Streptomycin sulfate

Manufactured by Sangon

Sourced in China

Streptomycin sulfate is a white to off-white powder that is a broad-spectrum antibiotic derived from the bacterium Streptomyces griseus. It is a common lab reagent used in microbiological and cell culture applications.

Automatically generated - may contain errors

Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (7 mentions) Penicillin, by Sangon (7 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (4 mentions) Rpmi 1640, by Thermo Fisher Scientific (3 mentions) Dulbecco modified eagle medium (dmem), by Solarbio (2 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions) Ionomycin, by Absin (1 mentions) Penicillin g, by Sangon (1 mentions) Amphotericin b, by Sangon (1 mentions) Brefeldin a, by BD (1 mentions) 1640 medium, by Solarbio (1 mentions) B540732, by Sangon (1 mentions) Hek293t cell, by American Type Culture Collection (1 mentions) Marc 145 cells, by American Type Culture Collection (1 mentions) Fetal bovine serum (fbs), by Sartorius (1 mentions)

9 protocols using streptomycin sulfate

Porcine Cell Lines and Pseudorabies Virus

Cited 2 times

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Porcine kidney epithelial PK-15 (CCL-33, ATCC), Vero (CRL-1586, ATCC), porcine alveolar macrophages (PAM [17 (link)]), and HEK293T (CRL-11268, ATCC) cells were grown at 37 ℃ with 5% CO₂ in DMEM (12110, Solarbio) supplemented with 10% foetal bovine serum (FBS, A5669701, Gibco), 100 U/mL penicillin and 100 μg/mL streptomycin sulfate (B540732, Sangon).
The virulent PRV isolate QXX (PRV-QXX) was kindly donated by Yong-Tao Li from the College of Veterinary Medicine, Henan Agricultural University [18 (link)]. The recombinant PRV strain PRV-GFP was derived from the PRV Hubei strain, with the TK gene replaced by a GFP expression cassette from the pEGFP-N1 plasmid [19 (link)].
Full-length porcine Rab6 cDNA was amplified by polymerase chain reaction (PCR). Rab6 cDNA was cloned and inserted into p3 × FLAG-CMV-10 expression plasmids to generate FLAG-Rab6, FLAG-Rab6 Q72L and FLAG-Rab6 T27N. All plasmids were transfected with Lipofectamine 3000 (L3000015, Invitrogen) according to the manufacturer’s instructions. The primer sequences used for gene amplification were as follows: Rab6-Fw: 5ʹ-ATGTCCACGGGCGGAGAC-3ʹ; Rab6-Rv: 5ʹ-TTAGCAGGAACAGCCTCCTTCA-3ʹ; Rab6 Q72L-Fw: 5ʹ-AGGTCTAGAGCGGTTCAGGAGCTTGATTCCTA-3ʹ; Rab6 Q72L-Rv: 5ʹ-TGAACCGCTCTAGACCTGCTGTGTCCCATAATTGC-3ʹ; Rab6 T27N-Fw: 5ʹ-GCGTTGGAAAGAACTCTTTGATCACCAGATTCATGTATGA-3ʹ; Rab6 T27N-Rv: 5ʹ-AAGAGTTCTTTCCAACGCTTTGCTCCCCCAGGA-3ʹ.

Liang D.G., Guo Y.K., Zhao S.B., Yang G.Y., Han Y.Q., Chu B.B, & Ming S.L. (2024). Pseudorabies virus hijacks the Rab6 protein to promote viral assembly and egress. Veterinary Research, 55, 68.

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In Vitro Activation of T Cells

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T cell culture medium was RPMI 1640 (#11875119, Gibco) supplemented with heat-inactivated fetal bovine serum (10%; #SH30084.03, Hyclone), penicillin G (100U/ml; #B540733, Sangon), streptomycin sulfate (100 μg/ml; #B540733, Sangon) and amphotericin B (2.5 μg/ml; #B540733, Sangon). Mouse skin-draining axillary, brachial and inguinal lymph nodes (LN) were harvested. LN single-cell suspensions were prepared through grinding by glass slides and filtering through a 40 μm cell strainer. LN and dermal cells were cultured in T cell culture medium in the presence of phorbol-12-myristate-13-acetate (PMA; 50 ng/ml; #P1585, Sigma), ionomycin (1mM; #abs42019871, Absin) and brefeldin A (1:1000; #347688, BD) for 4 h.

Zhang X., Li X., Chen Y., Li B., Guo C., Xu P., Yu Z., Ding Y., Shi Y, & Gu J. (2021). Xiao-Yin-Fang Therapy Alleviates Psoriasis-like Skin Inflammation Through Suppressing γδT17 Cell Polarization. Frontiers in Pharmacology, 12, 629513.

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Cell Culture and Viral Infection Protocols

Cited 4 times

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Pig kidney (PK-15) cells (CCL-33, ATCC), human cervical cancer (HeLa) cells (CL-82, ATCC), human embryonic kidney (HEK293) cells (CRL-1573, ATCC), HEK293 cells transformed with SV40 large T antigen (HEK293T) (CRL-11268, ATCC), mouse fibroblast (NIH/3T3) cells (CRL-1658, ATCC), African green monkey kidney (Vero) cells (CL-81, ATCC), human lung carcinoma (A549) cells (CCL-185, ATCC), mouse macrophages (RAW264.7) (TIB-71, ATCC) and canine kidney (MDCK) cells (CCL-34, ATCC) were grown in monolayers at 37°C under 5% CO₂.
All cells were cultured in DMEM (10566–016, GIBCO) supplemented with 10% FBS (10099141C, GIBCO), 100 units/mL penicillin and 100 μg/mL streptomycin sulfate (B540732, Sangon). African green monkey kidney (MARC-145) cells and cGAS^-/-, STING^-/-, TBK1^-/-, IRF3^-/-, IFNAR1^-/- PK15 cells were cultivated as previously described [72 (link), 73 (link)].
PRV-GFP, PRV-QXX and PRRSV-BJ4 were used as previously described [57 (link), 72 (link), 74 (link)]. VSV-GFP, NDV-GFP and H1N1-PR8 were gifts from Yong-Tao Li (Henan Agricultural University, China) [75 (link)]. HSV-F was a gift from Chun-Fu Zheng (Fujian Medical University, China) [76 (link)]. ECTV was a gift from Han-Zhong Wang (Wuhan Institute of Virology, Chinese Academy of Sciences) [77 (link)].

Wang J., Li G.L., Ming S.L., Wang C.F., Shi L.J., Su B.Q., Wu H.T., Zeng L., Han Y.Q., Liu Z.H., Jiang D.W., Du Y.K., Li X.D., Zhang G.P., Yang G.Y, & Chu B.B. (2020). BRD4 inhibition exerts anti-viral activity through DNA damage-dependent innate immune responses. PLoS Pathogens, 16(3), e1008429.

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Cell Culture Protocols for Genetic Manipulation

Cited 1 time

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The 3D4/21 (ATCC, CRL-2843), PK-15 (ATCC, CCL-33), and HeLa (ATCC, CCL-2) cells were cultured in DMEM (Gibco, Waltham, MA, USA) supplemented with 10% FBS (Gibco), 100 units/mL penicillin, and 100 mg/mL streptomycin sulfate (Sangon, Shanghai, China). All cells were grown in monolayers at 37 °C in 5% CO₂. The 3D4/21 P65^−/−, PK-15 ATG5^−/−, and Beclin-1^−/− cells were used and cultivated as previously described [19 (link),20 (link)].

Wang Q., Zhou L., Wang J., Su D., Li D., Du Y., Yang G., Zhang G, & Chu B. (2022). African Swine Fever Virus K205R Induces ER Stress and Consequently Activates Autophagy and the NF-κB Signaling Pathway. Viruses, 14(2), 394.

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Porcine Cell Lines and Virus Strains

Cited 1 time

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Porcine kidney epithelial PK-15 (CCL-33, ATCC), porcine alveolar macrophages 3D4/21 (CRL-2843, ATCC), and human cervical cancer HeLa (CL-82, ATCC) cells were grown in monolayers at 37 °C under 5% CO₂ in DMEM (Gibco, Waltham, MA, USA) supplemented with 10% FBS (Gibco), 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate (Sangon, Shanghai, China). Virus titers were determined by the 50% tissue culture infective dose (TCID₅₀) assay, which was calculated with the Reed–Muench method.
The virulent PRV isolate QXX (PRV-QXX) was kindly donated by Yong-Tao Li from the College of Veterinary Medicine, Henan Agricultural University [20 (link)]. The recombinant PRV strain of PRV-GFP, derived from the PRV Hubei strain with the TK gene replaced by a GFP expression cassette from the pEGFP-N1 plasmid, was kindly donated by Han-Zhong Wang from Wuhan Institute of Virology, Chinese Academy of Sciences [21 (link)].
Full-length porcine AP2B1 cDNA was cloned into the mCherry-N1 expression plasmid using the BamHI and KpnI restriction sites.

Wang Y., Li G.L., Qi Y.L., Li L.Y., Wang L.F., Wang C.R., Niu X.R., Liu T.X., Wang J., Yang G.Y., Zeng L, & Chu B.B. (2022). Pseudorabies Virus Inhibits Expression of Liver X Receptors to Assist Viral Infection. Viruses, 14(3), 514.

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Cell Culture and Viral Strains Protocol

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MARC-145, HEK-293T, HeLa, and CRL-2843-CD163 cell lines were stored in our laboratory (71 (link)). MARC-145 and HEK-293T cells were maintained in DMEM (Solarbio, Cat. No. 12100) supplemented with 10% heat-inactivated FBS (Gibco, Cat. No. 10270–106), and 100 units mL⁻¹ penicillin and 100 mg mL⁻¹ streptomycin sulfate (Sangon, Cat. No. B540732) at 37°C in 5% CO₂. HeLa cells were routinely maintained in modified Eagle medium (iCell Bioscience Inc., Cat. No. 138–0012) supplemented with 10% heat-inactivated FBS and 100 units mL⁻¹ penicillin and 100 mg mL⁻¹ streptomycin sulfate at 37°C in 5% CO₂. CRL-2843-CD163 cells were maintained in Roswell Park Memorial Institute 1640 medium (Solarbio, Cat. No. 31800) supplemented with 10% FBS and 100 units mL⁻¹ penicillin and 100 mg mL⁻¹ streptomycin sulfate at 37°C in 5% CO₂.
HP-PRRSV strain HN07-1 (GenBank: KX766378.1) and NADC30-like PRRSV strain HNhx (GenBank: KX766379) were previously isolated by our laboratory (72 (link), 73 (link)). Low-pathogenic PRRSV strain BJ-4 (GenBank: AF331831) was kindly provided by Professor Hanchun Yang of China Agricultural University. The HP-PRRSV strain HN07-1 was utilized in the current study unless otherwise stated.

Zhao S.S., Qian Q., Chen X.X., Lu Q., Xing G., Qiao S., Li R, & Zhang G. (2024). Porcine reproductive and respiratory syndrome virus triggers Golgi apparatus fragmentation-mediated autophagy to facilitate viral self-replication. Journal of Virology, 98(2), e01842-23.

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Viral Infection Assays in Cell Lines

Cited 2 times

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PK-15, Vero, MARC-145, and HeLa cells were grown in monolayers at 37 °C under 5% CO₂. All cells were cultured in DMEM (10566–016, GIBCO), supplemented with 10% FBS (10099141C, GIBCO), 100 units/mL penicillin, and 100 μg/mL streptomycin sulfate (B540732, Sangon).
PRV-GFP and PRV-QXX were used as previously described [31 (link),32 (link)]. PRV HN1201 [33 ] was a gift from Professor Ke-Gong Tian (Henan Agricultural University, China). Vesicular stomatitis virus (VSV)-GFP, Newcastle disease virus (NDV)-GFP, influenza virus (H1N1 PR8), and Sendai virus (SeV) [34 (link)] were gifts from Yong-Tao Li (Henan Agricultural University, China). Porcine reproductive and respiratory syndrome virus (PRRSV)-GFP [35 (link)] was a gift from Professor En-Min Zhou (Northwest A&F University, China).

Zeng L., Wang M.D., Ming S.L., Li G.L., Yu P.W., Qi Y.L., Jiang D.W., Yang G.Y., Wang J, & Chu B.B. (2020). An effective inactivant based on singlet oxygen-mediated lipid oxidation implicates a new paradigm for broad-spectrum antivirals. Redox Biology, 36, 101601.

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Isolation and Culture of Porcine Alveolar Macrophages

Cited 1 time

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PAMs were collected from lung lavage samples obtained from 4-week-old specific-pathogen-free piglets (free of PRRSV-1, PRRSV-2, pseudorabies virus, porcine circovirus, and classical swine fever virus). PAMs were cultured in RPMI 1640 (GIBCO, 61870036) supplemented with 10% fetal bovine serum (FBS) (GIBCO, 10099141C), 100 units/mL penicillin, and 100 μg/mL streptomycin sulfate (Sangon, B540732). MARC-145 cells (American Type Culture Collection, ATCC, CRL-12231) and HEK293T cells (ATCC, CRL-11268) were cultivated as previously described [47 (link)]. All cells were grown in monolayer cultures at 37°C under 5% CO₂.

Li G.L., Han Y.Q., Su B.Q., Yu H.S., Zhang S., Yang G.Y., Wang J., Liu F., Ming S.L, & Chu B.B. (2024). Porcine reproductive and respiratory syndrome virus 2 hijacks CMA-mediated lipolysis through upregulation of small GTPase RAB18. PLOS Pathogens, 20(4), e1012123.

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Culturing Human Lung and Kidney Cells

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Human lung cancer cell lines A549, A549/DDP, and human embryonic kidney cells HEK-293T were purchased from Shanghai Institute of Biosciences Cell Resource Center (Shanghai, China) and cultured in RPMI-1640 (Gibco Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS; Biological Industries Israel Bei-Haemek Ltd., Israel), 100 U/mL of penicillin sodium, and 100 μg/mL of streptomycin sulfate (Sangon Biotech Shanghai, China) at 37 °C in a humidi ed atmosphere of 5% CO2 (Thermo Electron, USA) [26] .

Shao S., Du Y., Zhu S., Zhang W., Zhang Z., Sun Y., Cui T., Liu X., Feng Y., & Liu C. (2020). Effect of EGR1-Mediated LncRNA HOTAIR on MRP1 Gene Expression and MDR in Lung Cancer.

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