Nhek cell line

Cell cultures were established as described by Kordulewska et al 2021.29 (link) In brief, the Normal Human Epidermal Keratinocyte (NHEK) cell line was purchased from PromoCell GmbH (Heidelberg, Germany, cat. no. C-12005) and cultured in T-75 flasks in keratinocyte medium (Keratinocyte Growth Medium 2 ready to use, PromoCell, Heidelberg, Germany, cat. No. C-20011). Media and supplements were mixed according to the manufacturer’s instructions. NHEK cells were incubated at 37°C in a 95% humidified atmosphere with 5% CO2. The culture medium was changed every 2–3 days. NHEK cells were passaged when confluence reached approximately 80%. Only the early passages (3–7) were used for further analysis.
Normal Human Dermal Fibroblasts (NHDF; from juvenile foreskin) were purchased from PromoCell GmbH (Heidelberg, Germany, cat. no. C-12300) and cultured in a T-75 flask in recommended culture medium (PromoCell GmbH, Heidelberg, Germany, cat. no. C-23010). The medium and supplements were mixed according to the manufacturer’s instructions and contained 1% penicillin/streptomycin (Sigma Aldrich, St. Louis, MO, USA, cat. no. P4333). The NHDF cell line was incubated at 37°C in a 95% humidified atmosphere and 5% CO2. The culture medium was changed every 2–3 days and the cells were passaged when confluence reached approximately 80–90%.29 (link)

The NHEK cell line was purchased from PromoCell GmbH (Heidelberg, Germany, cat. no. C-12005) and cultured in T-75 flasks in keratinocyte medium (Keratinocyte Growth Medium 2 ready to use, PromoCell, Heidelberg, Germany, cat. no. C-20011) prepared according to the manufacturer’s instructions. NHEKs were incubated at 37°C in a 95% humidified atmosphere and 5% CO₂. The culture medium was changed every 2–3 days, and cells were passaged when confluence reached approximately 80%. Only early passages (3–7) were used in this study.
The NHDF cell line (PromoCell GmbH, Heidelberg, Germany, cat. no. C-12300) was cultured in a T-75 flask in the recommended culture medium (PromoCell GmbH, Heidelberg, Germany, cat. no. C-23010) containing 1% of penicillin/streptomycin solution (Sigma Aldrich, St. Louis, MO, USA, cat. no. P4333). Cells were incubated at 37°C in a 95% humidified atmosphere and 5% CO2. The culture medium was changed every 2–3 days, and cells were passaged when confluence reached approximately 80%.