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Anti visfatin

Manufactured by Santa Cruz Biotechnology
Sourced in United States

Anti-Visfatin is a laboratory reagent produced by Santa Cruz Biotechnology. It is an antibody that specifically binds to the visfatin protein, a multifunctional enzyme involved in various cellular processes. The core function of Anti-Visfatin is to enable the detection and quantification of visfatin in biological samples using techniques such as Western blotting, ELISA, and immunohistochemistry.

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2 protocols using anti visfatin

1

Comprehensive Biomarker Immunoassay Panel

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Anti-APP, anti-Aβ1–42 monomer, anti-Aβ1–42 oligomer, anti-Tau, anti-STAT3, anti-NLRP3, anti-ApoE4, anti-Nogo-A, anti-IL-13, anti-TNFα, anti-GFAP, anti-SORL1, anti- UCHL1, anti-Visfatin and anti-Clusterin were used (Santa Cruz, USA).
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2

Protein Expression Analysis of Arterial Wall

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Snap-frozen arterial wall or PVAT were pooled (n = 4 each group)
for the preparation of whole cell extracts in ice-cold lysis buffer and electrophoretically separated on 10% acrylamide/Bis-acrylamide gels. Blots were incubated at room temperature with blocking solution (4% nonfat dry milk in PBS containing 0.1% Tween-20) for 30-40 minutes, followed by 1 hour incubation with the following primary antibodies: Anti visfatin (Santa Cruz Biotechnology 1/200), anti MMP-9 (Sigma Aldrich, St. Louis, MO, USA, 1/100); anti actin (Santa Cruz Biotechnology 1/200, sc-3035), and anti tubulin (Santa Cruz Biotechnology 1/200, sc-8035).
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