Unless otherwise mentioned all chemicals used are of the highest quality available and purchased from Sigma Aldrich (St. Louis, MO, USA).
agarose,
agar,
ethidium bromide,
catalase,
methanol,
ethanol,
isopropanol,
DMSO,
NaCl,
EDTA, Tris-HCl,
Triton-X 100,
NaOH,
paraformaldehyde, bovien serum albumin (BSA), biotin,
histidine,
ampicillin, oxoid nutrient broth No. 2, and
protease inhibitor cocktail were obtained from HiMedia (Mumbai, India), sodium fluoride and orthovandate from MP Biomedicals, LLC (Solon Ohio, USA), CellROX Deep Red and Nuc Blue (Hoechst 33342) from ThermoFisher Scientific (Santa Clara, CA),
Salmonella typhimurium TA100 from Microbial Type Culture Collection and Gene Bank (MTCC, Chandigarh, India), thalidomide and DMEDA from Tokyo Chemical Industry (Tokyo, Japan), nitrocellulose membranes from MDI Membrane Technologies (Ambala. India), LMPA and LE
agarose from Lonza (Rockland, ME, USA),
γ-H2AX Alexa Fluor 488 from Biolegend (San Diego, CA, USA), and PAD-PARP antibody from Abcam (MA, USA). The 293T cell line was purchased from National Centre for Cell Culture (Pune, India) and authenticated by Short Tandem Repeat DNA profiling from Life Code Technology, Delhi, India. HepG2 cells were a generous gift from Dr. Soumya Sinha Roy at the CSIR Institute of Genomics and Integrative Biology, New Delhi.
HUVEC was procured from HiMedia (Mumbai, India).
Wani T.H., Chakrabarty A., Shibata N., Yamazaki H., Guengerich F.P, & Chowdhury G. (2017). The Dihydroxy Metabolite of the Teratogen Thalidomide Causes Oxidative DNA Damage. Chemical research in toxicology, 30(8), 1622-1628.
