Rimonabant

Manufactured by Bio-Techne

Sourced in United Kingdom

Rimonabant is a laboratory reagent used in research applications. It functions as an antagonist of the cannabinoid receptor CB1. This information is provided without further interpretation or extrapolation on its intended use.

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Lab products found in correlation

Close spelling variants of this product

Rimonabant hydrochloride (4 citations) Rimonabant (RIM; (2 citations) Rimonabant/SR141716A (2 citations)

11 protocols using rimonabant

Pharmacological Modulation of Neuronal Signaling

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Reagents were purchased from Sigma except for GABAzine (SR95531, Tocris#1262), CGP55845 (Tocris#1248), D-AP5 (Tocris#0106), DNQX (Tocris#2312), Noradrenaline (Abcam#ab120717), HEAT (Tocris#0535), Phenylephrine (Tocris#2838), Isoproterenol (Tocris#1747), Win (Tocris#1038), AM251 (Tocris#1117), Rimonabant (Tocris#0923), MPEP (Tocris#1212), CPCCOEt (Tocris#1028), CNO (Hello Bio#HB6149), Cy5-streptavidin (ThermoFisher#434316). For i.p. injection, Rimonabant (Tocris #0923) was first prepared 30 mg/mL stock in DMSO, then diluted in saline with 1% DMSO, 1% Cremophor, 1% Ethanol to make 0.3 mg/mL for injection, finally injected 0.01 mL/g to reach 3 mg/kg injection. Phenylephrine (Tocris #2838) was prepared at 10 mM stock solution in water, then diluted in saline for injection. CNO (HelloBio #HB6149) was prepared 0.1 mg/mL in saline and injected 0.01 mL/1 g to reach 1 mg/kg injection. For slice recordings, all stocks were diluted to final concentrations in ACSF to achieve a final solvent concentration of less than 0.1% v/v

Yang B., Sanches-Padilla J., Kondapalli J., Morison S.L., Delpire E., Awatramani R, & Surmeier D.J. (2021). Locus coeruleus anchors a trisynaptic circuit controlling fear-induced suppression of feeding. Neuron, 109(5), 823-838.e6.

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Rimonabant Dosage and Administration

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The CB1-R antagonist/inverse agonist rimonabant (SR141716) was purchased from Tocris (Tocris Bioscience, Bio-Techne, Minneapolis, Minnesota). Drugs were dissolved in dimethyl sulfoxide:Tocrisolve:saline solution at the ratio of 1:1:18 and vortexed to obtain a stable emulsion for bolus intravenous or intraperitoneal injections (26) (link). rimonabant was administered at the dose of 5 mg/kg.

Paloczi J., Matyas C., Cinar R., Varga Z.V., Hasko G., Schindler T.H., Kunos G, & Pacher P. (2019). Alcohol Binge-Induced Cardiovascular Dysfunction Involves Endocannabinoid–CB1-R Signaling. JACC: Basic to Translational Science, 4(5), 625-637.

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Chemical Sympathectomy and CB1 Receptor Modulation

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Rimonabant (Tocris) was dissolved in 4% DMSO and 1% Tween-80 in saline and administered i.p at 5 mg/kg (10 mL/kg). Naltrexone (Tocris) was dissolved in saline and administered i.p at 10 mg/kg (10 mL/kg). Naloxone (Sigma-Aldrich) was dissolved in saline and administered i.p at 2 mg/kg (10 mL/kg). CGS15943 was dissolved in 5% DMSO, 0.3% Tween-80 in saline and administered i.p at 10 mg/kg (1 mL/kg). For induction of chemical sympathectomy, 6-OHDA free base (Tocris) was dissolved in 0.1% ascorbic acid in saline and administered i.p at 100 mg/kg (10 mL/kg) one week prior to ET injection. BCI (EMD Millipore) was dissolved in 1% DMSO in saline and administered intrathecally (1.6 μg in 5 μL).

Yang N.J., Isensee J., Neel D., Quadros A.U., Zhang H.X., Lauzadis J., Liu S.M., Shiers S., Belu A., Palan S., Marlin S., Maignel J., Kennedy-Curran A., Tong V.S., Moayeri M., Röderer P., Nitzsche A., Lu M., Pentelute B.L., Brüstle O., Tripathi V., Foster K.A., Price T.J., Collier R.J., Leppla S.H., Puopolo M., Bean B.P., Cunha T.M., Hucho T, & Chiu I.M. (2021). Anthrax toxins regulate pain signaling and can deliver molecular cargoes into Antxr2+ DRG sensory neurons. Nature neuroscience, 25(2), 168-179.

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Solubilization of Bioactive Compounds

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Capsaicin, capsazepine, olvanil and rimonabant were purchased from Tocris Bioscience (UK). Drugs were initially dissolved in ethanol (100 %) to form a stock solution (Capsaicin 1 mM, capsazepine 10 mM, olvanil 10 mM and rimonabant 10 mM), then diluted in phosphate-buffered saline (PBS). Final ethanol concentrations in the solutions used in the present study did not exceed 0.1 %.

Alsalem M., Millns P., Altarifi A., El-Salem K., Chapman V, & Kendall D.A. (2016). Anti-nociceptive and desensitizing effects of olvanil on capsaicin-induced thermal hyperalgesia in the rat. BMC Pharmacology & Toxicology, 17, 31.

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Cellular Signaling Compounds Protocol

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Ionomycin calcium salt from Streptomyces conglobatus was purchased from SigmaAldrich (St Louis, MO), and Arachidonyl-2′-chloroethylamide (ACEA) and rimonabant from Tocris Bioscience (Bristol, UK).

Angelats E., Requesens M., Aguinaga D., Kreutz M.R., Franco R, & Navarro G. (2018). Neuronal Calcium and cAMP Cross-Talk Mediated by Cannabinoid CB1 Receptor and EF-Hand Calcium Sensor Interactions. Frontiers in Cell and Developmental Biology, 6, 67.

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Cell Culture Rimonabant Experiment

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Cell culture materials were purchased from GIBCO (Life Technologies, USA). Rimonabant was purchased from TOCRIS (Tocris Cookson, UK). All other chemicals, unless otherwise stated, were from Sigma (USA).

Chen B, & Hu N. (2017). Rimonabant improves metabolic parameters partially attributed to restoration of high voltage-activated Ca2+ channels in skeletal muscle in HFD-fed mice. Brazilian Journal of Medical and Biological Research, 50(6), e6141.

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Pharmacological Modulation of TRP and CB Receptors

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THC (Dronabinol) was obtained from THC Pharm, Frankfurt, Germany. A967079, COR170, HC030031, ruthenium red (RR) and rimonabant were obtained from Tocris/Biotechne, Wiesbaden, Germany. 1,1′-Diethyl-2,2′-cyanine iodide (Decynium-22; D22) was obtained from Sigma Aldrich, Taufkirchen, Germany. THC is a partial agonist at CB₁ and CB₂ receptors, but also at several TRP channels. It is the major psychoactive constituent of the plant cannabis sativa. A967079 and HC030031 are lipophilic antagonists at the TRPA1 ion channel, whereas RR unselectively blocks TRP channels at the plasma membrane. rimonabant and COR170 are inverse agonists at CB₁ and CB₂, respectively. D22 is an inhibitor of organic cation transporters and monoamine transport.

Lowin T., Kok C., Smutny S, & Pongratz G. (2022). Impact of Δ9-Tetrahydrocannabinol on Rheumatoid Arthritis Synovial Fibroblasts Alone and in Co-Culture with Peripheral Blood Mononuclear Cells. Biomedicines, 10(5), 1118.

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Olanzapine and Angiotensin II Protocol

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Olanzapine (PubChem CID: 4585) was a generous gift of Neuland Laboratories Ltd (Hyderabad, India). Angiotensin II (PubChem CID: 172198) and rimonabant (RIM; PubChem CID: 104850) were purchased from Tocris Bioscience (Minneapolis, Minnesota). Angiotensin II was used at the end of the experiment to verify that the intracerebroventricular (ICV) cannulas were placed correctly by performing a water drinking test.

Klingerman C.M., Stipanovic M.E., Hajnal A, & Lynch C.J. (2015). Acute Metabolic Effects of Olanzapine Depend on Dose and Injection Site. Dose-Response, 13(4), 1559325815618915.

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Cannabinoid Receptor Ligand Pharmacology

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CP-55,940, rimonabant, and AM-630 were obtained from Tocris Bioscience (Bristol, UK). All drugs were prepared as a stock solution in 100% DMSO at a concentration of 100 mM, divided into aliquots, and maintained at −4 °C until use. [³H]CP-55,950 (168 Ci/mmol) and [³⁵S]GTPγS (1250 Ci/mmol) were purchased from Perkin Elmer (Boston, MA). All other reagents were obtained from Fisher Scientific Inc. (Pittsburgh, PA).

Fulo H.F., Shoeib A., Cabanlong C.V., Williams A.H., Zhan C.G., Prather P.L, & Dudley G.B. (2021). Synthesis, Molecular Pharmacology, and Structure–Activity Relationships of 3-(Indanoyl)indoles as Selective Cannabinoid Type 2 Receptor Antagonists. Journal of medicinal chemistry, 64(9), 6381-6396.

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Immunomodulatory Effects of Cannabinoids

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We used RPMI 1640 medium (Lonza) supplemented (cRPMI) with 10% heat‐inactivated fetal bovine serum (FBS, Hyclone) 100 μg/mL normocin (InvivoGen), 50 μg/mL penicillin‐streptomycin, 1% nonessential amino acids, 1% MEM vitamins and 1 mmol/L sodium pyruvate (all from Life Technologies). Lipopolysaccharide (LPS) from Escherichia coli O127:B8 and O155:B5 (Sigma–Adrich) were used for cell cultures and animal models, respectively. Pam3CSK4 (InvivoGen) as TLR2 ligand and a combination of TNFα and IL-1β (both from PeproTech) as a maturing factors (MFs) were used. CBR agonists WIN55212-2 (Sigma Aldrich) and HU210, selective CB2 agonist HU308, selective antagonists for CB1 (Rimonabant), CB2 (AM630), PPARα (GW6471), PPARγ (GW9662) (all from Tocris) and autophagy inhibitor 3-methyladenine (3-MA; InvivoGen) were used.

Angelina A., Pérez-Diego M., López-Abente J., Rückert B., Nombela I., Akdis M., Martín-Fontecha M., Akdis C, & Palomares O. (2021). Cannabinoids induce functional Tregs by promoting tolerogenic DCs via autophagy and metabolic reprograming. Mucosal Immunology, 15(1), 96-108.

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