All plasmid vectors for transfection were extracted by DNA Midiprep kit (Qiagen, Hilden, Germany). Three individual SNHG16 siRNAs (si-SNHG16) and scrambled negative control siRNA (si-NC) were purchased from Ribo BioCoLTD (Guangzhou, China). The target sequences of siRNA were as follows: si-NC, 5′-UUCUCCGAACGUGUCACGUTT-3′; si-SNHG16 1, 5′-GGAAUGAAGCAACUGAGAUUU-3′; si-SNHG16 2, 5′-CATGTCCTTCTGATCACCAAGTTGACTTA-3′; si-SNHG16 3, 5′-GATATCTTAGTCCTAACCATATTGATCCC-3′. Lipofectamine™ 2000 transfection reagent (Invitrogen, U.S.A.) was used to transfected oligonucleotide and plasmid, followed by the manufacturer’s protocol. After transfection for 48 h, cells were further used in the relevant experiments. Plasmid complementary DNA SNHG16 cDNA (pcDNA-SNHG16) was constructed by amplification and introduction of SNHG16 cDNA sequence into the pcDNA3.1 vector (Invitrogen).
Si snhg16
Manufactured by RiboBio
Sourced in China
Si-SNHG16 is a small interfering RNA (siRNA) that targets the SNHG16 gene. SNHG16 is a long non-coding RNA that has been implicated in various cellular processes. The Si-SNHG16 siRNA is designed to downregulate the expression of SNHG16 in cells.
Automatically generated - may contain errors
Lab products found in correlation
Si nc, by RiboBio (3 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (3 mentions)
Pcdna snhg16, by RiboBio (2 mentions)
Mir nc, by RiboBio (2 mentions)
Si nc, by Thermo Fisher Scientific (1 mentions)
Dna midiprep kit, by Qiagen (1 mentions)
Lipofectamine 2000 transfection reagent, by Thermo Fisher Scientific (1 mentions)
Pcdna3.1 vector, by Thermo Fisher Scientific (1 mentions)
Anti nc, by RiboBio (1 mentions)
Sirna scrambled control si nc, by RiboBio (1 mentions)
Pcdna vector, by RiboBio (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Inhibitor nc, by RiboBio (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
Sw480, by American Type Culture Collection (1 mentions)
Sh nc, by Genechem (1 mentions)
Ccd 841 con, by American Type Culture Collection (1 mentions)
Lipofectamine 2000 reagent, by Thermo Fisher Scientific (1 mentions)
5 protocols using si snhg16
1
Plasmid Transfection and SNHG16 Silencing
2
Investigating SNHG16 Regulation in Glioma Cells
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SNHG16-overexpression vector (SNHG16) and its negative control (Vector), specific small interfering RNA (siRNA) against SNHG16 (si-SNHG16) and siRNA scrambled control (si-NC), miR-424-5p mimic and miR-NC, Anti-miR-424-5p, and Anti-NC were purchased from RiboBio (Guangzhou, China). Construction of SNHG16-overexpression vector (SNHG16) was accomplished by amplifying cDNA of SNHG16 and subcloning into the pcDNA vector (RiboBio). The vectors or oligonucleotides were transfected into T98G or LN229 cells by Lipofectamine 2000 (Thermo Fisher Scientific) in compliance with the manufacturer’s protocol. The primers for SNHG16-overexpression were F 5′-aagcttGCGTTCTTTCGAGG-3′ and R 5′-ggatccTGACGGTAGTTTCCC-3′, including HindIII and BamHI restriction sites. The sequence for si-SNHG16 was 5′-UAAAGACAUGGCACUUUGGGU-3′, and the sequence for si-NC was 5′-UUCUCCGAACGUGUCACGUTT-3′.
3
Colorectal Cancer Cell Line Manipulation
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CRC cell lines (SW480 and SW620) and normal human colonic epithelial cells (CCD841 CON) were bought from the American Type Culture Collection (ATCC, Manassas, VA, USA). Culture medium was contained with RPMI-1640 (Gibco, Waltham, MA, USA), 10% fetal bovine serum (FBS; Gibco), 100 U/mL penicillin and 0.1 mg/mL streptomycin (Invitrogen, Carlsbad, CA, USA), and all cells were cultured in a 5% CO2 at 37°C incubator. Small interfering RNA (siRNA) against SNHG16 and ubiquitin specific peptidase 22 (USP22) (si-SNHG16 and si-USP22) or negative control (si-NC), SNHG16 and USP22 overexpression plasmids (pcDNA-SNHG16 and pcDNA-USP22) or negative control (pcDNA), miR-132-3p mimic (miR-132-3p), miR-132-3p inhibitor and their negative controls (miR-NC and inhibitor-NC) were purchased from RiboBio (Guangzhou, China). Lentiviral short hairpin RNA (shRNA) targeting SNHG16 (sh-SNHG16) and negative control (sh-NC) were synthesized by Genechem (Shanghai, China). All oligonucleotides or plasmid vectors were transfected into SW480 and SW620 cells using Lipofectamine 3000 (Invitrogen).
4
Regulation of SNHG16 and HNF4α in Neuroblastoma
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Small interfering RNA (siRNA) against SNHG16 (si-SNHG16) and HNF4α (si-HNF4α), siRNA negative control (si-NC), short hairpin RNA (shRNA) against SNHG16 (sh-SNHG16), shRNA negative control (sh-NC), overexpression vectors of SNHG16 (pcDNA-SNHG16) and HNF4α (pcDNA-HNF4α), as well as their control constructed by pcDNA3.1 vector (pcDNA) were designed and synthesized in Ribobio (Shanghai, China). Furthermore, miR-542-3p mimic (miR-542-3p) and its blank control (miR-NC) were constructed from Ribobio. All the sequences were introduced into SKNBE-2 and SK-N-SH cells using Lipofectamine 2000 reagent (Invitrogen) based on the specifications. Meanwhile, lentivirus-mediated sh-SNHG16 or sh-NC was introduced into SKNBE-2 cells to form stably transfected cells.
5
Transfection of miR-628 and SNHG16 modulators
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MiR-628 agomir (agomir-628), negative control (NC) agomir (agomir-NC), miR-628 antagomir (antagomir-628), and NC antagomir (antagomir-NC) were purchased from Shanghai GenePharma Co., Ltd. (Shanghai, China). The siRNA that was used to silence SNHG16 (si-SNHG16) and negative control siRNA (si-NC) were generated by Guangzhou RiboBio Co., Ltd. (Guangzhou, China). The plasmid expressing NRP1 (pcDNA3.1-NRP1) was constructed by GenScript Biotech Corp. (Nanjing, China). Cells were seeded in 6-well plates 24 h before transfection. The above-mentioned oligonucleotides and plasmid were transfected into cells by means of Lipofectamine 2000 (Invitrogen; Thermo Fisher Scientific, Inc.).
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