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NUGC4 is a commercial cell line product offered by the Japanese Collection of Research Bioresources Cell Bank. It is derived from a human gastric carcinoma. The core function of NUGC4 is to serve as a research tool for studies involving human gastric cancer cells.

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Lab products found in correlation

Mkn45, by Japanese Collection of Research Bioresources Cell Bank (13 mentions) Mkn74, by Japanese Collection of Research Bioresources Cell Bank (10 mentions) Kato 3, by Japanese Collection of Research Bioresources Cell Bank (9 mentions) Nugc3, by Japanese Collection of Research Bioresources Cell Bank (8 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (5 mentions) Nugc2, by Japanese Collection of Research Bioresources Cell Bank (4 mentions) Ocum 1, by Japanese Collection of Research Bioresources Cell Bank (4 mentions) Kato 3, by American Type Culture Collection (4 mentions) Sc 6 jck, by Japanese Collection of Research Bioresources Cell Bank (3 mentions) Powerplex hs16 system kit, by Promega (3 mentions) Mycoalert plus mycoplasma detection kit, by Lonza (3 mentions) Dulbecco modified eagle medium (dmem), by Fujifilm (2 mentions) E myco mycoplasma pcr detection kit, by Cosmo Bio (2 mentions) L glutamine, by Thermo Fisher Scientific (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions) Rpmi medium, by Thermo Fisher Scientific (1 mentions) Fhs74, by American Type Culture Collection (1 mentions) Mkn45, by National Infrastructure of Cell Line Resource (1 mentions) Hek293t cell, by American Type Culture Collection (1 mentions)

22 protocols using nugc4

1

Gastric Cancer Cell Line Characterization

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We used GC cell lines, which were obtained from the Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan) as follows: MKN1, MKN45, MKN74, NUGC2, NUGC3, NUGC4, and SC-6-JCK. The GC cell lines AGS, KATOIII, and N87 were purchased from the American Type Culture Collection (ATCC) (Manassas, VA, USA). The human intestinal epithelial cell line FHs74Int (ATCC) served as a nontumorigenic control. Primary GC tissues and corresponding noncancerous mucosal tissues were collected from 200 patients who underwent gastrectomy at Nagoya University Hospital between 2001 and 2014. None of the patients underwent preoperative chemotherapy.
The methods were carried out in accordance with relevant guidelines. The study protocol was approved by the Medical Ethics Committee of the Nagoya University Hospital, protocol No. 2014–0043. Informed consent was obtained from all patients. Written informed consent for the use of clinical samples and data, as required by the Institutional Review Board, was obtained from all patients.
Shimizu D., Kanda M., Tanaka H., Kobayashi D., Tanaka C., Hayashi M., Iwata N., Niwa Y., Takami H., Yamada S., Fujii T., Nakayama G., Fujiwara M, & Kodera Y. (2017). GPR155 Serves as a Predictive Biomarker for Hematogenous Metastasis in Patients with Gastric Cancer. Scientific Reports, 7, 42089.
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2

Gastric Cancer Cell Line Characterization

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GC cell lines, the differentiated type (AGS, IM95, MKN1, MKN7, MKN74 and N87) and the undifferentiated type (GCIY, KATO-III, MKN45, NUGC2, NUGC3, NUGC4, OCUM1 and SC-6-JCK), were obtained from the Japanese Collection of Research Bio Resources Cell Bank (Osaka, Japan) or the American Type Culture Collection (ATCC, Manassas, VA, USA). A control, non-tumorigenic epithelial cell line (FHs 74) was purchased from the ATCC. The cells were cultured at 37 °C in RPMI medium (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% foetal bovine serum in an atmosphere containing 5% CO2. All cell lines were authenticated using the short tandem repeat PCR method by the Japanese Collection of Research Bio Resources Cell Bank before the study commenced.
Miwa T., Kanda M., Shimizu D., Umeda S., Sawaki K., Tanaka H., Tanaka C., Hattori N., Hayashi M., Yamada S., Nakayama G., Koike M, & Kodera Y. (2021). Hepatic metastasis of gastric cancer is associated with enhanced expression of ethanolamine kinase 2 via the p53–Bcl-2 intrinsic apoptosis pathway. British Journal of Cancer, 124(8), 1449-1460.
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3

Cultivation of Human Gastric Cancer Cell Lines

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Six human gastric cancer cell lines (MKN7, MNK74, MNK45, NUGC3, NUGC4, and KATOIII) were obtained from the JCRB Cell Bank (Osaka, Japan) and the RIKEN Bioresource Center (Tsukuba, Japan). All cell lines were cultured in RPMI 1640 supplemented with 10% fetal bovine serum at 37 °C in a humidified atmosphere containing 5% CO2.
Soda T., Gen Y., Terasaki K., Iwai N., Kitaichi T., Dohi O., Taketani H., Seko Y., Umemura A., Nishikawa T., Yamaguchi K., Moriguchi M., Konishi H., Naito Y., Itoh Y, & Yasui K. (2022). Loss of KAP3 decreases intercellular adhesion and impairs intracellular transport of laminin in signet ring cell carcinoma of the stomach. Scientific Reports, 12, 5050.
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4

Gastric Cancer Cell Lines and Tissues

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The GC cell lines GCIY, IM95, MKN1, MKN7, MKN45, MKN74, NUGC2, NUGC3, NUGC4, OCUM-1, and SC-6-JCK were obtained from the Japanese Collection of Research Bioresources Cell Bank (JCRB, Osaka, Japan). AGS, KATOIII, and N87 GC cell lines and a nontumorigenic epithelial cell line (FHs74) were acquired from the American Type Culture Collection (Manassas, VA, USA). Three hundred pairs of surgically resected GC and adjacent noncancerous tissues were obtained from patients who underwent gastrectomy. A freely available integrated dataset (n = 1065 GC patients) was accessed at http://kmplot.com/analysis/ [13 (link)].
Kanda M., Shimizu D., Sawaki K., Nakamura S., Umeda S., Miwa T., Tanaka H., Tanaka C., Hayashi M., Iguchi Y., Yamada S., Katsuno M, & Kodera Y. (2020). Therapeutic monoclonal antibody targeting of neuronal pentraxin receptor to control metastasis in gastric cancer. Molecular Cancer, 19, 131.
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5

Culturing Diffuse-Type GC Cell Lines

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The diffuse type GC cell lines MKN45 (3111C0001CCC000229) was obtained from the National Infrastructure of Cell Line Resource (Beijing, China), and NUGC4 (JCRB0834) was from JCRB cell bank (Osaka, Japan). The human peritoneal mesothelial cell line HMR-SV5 was gifted from Prof. Huimian Xu (Department of Surgical Oncology and General Surgery, The First Hospital of China Medical University). All the cells were cultured in RPMI-1640 medium containing 10% heat-inactivated FBS at 37°C under 5% CO2 and saturated humidity.
Bao B., Zheng C., Yang B., Jin Y., Hou K., Li Z., Zheng X., Yu S., Zhang X., Fan Y., Qu X., Liu Y, & Che X. (2019). Identification of Subtype-Specific Three-Gene Signature for Prognostic Prediction in Diffuse Type Gastric Cancer. Frontiers in Oncology, 9, 1243.
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6

Culturing HEK293T and 3T3 Fibroblasts

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Human embryonic kidney (HEK) 293T cells and mouse 3T3 fibroblasts were obtained from the ATCC (https://www.atcc.org). SGC cell lines, KATO‐III and NUGC4, were purchased from Japanese Collection of Research Bioresources (http://cellbank.nibiohn.go.jp/english/). OCUM‐1, ‐2M, ‐8, ‐9 and ‐12 were established by M.Y. All cell lines were maintained in DMEM‐F12 medium supplemented with 10% FBS and 2 mmol/L glutamine (all from Invitrogen).
Sai E., Miwa Y., Takeyama R., Kojima S., Ueno T., Yashiro M., Seto Y, & Mano H. (2019). Identification of candidates for driver oncogenes in scirrhous‐type gastric cancer cell lines. Cancer Science, 110(8), 2643-2651.
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7

Gastric Cancer Cell Line Characterization

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Human GC cell lines MKN1, NUGC4 (Japanese Collection of Research Bioresources Cell Bank) and AGS (American Type Culture Collection, ATCC) were cultured in Roswell Park Memorial Institute 1640 (RPMI 1640) medium supplemented with 10% fetal calf serum (FCS) and 1% L‐glutamine (Gibco). Cells were authenticated by short tandem repeat profiling (PowerPlex HS16 System kit, Promega), and were passaged for under 6 months after receipt. Cells were routinely tested for mycoplasma contamination (MycoAlert PLUS Mycoplasma Detection Kit, Lonza). AZ521, a duodenal adenocarcinoma line was characterized a Hutu 80 derivative. DNA array data generated from this line was only used as a TLR2 low expressing cell comparison for gene profiling and signaling pathway analysis. No experiment on AZ521 was conducted in our study.
Liu Y.D., Yu L., Ying L., Balic J., Gao H., Deng N.T., West A., Yan F., Ji C.B., Gough D., Tan P., Jenkins B.J, & Li J.K. (2019). Toll‐like receptor 2 regulates metabolic reprogramming in gastric cancer via superoxide dismutase 2. International Journal of Cancer, 144(12), 3056-3069.
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8

Hypoxia-induced metabolic reprogramming

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GC cell lines MKN45, NUGC-4, and MKN74 were purchased from the Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan). The cells were exposed to hypoxia (0.1% O2) using a BIONIX system (Sugiyama-gen Co.). Palmitic acid (PA; P9767, Sigma Aldrich, UK) was prepared as 2 mM stock solution by dissolving in 75% ethanol at 70 °C. Stock solutions were then added to 2% FA-free bovine serum albumin (BSA; 017-15146, FUJIFILM, Japan) to achieve the desired final FA concentration. PA or etomoxir (10 mM in dimethyl sulfoxide [DMSO], 124083-20-1, ChemScene, Japan) was added to the medium, and 75% ethanol and DMSO were used as respective controls.
Aoki T., Kinoshita J., Munesue S., Hamabe-Horiike T., Yamaguchi T., Nakamura Y., Okamoto K., Moriyama H., Nakamura K., Harada S., Yamamoto Y., Inaki N, & Fushida S. (2022). Hypoxia-Induced CD36 Expression in Gastric Cancer Cells Promotes Peritoneal Metastasis via Fatty Acid Uptake. Annals of Surgical Oncology, 30(5), 3125-3136.
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9

Culturing Cancer and Control Cell Lines

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The human OSCC cell line (HSC-3) and the human gastric cancer cell lines (MKN45 and NUGC-4) were obtained from the Japanese Collection of Research Bioresources (Osaka, Japan). The human pancreatic cancer cell line (PANC-1) was obtained from the Cell Resource Center for Biomedical Research Institute of Development, Aging, and Cancer at Tohoku University (Sendai, Japan). Chinese hamster ovary (CHO)-K1 and P3X63Ag8U.1 (P3U1; a mouse multiple myeloma) cell lines were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). HSC-3 was cultured in DMEM medium (Nacalai Tesque, Inc., Kyoto, Japan), supplemented with 100 μg/mL streptomycin, 100 U/mL penicillin, and 0.25 μg/mL amphotericin B (Nacalai Tesque, Inc.), and 10% (v/v) heat-inactivated fetal bovine serum (FBS; Thermo Fisher Scientific, Inc., Waltham, MA, USA). The cell lines (MKN45, NUGC-4, PANC-1, CHO-K1, and P3U1) were cultured in RPMI-1640 medium (Nacalai Tesque, Inc.), supplemented as indicated above. All cells were cultured using a humidified incubator at 37 °C, in an atmosphere of 5% CO2 and 95% air.
Suzuki H., Goto N., Tanaka T., Ouchida T., Kaneko M.K, & Kato Y. (2023). Development of a Novel Anti-CD44 Variant 8 Monoclonal Antibody C44Mab-94 against Gastric Carcinomas. Antibodies, 12(3), 45.
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10

Gastric Cancer Cell Culture

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Human GC cell lines, OCUM‐2MD3,30 KATO‐III, MKN45, and NUGC4 (JCRB Cell Bank) were used. The cells were cultured with DMEM (Wako) at 37°C in 21% O2 with 5% CO2.
Kuroda K., Yashiro M., Miki Y., Sera T., Yamamoto Y., Sugimoto A., Nishimura S., Kushiyama S., Togano S., Okuno T, & Ohira M. (2020). Circulating tumor cells with FGFR2 expression might be useful to identify patients with existing FGFR2‐overexpressing tumor. Cancer Science, 111(12), 4500-4509.
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