, OR, USA). Raman data processing is elaborated in Supplementary Text.

Kunitake J.A., Sudilovsky D., Johnson L.M., Loh H.C., Choi S., Morris P.G., Jochelson M.S., Iyengar N.M., Morrow M., Masic A., Fischbach C, & Estroff L.A. (2023). Biomineralogical signatures of breast microcalcifications. Science Advances, 9(8), eade3152.

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Confocal Raman Mapping of PVA Hydrogels

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Each sample was hydrated for more than 2 hours and then pressed between a glass slide and a coverslip to ensure a flat surface. The coverslip was then sealed at the edges with nail polish to prevent the hydrogel from drying. A confocal Raman microscope (alpha300 RA, WITec, Germany) with 20× objective (Zeiss, Germany) was used. An Nd:YAG laser (532 nm) was used as the excitation source with the maximum power of 75 mW. Data were collected using a charge-coupled device detector (DU401A-BV, Andor, UK) behind a grating spectrometer (600 g/mm; UHTS 300, WITec, Germany). A 20-μm-resolution Raman map of 4 × 3–mm scan area was acquired with an accumulation time of 1 s per point. Each point was prebleached for 400 ms to decrease the effect of fluorescence. Cosmic ray removal and background subtraction were performed to clean the spectra. The intensity of O–H bond within the PVA and water was calculated by integrating the spectra in the range of 2800 to 3000 cm⁻¹ and 3075 to 3625 cm⁻¹, respectively. The ratio of PVA and water was then calculated and plotted as a heatmap shown in Fig. 5A.

Lin S., Liu X., Liu J., Yuk H., Loh H.C., Parada G.A., Settens C., Song J., Masic A., McKinley G.H, & Zhao X. (2019). Anti-fatigue-fracture hydrogels. Science Advances, 5(1), eaau8528.

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Characterization of Gold Nanoparticles

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A Shimadzu UV-2600 spectrophotometer was used in the wavelength range between 200 and 1400 nm to obtain UV-vis-NIR absorption spectra. The morphology of the different gold nanoparticles was identified by using a transmission electron microscope JEM-1011 (JEOL) at an acceleration voltage of 80 kV as well as the JEM-2200 FS (JEOL) at 200 kV for high resolution (HRTEM), fast Fourier transformation (FFT) and element mapping (EDX measurements). For obtaining SEM micrographs a ZEISS Supra 55PV scanning electron microscope at an acceleration voltage of 2 kV was used. The zeta potential was determined with a Malvern Nano Zetasizer 3600. The SERS performance was assessed using a confocal Raman microscope Alpha 300 (WITec) coupled with laser excitation at wavelength of 785 nm. The 2 and 10 mW laser beam was focused on the sample through a Nikon 20× objective lens. The Raman spectra were acquired with a thermoelectrically cooled Andor CCD detector DU401A-BV placed behind the spectrometer UHTS 300 from WITec with a spectral resolution of 3 cm⁻¹, and were recorded with an integration time of 2 seconds, which is also the exposure time of the laser beam. The Raman band of the silicon wafer at 520 cm⁻¹ was used to calibrate the spectrometer.

Liebig F., Sarhan R.M., Bargheer M., Schmitt C.N., Poghosyan A.H., Shahinyan A.A, & Koetz J. (2020). Spiked gold nanotriangles: formation, characterization and applications in surface-enhanced Raman spectroscopy and plasmon-enhanced catalysis. RSC Advances, 10(14), 8152-8160.

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Raman Spectroscopy of Nanoparticle Powders

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For Raman measurements, the nanoparticle powders were placed on a standard glass slide. A Confocal Raman microscope (CRM) (alpha 300 RA, WITec GmbH, Ulm, Germany) equipped with a piezo motorized scan stage (x-y) was used. The excitation light source was a linearly polarized (0°) coherent compass sapphire green laser λ 532 nm (WITec, Ulm, Germany) focused through a 20× objective (Carl Zeiss, Jena, Germany). The Raman scattering signal was collected by the same objective, delivered by an optic multifiber (∅ = 50 µm) to the spectrometer (600 g·mm⁻¹ grating, UHTS 300 WITec, Ulm, Germany) and recorded by an attached CCD camera (Andor DU401ABV, Belfast, North Ireland). The Control Four acquisition software (WITec, Ulm, Germany) was used for control of the measurement. The laser power for all measurements was set to 40 mW and the integration time was 60 s. Project FIVE Plus (WITec, Ulm, Germany) was used for spectral processing and data analysis. The extracted spectra were analyzed with Opus 7.5 software (Bruker, Bremen, Germany).

Willinger M., Felhofer M., Reimhult E, & Zirbs R. (2021). Method for High-Yield Hydrothermal Growth of Silica Shells on Nanoparticles. Materials, 14(21), 6646.

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