Sc 1052

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Sc-1052 is a laboratory equipment product offered by Santa Cruz Biotechnology. The core function of this product is to facilitate specific laboratory procedures, but a detailed description cannot be provided while maintaining an unbiased and factual approach.

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Lab products found in correlation

6 protocols using sc 1052

Antibody Screening and Cell Death Analysis

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Goat antibodies against Hsp90 (SC-1055), Hsp70 (SC-1060), Hsp60 (SC-1052), Hsp40 (SC-1801), Hsc70 (SC-1059), integrin β3 (SC-6626), PDI (SC-17222), Cox-2 (SC-1747), and cleaved PARP-1 (SC-56196) were obtained from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA). Rabbit antibodies against NF-kB p65 (phospho S536) (ab86299) were purchased from Cambridge Science Park (Cambridge, UK). Donkey anti-goat Alexa Fluor 594-conjugated secondary antibodies (SC- 362275) and donkey anti- rabbit-Alexa Fluor 594- conjugated secondary antibodies (SC-362281) were obtained from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA). Donkey anti-goat or anti-rabbit antibodies conjugated with FITC (SC-362255 and SC-362261, respectively) or HRP (SC-2020 and SC-2313, respectively) were also obtained from Santa Cruz Biotechnology Inc. 7-aminoactinomycin D (7-AAD), propidium iodide, and 4,’6-diamidino-2-phenylindole (DAPI) were purchased from Invitrogen (Carlsbad, CA, USA). Annexin V-Alexa 568 kit, Apoptotic DNA- Ladder Kit, In Situ Cell Death Detection kit, and poly(ADP-ribose) polymerase (PARP) were obtained from Roche Laboratories, Inc. (Nutley, NJ, USA), and Hoechst 33342 from Thermo Scientific (Waltham, MA, USA).

Guerrero R.A., Guerrero C.A., Guzmán F, & Acosta O. (2020). Assessing the oncolytic potential of rotavirus on mouse myeloma cell line Sp2/0-Ag14. Biomédica, 40(2), 362-381.

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OXPHOS Protein Detection Protocol

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Cells were washed with 1mL cold 1x PBS and scraped in 40µL
bio-plex cell lysis buffer (Biorad), and processed according to the
manufacturers guidelines. OXPHOS proteins were detected using a cocktail of five
monoclonal antibodies directed against structural components of the different
OXPHOS complexes (MS601, MitoSciences, Eugene, OR), as previously described
(Hoeks et al., 2010 (link)). Furthermore,
VDAC (sc-8828, Santa Cruz Biotech, Heidelberg, Germany), HSP60 (Sc-1052 Santa
Cruz Biotech, Heidelberg, Germany) and HSP90 (610418, BD Biosciences, Breda,
Netherlands) were detected using separate antibodies from different
manufacturers.

Dahlmans D., Houzelle A., Andreux P., Wang X., Jörgensen J.A., Moullan N., Daemen S., Kersten S., Auwerx J, & Hoeks J. (2018). MicroRNA‐382 silencing induces a mitonuclear protein imbalance and activates the mitochondrial unfolded protein response in muscle cells. Journal of Cellular Physiology, 234(5), 6601-6610.

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Immunofluorescence Characterization of Neurons

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mouse anti-β-III-tubulin (Sigma #T8660; 1:2000), mouse anti-microtubule-associated protein 2a+b (MAP2, Sigma #M1406; 1:2000), rabbit anti-tyrosine hydroxylase (TH, Millipore #AB152; 1:600), mouse anti-synaptophysin (Sigma #S5768; 1:200), goat anti-forkhead box A2 (FOXA2 (Sigma #AF2400; 1:250), rabbit anti-GABA (Sigma #A2052; 1:2000), rabbit anti-GFAP (DAKO #Z0334; 1:4000), rabbit anti-TOM20 (Santa Cruz #SC-11415, 1:1000), goat anti-HSP60 (Santa Cruz Biotechnology #SC-1052, 1:200).

Okarmus J., Agergaard J.B., Stummann T.C., Haukedal H., Ambjørn M., Freude K.K., Fog K, & Meyer M. (2024). USP30 inhibition induces mitophagy and reduces oxidative stress in parkin-deficient human neurons. Cell Death & Disease, 15(1), 52.

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Mitochondrial Dynamics Protein Antibodies

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Antibodies to the following proteins were used: HSP60 (goat; sc-1052; Santa Cruz Biotechnology, Inc.), ubiquitin (mouse; 3936P; Cell Signaling Technology), actin (rabbit; A2103; Sigma-Aldrich), histone H2B (rabbit; IMG-359; Imgenex), Mfn1 (raised in chicken against mouse Mfn1 residues 348–579; Chen et al., 2003 (link)), Mfn2 (rabbit; D2D10; Cell Signaling Technology), Opa1 (mouse ascites produced in-house against the mouse S1 isoform), Fis1 (rabbit; ALX-210-1037-0100; Enzo Life Sciences), Drp1 (mouse; 611113; BD), and Mff (raised in rabbit against human Mff by A. van der Bliek, UCLA, Los Angeles, CA; Gandre-Babbe and van der Bliek, 2008 (link)). All secondary antibodies for Western blots were HRP conjugated and obtained from Jackson ImmunoResearch Laboratories, Inc.

Chen H., Ren S., Clish C., Jain M., Mootha V., McCaffery J.M, & Chan D.C. (2015). Titration of mitochondrial fusion rescues Mff-deficient cardiomyopathy. The Journal of Cell Biology, 211(4), 795-805.

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Immunocytochemistry of HepG2 cells

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HepG2 cells were grown following the standard ENCODE protocol (DMEM media, 4 mM L-glutamine, 4.5 g/L glucose, without sodium pyruvate, with 10% FBS (Invitrogen 10091-148) and penicillin-streptomycin). Cells were fixed in 10% formalin (Sigma-Aldrich HT501128-4L) for 10 min, permeabilized with 0.1% Triton X-100, and blocked in 5% FBS. Primary antibodies used were MafK (1∶100, Abcam, ab50322) and Hsp60 (1∶125, Santa Cruz, sc-1052). Secondary antibodies used were donkey anti-goat AF488 (Invitrogen A11055) and donkey anti-rabbit AF546 (Invitrogen A10040). Imaging on a Zeiss LSM 710 confocal microscope with PlanApochromat 63X/1.4 oil objective, and 0.7 µm optical sections were acquired.

Marinov G.K., Wang Y.E., Chan D, & Wold B.J. (2014). Evidence for Site-Specific Occupancy of the Mitochondrial Genome by Nuclear Transcription Factors. PLoS ONE, 9(1), e84713.

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Protein Expression Analysis by Western Blot

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Forty microgram proteins extracted from the liver was separated in 8–15%SDS-PAGE, then transferred onto PVDF membrane and incubated with primary antibodies at 4 °C overnight. The primary antibodies included PI3Kp85α (1:5000; 60225-1 lg, PROTEINTECH, IL, USA), CHOP (1:1000; #2895, cell signaling, MA, USA), SQSTM1/p62 (1:5000; GTX111393, GeneTex, CA, USA), HSP60 (1:5000; sc-1052, Santa Cruz, CA, USA), LONP1 (1:2000; 15440-1-AP, Proteintech, IL, USA), LC3B II (1:5000; #2775, cell signaling, MA, USA), and α-SMA (1:1000; ab5694, abcam, Cambridge, UK). GAPDH (1:100,000; 60004-1 lg, PROTEINTECH, IL, USA) was used for probing protein loading control. After washing twice with TBST solution, PVDF membrane was incubated with secondary antibodies such as horseradish peroxidase-coupled antirabbit immunoglobulin-G antibodies (1:5000; NEF812001EA, PerkinElmer, MA, USA) or HRP antimouse immunoglobulin-G antibodies (1:10,000; NEF822001, PerkinElmer) at room temperature for 1 h. The blots were developed with an ECL Western blotting detection and analysis system (Amersham Pharmacia Biotech, Uppsala, Sweden) and exposed them to film. The signals were quantified by using Quantity One^® 1-D analysis software (Bio-Rad Laboratories).

Yang Y.L., Wang F.S., Lin H.Y, & Huang Y.H. (2020). Exogenous Therapeutics of Microrna-29a Attenuates Development of Hepatic Fibrosis in Cholestatic Animal Model through Regulation of Phosphoinositide 3-Kinase p85 Alpha. International Journal of Molecular Sciences, 21(10), 3636.

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