Db 5ms ui

Manufactured by Agilent Technologies

Sourced in United States

The DB-5MS UI is a capillary column designed for gas chromatography (GC) applications. It features a 5% phenyl-methylpolysiloxane stationary phase and is suitable for a wide range of analytes, including both polar and non-polar compounds. The column provides high thermal stability and inertness, making it a reliable choice for various analytical tasks.

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Lab products found in correlation

Gcms qp2020, by Shimadzu (3 mentions) Agilent 7890b gc, by Agilent Technologies (1 mentions) Sq456 gc ms, by Agilent Technologies (1 mentions) Gc vial, by Agilent Technologies (1 mentions) Chloroform, by Merck Group (1 mentions) Multi purpose sampler mps, by Gerstel (1 mentions) Masshunter qualitative analysis software, by Agilent Technologies (1 mentions) 5977b mass spectrometer, by Agilent Technologies (1 mentions) Agilent 7890b gc system, by Agilent Technologies (1 mentions) 450 gc 240 ms, by Agilent Technologies (1 mentions) Clarus 600 gas chromatography, by PerkinElmer (1 mentions) Turbo matrix 650 automatic thermal desorber, by PerkinElmer (1 mentions)

Close spelling variants of this product

DB-5MS (222 citations) DB-5MS UI column (16 citations) DB-5MS UI capillary column (6 citations) GC DB-5MS UI fused silica column (2 citations)

12 protocols using db 5ms ui

GC-MS VOC Analysis Protocol

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The principal analytical platform used
for VOC analysis was a TOF-based Markes TD100XR thermal desorber coupled
with an Agilent 7890B GC and a Markes BenchTOF select MS (Markes International,
Llantrisant, UK). A Markes TD100 thermal desorber (Markes International,
Llantrisant, UK) coupled with an Agilent 7890B GC and a quadrupole
5977A MS system served as a reference assay. Chromatographic separation
was performed for the polar TOF assay using a Mega WAX-HT, (20 m ×
0.18 mm × 0.18 μm, MEGA S.r.l., Legnano, Italy), for the
nonpolar TOF assay using a DB5-MS UI (30 m × 0.25 mm × 1.00
μm, Agilent Technologies, Santa Clara, USA) and for the reference
quadrupole MS assay using a Zebron ZB-624UI, 60 m × 0.25 mm ×
1.40 μm (Phenomenex, Torrance, USA) capillary column.

Wen Q., Myridakis A., Boshier P.R., Zuffa S., Belluomo I., Parker A.G., Chin S.T., Hakim S., Markar S.R, & Hanna G.B. (2023). A Complete Pipeline for Untargeted Urinary Volatolomic Profiling with Sorptive Extraction and Dual Polar and Nonpolar Column Methodologies Coupled with Gas Chromatography Time-of-Flight Mass Spectrometry. Analytical Chemistry, 95(2), 758-765.

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GC-MS Analysis of HWE-Extracted Fractions

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Pyrolab2000
(Pyrolab, Sweden) analytical-scale equipment directly connected to
Bruker Scion SQ456-GC/MS equipped with an Agilent DB-5MS UI (5%-phenyl)-methylpolysiloxane,
30 m × 0.250 mm × 0.25 μm l m) capillary column was
used for analysis. HWE-extracted fractions (A) were analyzed, and
carbohydrate versus aromatic fragment ratios were estimated according
to Kuuskeri et al.^{49 (link)} Details of the analysis
method are given in S1.7.

Hyväkkö U., Maltari R., Kakko T., Kontro J., Mikkilä J., Kilpeläinen P., Enqvist E., Tikka P., Hildén K., Nousiainen P, & Sipilä J. (2019). On the Effect of Hot-Water Pretreatment in Sulfur-Free Pulping of Aspen and Wheat Straw. ACS Omega, 5(1), 265-273.

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Pyrolysis GC-MS Analysis Protocol

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Measurements
were performed using a Pyrolab2000 pyrolyzer connected to a Bruker
Scion SQ 456 GC–MS. The pyrolysis chamber temperature was kept
at 150 °C and the samples were pyrolyzed by heating the platinum
filament in 8 ms to 200 °C and keeping the temperature elevated
for 2 s before rapid cooling of the filament. The second heating to
580 °C was also 2 s long. Helium was used as a carrier gas at
a flow rate of 1 mL min^–1. Injector temperature
was 250 °C and a 1:20 split ratio was used. Pyrolysis products
were separated in an Agilent DB-5MS UI [(5%-phenyl)-methylpolysiloxane,
30 m × 0.250 mm × 0.25 μm film] capillary column.
Column oven temperature was kept at 50 °C for 2 min after which
the temperature was increased at a rate of 10 °C min^–1 to 280 °C and kept at 280 °C for 5 min resulting in a
30 min overall run time. Ion source temperature was 250 °C with
an electron ionization of 70 eV. The MS scan range was 40–400 m/z. Compounds were identified by comparing
them to the National Institute of Standards and Technology (NIST)
database.

Lahtinen M.H., Mikkilä J., Mikkonen K.S, & Kilpeläinen I. (2021). Kraft Process—Formation of Secoisolariciresinol Structures and Incorporation of Fatty Acids in Kraft Lignin. Journal of Agricultural and Food Chemistry, 69(21), 5955-5965.

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GC-MS Analysis of Organic Compound Fractions

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The sample was separated on a fused-silica capillary column (DB-5ms UI, 30 m × 0.25 mm i.d., film thickness 0.25 μm, Agilent, Santa Clara, CA, USA) installed on GCMS-QP2020 (Shimadzu, Kyoto, Japan). The oven temperature was programmed as isothermic at 60 °C for 2 min, 100 °C at 4 °C/min, 290 °C at 10 °C/min, and finally isothermic for 10 min. The split injection mode (1:10) was used. The carrier gas was helium at a constant flow rate of 1 mL/min. The injection port, ion source, and interface temperatures were 280, 280, and 150 °C, respectively. The energy of ionization was 70 eV. The mass spectra were obtained in full scan mode (40-700 AMU). The hexane and ethyl acetate fractions (1 μL, 1 mg/mL) were injected into the GC/MS via an auto-injector. The unknown compounds were identified by matching known compounds in the National Institute of Standards and Technology (NIST) library.

Sharma H., Kim D.Y., Shim K.H., Sharma N, & An S.S. (2023). Multi-Targeting Neuroprotective Effects of Syzygium aromaticum Bud Extracts and Their Key Phytocompounds against Neurodegenerative Diseases. International Journal of Molecular Sciences, 24(9), 8148.

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Pyrolysis-GC-MS Analysis of Biomass

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Measurements were
performed using Pyrolab2000
pyrolyzer connected to Bruker Scion SQ 456 GC–MS. The pyrolysis
chamber temperature was kept at 150 °C, and samples were pyrolyzed
isothermally by heating the platinum filament in 8 ms to 580 °C
and keeping the temperature elevated for 2 s before rapid cooling
of the filament. Helium was used as a carrier gas at the flow rate
of 1 mL min^–1. The injector temperature was 250
°C, and 1:2 split ratio was used. Pyrolysis products were separated
in an Agilent DB-5MS UI ((5%-phenyl)-methylpolysiloxane, 30 m ×
0.250 mm × 0.25 μm film) capillary column. The column oven
temperature was kept at 50 °C for 2 min, after which the temperature
was increased at 10 °C min^–1 rate to 280 °C,
resulting in a 25 min overall run time. The ion source temperature
was 250 °C with electron ionization of 70 eV. The MS scan range
was 40–400 m/z. Twenty-five
peaks were detected from the total ion count chromatograms between
3.3 and 14.3 min retention times, and 22 of the compounds could be
identified by comparing to standard sample runs, to the National Institute
of Standards and Technology (NIST) library, and to the literature.^{48 (link),51 (link)−55 (link)}

Mikkilä J., Trogen M., Koivu K.A., Kontro J., Kuuskeri J., Maltari R., Dekere Z., Kemell M., Mäkelä M.R., Nousiainen P.A., Hummel M., Sipilä J, & Hildén K. (2020). Fungal Treatment Modifies Kraft Lignin for Lignin- and Cellulose-Based Carbon Fiber Precursors. ACS Omega, 5(11), 6130-6140.

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GC-MS Analysis of Organic Compounds

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The sample was analyzed on a fused-silica capillary column (DB-5ms UI, 30 m × 0.25 mm i.d., film thickness 0.25 μm, Agilent, Santa Clara, CA, USA) installed on a GCMS-QP2020 (Shimadzu, Kyoto, Japan). The oven temperature was programmed at 60 °C for 2 min, 100 °C at 4 °C/min, 290 °C at 10 °C/min, and finally to isothermic for 10 min. The split injection mode (1:10) was used and hexane and ethyl acetate fractions (1 μL, 1 mg/mL) were injected into the GC/MS via an auto-injector. The carrier gas was helium at a constant flow mode rate of 1 mL/min. The injection port, ion source, and interface temperatures were: 280, 280, and 150 °C, respectively. The energy of ionization was 70 eV. The mass spectra were obtained in full scan mode (40–700 AMU).

Sharma H., Sharma N, & An S.S. (2023). Black Pepper (Piper nigrum) Alleviates Oxidative Stress, Exerts Potential Anti-Glycation and Anti-AChE Activity: A Multitargeting Neuroprotective Agent against Neurodegenerative Diseases. Antioxidants, 12(5), 1089.

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GC-MS Analysis of Compound Identification

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Identification of
compounds was performed
by GC-MS using a 7890B gas chromatogram interfaced with a 5977B mass
spectrometer (Agilent, Santa Clara, CA) coupled to a GERSTEL multipurpose
sampler (MPS) (GERSTEL, Linthicum, MD). The column used was a DB-5MS
UI (30 m, 0.25 mm I.D., 0.25 μm) (Agilent, Santa Clara, CA).
The oven had an initial temperature of 150 °C that was increased
at a rate of 4 °C/min until reaching 300 °C. The inlet temperature
was 300 °C, the helium flow rate was 1 mL/min, and 1 μL
of the sample was injected. The mass spectrometer parameters were
as follows: the ionization mode was electron ionization (EI), and
the ion source temperature was 230 °C. For the methanol extract
samples, 1 mL of solution was transferred to a GC vial (Agilent, Santa
Clara, CA). A split of 1:5 was used, the m/z range was 50–500, and the solvent delay was 8 min.
For the alkaloid extract samples, the residue was resuspended in chloroform
(Sigma-Aldrich, St. Louis, MO), filtered, and transferred to a GC
vial. A split of 1:10 was used, the m/z range was 50–600, and the solvent delay was 3.5 min. Data
processing, which included automatic and manual peak integration,
was performed using MassHunter Qualitative Analysis Software (Agilent,
Santa Clara, CA).

Coon A.M, & Musah R.A. (2023). Investigation of Small-Molecule Constituents in Voacanga africana Seeds and Mapping of Their Spatial Distributions Using Laser Ablation Direct Analysis in Real-Time Imaging–Mass Spectrometry (LADI-MS). ACS Omega, 8(30), 27190-27205.

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GC-MS Analysis of Plant Extracts

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The hexane and ethyl acetate fractions (1 μL, 1 mg/mL) were analyzed on a fused-silica capillary column (DB-5 ms UI, 30 m × 0.25 mm i.d., film thickness 0.25 μm, Agilent, Santa Clara, CA, USA) connected to GCMS-QP2020 (Shimadzu, Kyoto, Japan). The oven temperature was set (60 °C/2 min, 100 °C at 4 °C/min, 290 °C at 10 °C/min, isothermic for 10 min). The carrier gas, helium, was at a constant flow rate of 1 mL/min. The temperature was set for the injection port (280 °C), ion source (280 °C), and interface (150 °C). The ionization energy was set at 70 eV. The full scan mode (40–700 AMU) was used to obtain the mass spectra. The phytocompounds in the fractions were identified by comparing them with known compounds in the National Institute of Standards and Technology (NIST) library.

Sharma H., Yang H., Sharma N, & An S.S. (2023). Trachyspermum ammi Bioactives Promote Neuroprotection by Inhibiting Acetylcholinesterase, Aβ-Oligomerization/Fibrilization, and Mitigating Oxidative Stress In Vitro. Antioxidants, 13(1), 9.

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Chlorothalonil Analysis by GC-MS/MS

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Gas chromatography was performed for chlorothalonil analysis using an Agilent 7890 B GC system coupled to a 7010 B triple quadrupole (Agilent Technologies, Santa Clara, CA, USA). Agilent MassHunter QQQ Acquisition and Quantitative Analysis software version 10.0. was used for data acquisition and quantification. The column used for analysis was a DB-5MS UI (30 m × 0.25 mm, 0.25 µm, Agilent, Santa Clara, CA, USA). High-purity helium was used as a carrier gas at a flow rate of 1.5 mL/min. The injection mode was splitless, and the volume was 1 µL. The interface temperature was 280 °C. The conditions of the oven temperature are presented in Table S1.

Yun D.Y., Bae J.Y., Park C.W., Jang G.H, & Choe W.J. (2023). Determination of Modified QuEChERS Method for Chlorothalonil Analysis in Agricultural Products Using Gas Chromatography–Mass Spectrometry (GC-MS/MS). Foods, 12(20), 3793.

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Metabolite Profiling by GC-MS

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Ten mg of freeze-dried MRE were resuspended in 120 μl methanol containing 0.2 mg/ml ribitol as internal standard. The solution was dried under nitrogen and derivatized by the addition of 60 μl pyridine containing 20 mg/ml methoxyamine. After brief vortexing, the resulting solution was incubated at 70°C for 120 min. After cooling and a brief centrifugation at 1000 ×g, 100 μl of N,O-bis(trimethylsilyl)trifluoroacetamide with 1% (v/v) of the silylation agent trimethylchlorosilane were added and incubated at 70°C for 30 min. Once cooled down, 1 μl samples were injected at 230°C into a 450-GC 240 MS (Varian) Gas chromatography-mass spectrometer using the split mode (100:1). The He carrier gas flow was at 1 ml/min and the capillary chromatography column DB-5MS UI 30 mm × 0.25 mm × 0.25 μm (Agilent Technologies) was used. Column oven ramp was set as follows: 70°C (5 min), to 245°C at 5°C/min, to 310°C at 20°C/min, 310°C (1 min). Electron Impact Ionization was at 70 eV and detection was carried out in Full Scan mode with acquisition between 50 and 600 m/z. Compound identification was achieved using the NIST-08 Standard reference database¹ and data comparison with compound standards.

López-Farfán D., Reyes-Darias J.A., Matilla M.A, & Krell T. (2019). Concentration Dependent Effect of Plant Root Exudates on the Chemosensory Systems of Pseudomonas putida KT2440. Frontiers in Microbiology, 10, 78.

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