FT-IR spectra were obtained from the KBr slice with a Nicolet iS10 FT-IR spectrophotometer (Thermo Fisher Scientific, Shanghai). X-ray diffraction (XRD) patterns were recorded on a Bruker D8 Advance diffractometer (Germany) with a Cu Kα (1.5406 Å) radiation source. All pH measurements were performed with a Sartorius PB-10 digital pH meter (Shanghai, China). Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) were performed with a HITACHI model HT7700 instrument operating at 80 kV accelerating voltage and a ZEISS Gemini 300 with OXFORD Xplore, respectively. The ultraviolet-visible (UV-Vis) absorption spectra were recorded by using a U3900 spectrophotometer (Hitachi, Japan). All fluorescence measurements were performed on a Hitachi F-7000 fluorescence spectrometer. The excitation wavelength was set at 496 nm, and the emission spectra from 550 to 750 nm were observed. The fluorescence intensity at 610 nm was used to evaluate the performance of the proposed strategy.
U 3900 spectrophotometer
Manufactured by Hitachi
Sourced in Japan, China
The Hitachi U-3900 spectrophotometer is a compact and versatile instrument designed for a wide range of UV-Vis absorption measurements. It features a dual-beam optical system, a wavelength range from 190 to 1100 nm, and high-resolution scanning capabilities. The U-3900 provides accurate and reliable data for various applications in the fields of chemistry, biology, and materials science.
Automatically generated - may contain errors
Lab products found in correlation
Cpl 200 spectrophotometer, by Jasco (5 mentions)
F 7000 fluorescence spectrometer, by Hitachi (4 mentions)
Zetasizer nano z, by Malvern Panalytical (4 mentions)
Dm2700m upright materials microscope, by Leica camera (3 mentions)
Tecnai g2 f20, by Thermo Fisher Scientific (3 mentions)
D8 advance, by Bruker (3 mentions)
Zetasizer nano zs90, by Malvern Panalytical (3 mentions)
Pb 10 digital ph meter, by Sartorius (2 mentions)
Jem 2100, by Thermo Fisher Scientific (2 mentions)
Omin λ500i monochromator, by Zolix (2 mentions)
J 1500, by Jasco (2 mentions)
D8 diffractometer, by Bruker (2 mentions)
Nicolet is10 spectrometer, by Thermo Fisher Scientific (2 mentions)
S 4800, by Hitachi (2 mentions)
Escalab 250xi, by Thermo Fisher Scientific (2 mentions)
Nicolet is10 ftir spectrophotometer, by Thermo Fisher Scientific (1 mentions)
Jem 2100f electron microscope, by JEOL (1 mentions)
Jem 2010 transmission electron microscope, by JEOL (1 mentions)
Fluoromax 4 fluorescence spectrophotometer, by Horiba (1 mentions)
Fls980 spectrometer, by Edinburgh Instruments (1 mentions)
69 protocols using u 3900 spectrophotometer
1
Multifunctional Material Characterization
2
Characterization of Nanoparticle Structures
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Transmission electron microscopy (TEM) and high resolution TEM (HRTEM) were performed on the JEOL JEM-2100F electron microscope operating at 200 kV. For the TEM measurements, a drop of the nanoparticle solution was dispensed onto a 3-mm carbon-coated copper grid. Excessive solution was removed by an absorbent paper, and the sample was dried under vacuum at room temperature. An energy dispersive X-ray spectroscopy (EDX) analyzer attached to the TEM was used to analyze the chemical compositions of the synthesized nanoparticles. UV-visible spectra of the core-shell and CBS particle solutions were collected on a Hitachi U-3900 spectrophotometer. X-ray photoelectron spectroscopy (XPS) was conducted on a VG ESCALAB MKII spectrometer. Sample preparation for XPS analysis began with concentrating 5 mL of the toluene solution of the metal nanoparticles to 0.5 mL using flowing N2. 10 mL of methanol was then added to precipitate the metal nanoparticles. The nanoparticles were then recovered by centrifugation and washed with methanol several times to remove non-specifically bound oleylamine. The nanoparticles were then dried at room temperature in vacuum.
3
Degree of Hydrolysis Analysis by OPA
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The degree of hydrolysis was analyzed by the O-phthalaldehyde (OPA) method [16 (link)] with some modifications. Briefly, OPA reagent was prepared by dissolving 7.620 g sodium tetraborate (CAS 1303-96-4) and 200 mg sodium dodecyl sulfate (SDS) in 150 ml of deionized water. Then 160 mg O-phthalaldehyde (OPA, purity 97%) dissolved in 4 ml absolute ethanol was added, followed by addition of 176 mg 1,4-dimercaptothreitol (DTT, purity 99%), and filling deionized water till total volume of 200 ml. Each 400 μl sample solution was mixed with 3 mLOPA reagent, and the absorbance was measured at 340 nm by a U-3900 Spectrophotometer (Hitachi, Japan). The calculation formula is as follows: DH (%) = h/htot×100%, where htot is the total number of peptide bonds per gram of protein and h is the total number of peptide bonds per gram of hydrolyzed protein, and h = (SerineNH2 − β)/α, where α, β, and htot are 1.039, 0.383, and 8.2 mEq/g protein in casein, respectively.
4
Comprehensive Characterization of Materials
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TEM and HRTEM images were obtained with a JEOL JEM‐2010 transmission electron microscope. XRD patterns were carried out by X‐ray diffraction using Cu Kα radiation (Rigaku‐D/Max 2500). Raman spectra were recorded using a HORIB A Evolution Raman spectrometer. FTIR spectra were collected by a TENSOR2 spectrometer. XPS curves were obtained on an AXIS ULTRA DLD X‐ray diffractometer. UV–vis spectra were recorded using a HITACHI U‐3900 spectrophotometer. The PL spectra were collected on the HORIBA FluoroMax‐4 fluorescence spectrophotometer. The TRPL curves were measured on an EDINBURGH FLS980 spectrometer. The photoelectric properties of the LEDs were measured by Spectrascan PR655. The thermogravimetric curve was measured using a Setaram Labsys Evo thermogravimetric analyzer with a heating rate of 10 °C min−1 in a nitrogen atmosphere.
5
Spectrophotometric Determination of Chlorophyll a and Phycocyanin in C. merolae
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C. merolae cultures were prepared at an OD750 of 0.2–0.8. OD750 was measured using a UV-visible DU730 spectrophotometer (Beckman Coulter, Brea, CA). Absorbance was measured continuously at 700–400 nm, using a U-3900 spectrophotometer (Hitachi, Tokyo, Japan). Aluminum oxide 210-0740 (Hitachi High-Tech Science, Tokyo, Japan) was used as the subwhite plate. From the absorbance values, cellular Chl a and phycocyanin levels were determined, as follows (Arnon et al., 1974 (link)):
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C. merolae cultures were prepared at an OD750 of 0.2–0.8. OD750 was measured using a UV-visible DU730 spectrophotometer (Beckman Coulter, Brea, CA). Absorbance was measured continuously at 700–400 nm, using a U-3900 spectrophotometer (Hitachi, Tokyo, Japan). Aluminum oxide 210-0740 (Hitachi High-Tech Science, Tokyo, Japan) was used as the subwhite plate. From the absorbance values, cellular Chl a and phycocyanin levels were determined, as follows (Arnon et al., 1974 (link)):
6
Photometric Monitoring of Chlorite Conversion
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The conversion of chlorite (ClO2–) into
chloride and dioxygen was monitored photometrically by following the
decrease in absorbance at 260 nm (ε260 = 155 M–1 s–1)18 (link) on a Hitachi U-3900 spectrophotometer. Reactions were conducted
in 50 mM buffer solutions (pH 5.5 and 7.0). The chlorite concentration
was 340 μM, and reactions were started by the addition of the
enzyme.
chloride and dioxygen was monitored photometrically by following the
decrease in absorbance at 260 nm (ε260 = 155 M–1 s–1)18 (link) on a Hitachi U-3900 spectrophotometer. Reactions were conducted
in 50 mM buffer solutions (pH 5.5 and 7.0). The chlorite concentration
was 340 μM, and reactions were started by the addition of the
enzyme.
7
Photophysical Characterization of Materials
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UV–vis and fluorescence spectra were recorded on the Hitachi U‐3900 spectrophotometer and F‐4500 fluorescence spectrophotometer, respectively. CPL spectra were measured on JASCO CPL‐200 spectrophotometers. Lifetime measurements were recorded on the spectrometer using time‐correlated single‐photon counting. POM images were recorded on Leica DM2700M upright materials microscope. XRD spectra were measured on the Rigaku D/Max‐2500 X‐ray diffractometer (Japan) with Cu/Kα radiation (λ = 1.5406 Å). The photoreversion reactions were conducted by irradiation with 465 nm blue LED (HLV2‐ 22BL‐3W, CCS Inc.) or UV 365 nm.
8
Catalase, Peroxidase, and Chlorite Dismutase Activities
9
Synthesis and Characterization of Stellated Ag-Pt Nanoparticles
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TEM, HRTEM, and STEM were performed on the JEOL JEM-2100 and FEI Tecnai G2 F20 electron microscope operating at 200 kV with a supplied software for automated electron tomography. For the TEM measurements, a drop of the nanoparticle solution was dispensed onto a 3-mm carbon-coated copper grid. Excessive solution was removed by an absorbent paper, and the sample was dried under vacuum at room temperature. An EDX analyzer attached to the TEM operating in the scanning transmission electron microscopy (STEM) mode was used to analyze the chemical compositions of the synthesized nanoparticles. UV-visible spectra of the reaction system at different time during the synthesis of stellated Ag-Pt bimetallic nanoparticles were collected on a Hitachi U-3900 spectrophotometer. X-ray photoelectron spectroscopy (XPS) was conducted on a VG ESCALAB MKII spectrometer. Powder XRD patterns were recorded on a Rigaku D/Max-3B diffractometer, using Cu Kα radiation (λ = 1.54056 Å). Samples for XPS and XRD analyses were concentrated from the toluene solution of nanoparticles to 0.5 mL using flowing N2. 10 ml of methanol was then added to precipitate the nanoparticles, which were recovered by centrifugation, washed with methanol several times, and then dried at room temperature in vacuum.
10
Comprehensive Characterization of Material Samples
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Transmission electron microscopy (TEM) images were obtained on a JEM-2100 microscope at an acceleration voltage of 200 kV. Morphologies of samples were characterized using scanning electron microscopes (FE-SEM, S-4800, an acceleration voltage of 10 kV, Hitachi High-technologies, Japan) with an energy-dispersive X-ray (EDX) acceleration voltage analyzer. X-ray diffraction (XRD) patterns was obtained from a Bruker D8 Advance X-ray diffractometer (Bruker, USA) with Cu-Kα radiation (λ = 1.5406 Å) at a scanning rate of 10° min−1 and a scanning range from 10° to 90°. X-ray photoelectron spectroscopy (XPS) measurement was carried out with an ESCALab220i-XL spectrometer using a twin-anode Al-Kα X-ray source (1486.6 eV). Micro-Raman spectra were achieved from a Raman Spectroscope (Renishaw inVia plus, United Kingdom) under ambient conditions with 514 nm excitation from an argon ion laser. Ultraviolet-visible (UV-vis) data were acquired with a U-3900 spectrophotometer (Hitachi, Ltd., Japan). Ultraviolet-visible-infrared (UV-vis-IR) diffuse reflectance spectra were recorded at room temperature on an Agilent Cary 500 UV-vis-IR Spectrometer equipped with an integrating sphere using BaSO4 as a reference. The photoluminescence spectra were obtained using a Horiba Jobin Yvon FluoroLog3 spectrometer.
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