Nod scid gamma mice

Manufactured by Jackson ImmunoResearch

Sourced in United States, Montenegro

NOD scid gamma (NSG) mice are an immunodeficient mouse model developed by Jackson ImmunoResearch. These mice lack mature T cells, B cells, and natural killer cells, making them a valuable tool for research involving human cell and tissue transplantation.

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Lab products found in correlation

Matrigel, by Corning (9 mentions) Matrigel, by BD (5 mentions) Mda mb 231, by American Type Culture Collection (3 mentions) Cb17 scid mice, by Taconic Biosciences (2 mentions) Electronic digital caliper, by Thermo Fisher Scientific (2 mentions) Nod scid, by Jackson ImmunoResearch (2 mentions) D luciferin luciferase, by PerkinElmer (2 mentions) Vivoglo caspase 3 7 substrate z devd aminoluciferine sodium salt, by Promega (2 mentions) Living image, by PerkinElmer (2 mentions) Epilife medium, by Thermo Fisher Scientific (2 mentions) C57bl 6j, by Jackson ImmunoResearch (2 mentions) Mda mb 231, by Thermo Fisher Scientific (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Cd 1 nude mice, by Charles River Laboratories (2 mentions) Vevo 3100, by Fujifilm (2 mentions) Mgfap cre line 77, by Jackson ImmunoResearch (2 mentions) Wild type male c57 bl6j, by Jackson ImmunoResearch (2 mentions) Ng2 cre36, by Jackson ImmunoResearch (2 mentions) Rosa tdtomato tdt 37, by Jackson ImmunoResearch (2 mentions) Rosa yfp68, by Jackson ImmunoResearch (2 mentions)

Spelling variants of this product

NOD/SCID mice (263 citations) NOD/SCID (81 citations) NOD mice (59 citations) SCID mice (40 citations) NOD-SCID gamma (NSG) female mice (12 citations) NOD/SCID gamma male mice (9 citations) NOD/SCID gamma mice (NSG; (6 citations) NOD-SCID IL2 receptor gamma chain knockout (NSG) mice (4 citations) NOD Scid gamma (NSG; NOD-SCID) mice (2 citations) NOD SCID gamma chain knockout (NSG) mice (2 citations)

168 protocols using nod scid gamma mice

Lung Colonization and Xenograft Assays in NOD/SCID Mice

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Seven- to twelve-week-old age-matched NOD/SCID gamma mice (Jackson Labs) were used for lung colonization assays. Six- to eight-week-old NOD/SCID gamma mice (Jackson Labs) were used for lung xenograft experiments. Male mice were used for assays with H1299 cells, and female mice were used for assays with H1975 cells. Mice were randomly assigned to experimental cohorts. Specific-pathogen-free conditions and facilities were approved by the American Association for Accreditation of Laboratory Animal Care. Surgical procedures were reviewed and approved by the UCSF Institutional Animal Care and Use Committee (IACUC), protocol #AN107889–03 and protocol #AN179718.

Fish L., Navickas A., Culbertson B., Xu Y., Nguyen H.C., Zhang S., Hochman M., Okimoto R., Dill B.D., Molina H., Najafabadi H.S., Alarcón C., Ruggero D, & Goodarzi H. (2019). Nuclear TARBP2 drives oncogenic dysregulation of RNA splicing and decay. Molecular cell, 75(5), 967-981.e9.

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Xenograft Tumor Induction in NOD scid Gamma Mice

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NOD scid gamma mice were obtained from The Jackson Laboratory. All mice used in the study were 6 weeks of age. Mouse care followed the guidelines of the Animal Care Committee of the Korea Research Institute of Bioscience and Biotechnology. The Dsg2-positive or -negative cells were dissociated using Accutase and the pellets were suspended in 1:1 (v/v) DMEM/F12 and Matrigel. Approximately 1 cm of abdominal skin was incised with fine scissors under isoflurane inhalation anesthesia. The testes were removed from the abdominal cavity using sterilized forceps, and cells were injected into each side of the testes using a 31-gauge Ultra-Fine syringe. See Supplemental Experimental Procedures for additional experimental information and further procedures.

Park J., Son Y., Lee N.G., Lee K., Lee D.G., Song J., Lee J., Kim S., Cho M.J., Jang J.H., Lee J., Park J.G., Kim Y.G., Kim J.S., Lee J., Cho Y.S., Park Y.J., Han B.S., Bae K.H., Han S., Kang B., Haam S., Lee S.H., Lee S.C, & Min J.K. (2018). DSG2 Is a Functional Cell Surface Marker for Identification and Isolation of Human Pluripotent Stem Cells. Stem Cell Reports, 11(1), 115-127.

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Xenograft Mouse Model Establishment

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BALB/c nude, NCr nude or NOD-scid-gamma mice were purchased from the InVivos, Singapore or Jackson Laboratories (Bar Harbor, ME, USA). The Duke-NUS Institutional Animal Care and Use Committee or BRC Institutional Animal Care and Use Committee approved all animal studies. Animals were housed in standard cages and were allowed access ad libitum to food and water.

Madan B., Ke Z., Harmston N., Ho S.Y., Frois A.O., Alam J., Jeyaraj D.A., Pendharkar V., Ghosh K., Virshup I.H., Manoharan V., Ong E.H., Sangthongpitag K., Hill J., Petretto E., Keller T.H., Lee M.A., Matter A, & Virshup D.M. (2015). Wnt addiction of genetically defined cancers reversed by PORCN inhibition. Oncogene, 35(17), 2197-2207.

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Lung Colonization Assay in Mice

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Seven- to twelve-week-old age-matched NOD/SCID gamma mice (Jackson Labs) were used for lung colonization assays. Male mice were used for assays with H1299 cells, and female mice were used for assays with H1975 cells. In all cases, 5×10⁴ cancer cells constitutively expressing luciferase were suspended in 100 μL PBS and then injected via tail-vein. Each cohort contained 4–5 mice. Cancer cell growth was monitored in vivo at the indicated times by retro-orbital injection of 100ul of 15mg/mL luciferin (Perkin Elmer) dissolved in 1X PBS, and then measuring the resulting bioluminescence with an IVIS instrument and Living Image software (Perkin Elmer).

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Immunohistochemical Analysis of PDPN in OSCC

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Surgical specimens were fixed in 10% formalin in PBS, paraffin embedded, sectioned (4 microns), and processed for hematoxylin/eosin staining and immunohistochemistry with 8.1.1 and D2-40 monoclonal antibodies (Dako) to detect mouse and human PDPN, respectively, as described [61 , 106 (link), 107 (link)]. OSCC cells were cultured in chamber slides (Lab-Tek 177445), fixed in 10% formalin, and processed for immunohistochemistry as described above. For mouse xenograft studies, 1 million HSC-2 cells were injected into the left flank of immunodeficient NOD scid gamma mice (Jackson Labs 005557) and allowed to form tumors which were excised and examined by immunohistochemistry. Human and mouse experimental protocols were approved by the University Institutional Review Board (study ID Pro2012001544) and Institutional Animal Care and Use Committee (APR 10579), respectively.

Ochoa-Alvarez J.A., Krishnan H., Pastorino J.G., Nevel E., Kephart D., Lee J.J., Retzbach E.P., Shen Y., Fatahzadeh M., Baredes S., Kalyoussef E., Honma M., Adelson M.E., Kaneko M.K., Kato Y., Young M.A., Deluca-Rapone L., Shienbaum A.J., Yin K., Jensen L.D, & Goldberg G.S. (2015). Antibody and lectin target podoplanin to inhibit oral squamous carcinoma cell migration and viability by distinct mechanisms. Oncotarget, 6(11), 9045-9060.

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Xenograft Tumor Model in NOD/SCID Mice

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The animal studies and procedures were approved by the Association for Assessment and Accreditation of Laboratory Animal Care-accredited Institutional Animal Care and Use Committees of Veteran’s Administration Pittsburgh Health System. Seven-week-old male NOD/SCID gamma mice (Jackson Laboratory) were anesthetized with ketamine/xylazine. Long-acting buprenorphine was used as pain reliever. Sterile surgery was performed to expose the spleen. Half a million of cancer cells were injected into the spleen using 27-gauge needle. Running stitch with absorbable suture was performed to close the omentum and metal wound clips were used to close the skin wound. Mice were sacrificed after 5 weeks using a carbon dioxide chamber according to AVMA Guidelines on Euthanasia.

Ma B., Khazali A., Shao H., Jiang Y, & Wells A. (2019). Expression of E-cadherin and specific CXCR3 isoforms impact each other in prostate cancer. Cell Communication and Signaling : CCS, 17, 164.

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B-like Hodgkin Lymphoma Combination Therapy

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Example 8

In Vivo Combination Treatment of cAC10-vcE and Everolimus in B-Like Hodgkin Lymphoma

To test combination treatments in B-like Hodgkin lymphoma in vivo, a murine xenograft model was used. L428 cells were implanted into NOD-SCID-gamma mice (The Jackson Laboratory) to generate tumors. Tumors were grown to an average of 90 mm³, then sorted into groups of 10 mice. Mice were not treated, treated with everolimus (15 mg/kg), treated with cAC10 (1 mg/kg), treated with cAC10-vcE (1.0 mg/kg), treated with cAC10 and everolimus, or treated with cAC10-vcE and everolimus. The cAC10-vcE and cAC10 was administered q4d×4 ip. The everolimus was administered q1d×14 po. At day 61, the cAC10-vcE and everolimus combination treatment arm has 10/10 complete responses and the cAC10-vcE and everolimus single treatment arms had no complete responses.

US10201615B2. Synergistic effects between auristatin-based antibody drug conjugates and inhibitors of the PI3K-AKT mTOR pathway (2019-02-12). Seattle Genetics, Inc. [US]. Inventors: Timothy S. Lewis [US], Che-Leung Law [US], Julie A. McEarchern [US].

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Xenograft Mouse Model for Tumor Growth

Cited 1 time

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All animal work was performed in accordance with protocols approved by the University of Cincinnati Standing Committees on Animals. Female nude CD-1 mice (Charles River) (A549 cell line), NOD/SCID-gamma mice (Jackson Lab) (LAM 621-101 cell line), and CB17-scid mice (Taconic) (RT4 and ELT3-luciferase cell lines), 6 to 8 weeks of age, were used. 2×10⁶ cells were subcutaneously inoculated into the posterior back regions of each mouse. Once the tumor is formed three to five weeks post inoculation, tumor length and width were measured using a digital caliper. Tumor volume was calculated using the formula: volume = (length × (width)²/2). For the drug treatment experiment, mice were randomized into two groups after tumor formation and treated with either vehicle control (100 µl of 1% DMSO in PBS) or SRPIN340 (100 µl of 20 µg/ml; 1% DMSO in PBS) by daily peritumoral injection as previously described (Gammons et al., 2014 (link)). SRPIN340 was dissolved as 2 mg/ml in DMSO, and this stock solution was diluted 1:1,000 using PBS prior to inject. For the bioluminescent imaging of ELT3-luciferase tumors, luciferin (120 mg/kg, Xenogen) was intraperitoneally injected into the mouse 10 min prior to imaging. Bioluminescent signals were recorded using the Xenogen IVIS System and the total photon flux of tumors was analyzed as previously described (Yu et al., 2009 (link)).

Lee G., Zheng Y., Cho S., Jang C., England C., Dempsey J.M., Yu Y., Liu X., He L., Cavaliere P.M., Chavez A., Zhang E., Isik M., Couvillon A., Dephoure N.E., Blackwell T.K., Yu J.J., Rabinowitz J.D., Cantley L.C, & Blenis J. (2017). Post-transcriptional regulation of de novo lipogenesis by mTORC1-S6K1-SRPK2 signaling. Cell, 171(7), 1545-1558.e18.

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In Vivo Animal Studies Protocol

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Animal studies were approved by the Animal Care and Use Committee of MD
Anderson Cancer Center. Female Nod.Scid gamma mice, age 4 to 6 weeks old
(Jackson Laboratories, ME), were used for all of the in vivostudies. Mice were housed under pathogen-free conditions and treated in
accordance with NIH guidelines.

Lee J., Lim B., Pearson T., Choi K., Fuson J.A., Bartholomeusz C., Paradiso L.J., Myers T., Tripathy D, & Ueno N.T. (2019). Anti-tumor and anti-metastasis efficacy of E6201, a MEK1 inhibitor, in preclinical models of triple-negative breast cancer. Breast cancer research and treatment, 175(2), 339-351.

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Evaluating Anti-Tumor Effects of Cuc D

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We performed ectopic xenograft studies in mice to determine the anti-tumor effect of Cuc D. To this end, six-week old NOD-SCID gamma mice were purchased from Jackson laboratory and maintained in a pathogen-free environment. All of the procedures were carried out in accordance with the protocol that the UTHSC Institutional Animal Care and Use Committee (UTHSC-IACUC) approved. HPAF-II cells (4 × 10⁶ cells) were suspended in phosphate buffer saline (PBS) and Matrigel (BD Biosciences) solution (1:1 ratio) and subcutaneously injected on the dorsal flanks of each mouse to establish ectopic xenograft tumors in mice. Mice tumor growth was monitored while using a digital Vernier caliper. When tumor volume reached ~100 mm³, mice were divided into control groups and Cuc D treatment groups. The mice were treated with Cuc D (1 mg/kg bwt thrice a week; intra-peritoneally) or vehicle control (PBS). Tumor volumes were measured weekly and then calculated while using formula 0.5238 × L × W × H, where L is length, W is width, and H is tumor height. Mice were euthanized when the control mice tumor volume reached ~1000 mm³. The tumors were excised and processed for RNA, tissue lysates, histopathology, and preparation of slides (5μm section) at the time of sacrifice.

Sikander M., Malik S., Khan S., Kumari S., Chauhan N., Khan P., Halaweish F.T., Chauhan B., Yallapu M.M., Jaggi M, & Chauhan S.C. (2019). Novel Mechanistic Insight into the Anticancer Activity of Cucurbitacin D against Pancreatic Cancer (Cuc D Attenuates Pancreatic Cancer). Cells, 9(1), 103.

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