Log In Sign up
The largest database of trusted experimental protocols

α-Myc is a laboratory reagent used for the detection and analysis of the c-Myc protein, a transcription factor involved in various cellular processes. It functions as a primary antibody that specifically binds to the c-Myc protein, enabling its identification and quantification in various experimental techniques.

Automatically generated - may contain errors

Lab products found in correlation

α gfp, by Santa Cruz Biotechnology (3 mentions) α flag, by Merck Group (3 mentions) N dodecyl n n dimethylamine n oxide, by Anatrace (2 mentions) 1 palmitoyl 2 oleoyl sn glycerol 3 phosphocholine, by Avanti Polar Lipids (2 mentions) α tubulin, by Merck Group (2 mentions) Restriction endonuclease, by New England Biolabs (1 mentions) Anti ha antibody, by Fortrea (1 mentions) Anti v5, by Bio-Rad (1 mentions) α gapdh, by Cell Signaling Technology (1 mentions) Deltavision personaldv microscope, by Cytiva (1 mentions) Softworx software version 5, by Cytiva (1 mentions) α tubulin, by Santa Cruz Biotechnology (1 mentions) Nitrocellulose membrane, by Cytiva (1 mentions) Horseradish peroxidase, by Thermo Fisher Scientific (1 mentions) Whatman, by Cytiva (1 mentions) Protein g plus agarose bead, by Santa Cruz Biotechnology (1 mentions) α pgk1, by Thermo Fisher Scientific (1 mentions) α gfp, by Roche (1 mentions) αgfp antibodies, by Roche (1 mentions) α synaptophysin, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

α-C-MYC (6 citations) α-Myc antibodies (5 citations) α-Myc (9E10, (4 citations) Mouse α-myc (2 citations)

12 protocols using α myc

1

Rac1 and RalBP1 Antibody Sourcing

Check if the same lab product or an alternative is used in the 5 most similar protocols
Monoclonal anti-Rac1 antibody (23A8) was obtained from Millipore. α–human RalBP1 (RLIP76), α–R-Ras, α-Arf6, α-GFP, α-FLAG, α-myc and α-ARNO antibodies were from Santa Cruz Biotechnology, Inc. Anti-HA antibody was from Covance. Restriction endonucleases were from New England Biolabs.
Wurtzel J.G., Lee S., Singhal S.S., Awasthi S., Ginsberg M.H, & Goldfinger L.E. (2015). RLIP76 regulates Arf6-dependent cell spreading and migration by linking ARNO with activated R-Ras at recycling endosomes. Biochemical and biophysical research communications, 467(4), 785-791.
+ Open protocol
+ Expand
2

Antibody Immunoblotting Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Anti-V5, 1:2000, AbD Serotec (MCA1360GA); α-HA, 1:1000, Santa Cruz Biotechnology (F-7: sc-7392); α-FLAG, 1:1000, SIGMA (monoclonal anti-FLAG M2); α-myc, 1:1000, Santa Cruz Biotechnology (9E10, sc-40); α-GFP, 1:1000, Santa Cruz Biotechnology (B-2: sc-9996); α-Pgk1, 1:10000, Santa Cruz Biotechnology (F-7: sc-7392); α-PAP, HRP-conjugated, 1:2000 Sigma (1291); Secondary α-mouse Polyclonal Goat IgG, 1:5000, R&D SYSTEMS (FAH007).
Yahya G., Wu Y., Peplowska K., Röhrl J., Soh Y.M., Bürmann F., Gruber S, & Storchova Z. (2020). Phospho-regulation of the Shugoshin - Condensin interaction at the centromere in budding yeast. PLoS Genetics, 16(8), e1008569.
+ Open protocol
+ Expand
3

Antibody-Based Amyloid-Beta Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used: α-Myc (9E10 Santa Cruz #sc-40), α-Flag (M2 Sigma #F3165), α-Nct (Cell Signaling 3632S), α-GAPDH (Cell Signaling Technology, ab125247), α-APP (C7), α-AICD 50–99 (Rb) was a kind gift from Philip Szekeres at Eli Lilly, α-Ms 800nm (Licor Bio 926–32212) and α-Rb 680nm (Licor Bio 926–68021). Tripeptides were synthesized by Anaspec corp. Total brain lipid extract was from Avanti Polar Lipids (#131101). The following Aβ ELISA kits were used: 4G8 (Meso Scale Diagnostics, K15199E) or 6E10 (Meso Scale Diagnostics, K15200E) for cell-based assays; Aβ40 (#KHB3482) and Aβ42 (#KHB2442) ELISA kits from Invitrogen for in vitro assays.
Bolduc D.M., Montagna D.R., Seghers M.C., Wolfe M.S, & Selkoe D.J. (2016). The amyloid-beta forming tripeptide cleavage mechanism of γ-secretase. eLife, 5, e17578.
+ Open protocol
+ Expand
4

Chromosome Spreads and Immunostaining of Meiotic Proteins in Arabidopsis Pollen Mother Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Chromosome spreads of A. thaliana pollen mother cells and immunostaining of ASY1 and SPO11-1-Myc were performed using fresh buds, as described (Armstrong et al. 2002 (link)). The following antibodies were used: α-ASY1 (rabbit, 1/500 dilution) (Armstrong et al. 2002 (link)) and α-Myc (mouse, 1/50 dilution) (9E10, Santa Cruz Biotechnology). Microscopy was conducted using a DeltaVision Personal DV microscope (Applied Precision/GE Healthcare) equipped with a CDD CoolSNAP HQ2 camera (Photometrics). Image capture and analysis were performed using softWoRx software version 5.5 (Applied precision/GE Healthcare).
Choi K., Zhao X., Tock A.J., Lambing C., Underwood C.J., Hardcastle T.J., Serra H., Kim J., Cho H.S., Kim J., Ziolkowski P.A., Yelina N.E., Hwang I., Martienssen R.A, & Henderson I.R. (2018). Nucleosomes and DNA methylation shape meiotic DSB frequency in Arabidopsis thaliana transposons and gene regulatory regions. Genome Research, 28(4), 532-546.
+ Open protocol
+ Expand
5

Immunoblotting and Immunofluorescence Techniques

Check if the same lab product or an alternative is used in the 5 most similar protocols
For immunoblotting, protein samples were separated on 12% SDS-PAGE and transferred to nitrocellulose membrane (Whatman, NJ). Membranes were incubated with 7% skim milk (in PBST 1X), followed by antibody hybridization. α-Tubulin, α-Myc, α-GFP, α-HA and goat α-RIG-I antibodies were purchased from Santa Cruz Biotech. α-Mitofilin was purchased from NOVUS. α-Flag and α-V5 were purchased from Sigma and Invitrogen respectively. α-IRF3, α-RIG-I and α-FAT10 were purchased from Enzo Life Science, α-phospho IRF3 (Ser369), α-G3BP-1 was purchased from Cell Signaling. α-NP antibody was purchased from Abcam. Immunoreactive signals were detected by enhanced chemiluminescence in horseradish peroxidase (Pierce Biotechnology, MA). For immunofluorescence experiments: Alexa 488-, 568-, 647- conjugated anti-mouse, anti-rabbit, anti-goat IgG antibody purchased from Invitrogen were used as secondary antibodies.
Nguyen N.T., Now H., Kim W.J., Kim N, & Yoo J.Y. (2016). Ubiquitin-like modifier FAT10 attenuates RIG-I mediated antiviral signaling by segregating activated RIG-I from its signaling platform. Scientific Reports, 6, 23377.
+ Open protocol
+ Expand
6

ChIP-qPCR Analysis of Transcription Factors

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Chromatin immunoprecipitation (ChIP) assays were performed according to Blythe et al. (Blythe et al., 2009 (link)). Briefly, stage 10 embryos were fixed with 1% formaldehyde in PBS for 60 minutes at room temperature. After fixation, embryos were homogenized in 600 μl RIPA buffer (50 mM Tris-HCl [pH 7.4], 1 % NP-40, 0.25 % Na-Deoxycholate, 150 mM NaCl, 1 mM EDTA, 0.1 % SDS, 0.5 mM DTT, 5 mM Na-Butyrate, Protease Inhibitor Cocktail, Phosphatase Inhibitor Cocktail), and centrifuged. The pellet was re-homogenized in 650 μl RIPA buffer and crosslinked chromatin is sheared to <1,000-bp fragments by sonication. Sonicated samples were immunoprecipitated by 1 μg α-myc, α-HA, α-HDAC1 (Santa Cruz Biotechnology, Inc) or α-flag (Sigma) antibody with 50 μl Protein G-PLUS agarose beads (Santa Cruz biotechnology, Inc). After immunoprecipitation, eluted DNA was purified by Phenol/Chloroform extraction and analyzed by using PCR with specific primer pair (FoxH1-BS, PCR cycle 40: Forward 5′-AGGATCTAGGGATCTCATTAGCAC-3′, Reverse 5′-AATCTGCTGTGACTGAAAGAAACTT-3′).
Tanaka K., Kato A., Angelocci C., Watanabe M, & Kato Y. (2014). A potential molecular pathogenesis of cardiac/laterality defects in Oculo-Facio-Cardio-Dental syndrome. Developmental biology, 387(1), 28-36.
+ Open protocol
+ Expand
7

Synthetic Lipids and Antibodies for Biomolecular Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following synthetic lipids were purchased from Avanti Polar Lipids: 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (POPC), 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphoethanolamine (POPE), 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphoserine (POPS), 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphoinositol (POPI), cholesterol, and the detergent n-Dodecyl-N,N-Dimethylamine-N-Oxide (LDAO) was from Anatrace. The following antibodies were used in this study: αHA (Santa Cruz, sc-7392, 1:200 dilution,), αMyc (Santa Cruz, sc-789 and sc-40, 1:1,000 dilution), αUb (mono- and poly-ubiquitinylated proteins, Enzo, BML-PW8810, 1:500 dilution), αPgk1 (Invitrogen, 459250, 1:1,000 dilution), and αGFP (Roche, 11814460001, 1:1000 dilution).
Matscheko N., Mayrhofer P., Rao Y., Beier V, & Wollert T. (2019). Atg11 tethers Atg9 vesicles to initiate selective autophagy. PLoS Biology, 17(7), e3000377.
+ Open protocol
+ Expand
8

Synthetic Lipids and Antibody Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Synthetic lipids 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (POPC), 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphoethanolamine (POPE), 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphoserine (POPS), 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphoinositol (POPI), cholesterol, 1,2-dioleoyl-sn-glycero-3-phospho-1′-myo-inositol-3′-phosphate (PI(3)-phosphate) as well as yeast polar lipid (YPL) extracts were purchased from Avanti Polar Lipids. The detergent n-Dodecyl-N,N-Dimethylamine-N-Oxide (LDAO) was from Anatrace.
The following antibodies were used in this study: αPep12p (Invitrogen, Catalog No: 710037, 1:1,000 dilution), αPgk1p (Invitrogen, Catalog No: 459250, 1:10,000 dilution), αHA (Santa Cruz, sc-7392, 1:200 dilution,), and αMyc (Santa Cruz, sc-789 and sc-40, 1:1,000 dilution), and αGFP antibodies (Roche, Catalog No: 11814460001).
Rao Y., Perna M.G., Hofmann B., Beier V, & Wollert T. (2016). The Atg1–kinase complex tethers Atg9-vesicles to initiate autophagy. Nature Communications, 7, 10338.
+ Open protocol
+ Expand
9

Antibody Characterization and Purification

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies against (α-) Piccolo (rabbit), Bassoon (mouse), and MAP2 (rabbit and mouse) were used as previously described [41 (link)]. α-Tubulin (mouse) antibodies were from Sigma, and α-PSD-95 (mouse) was from Affinity BioReagents. The following antibodies were purchased from Santa Cruz: α-Synaptophysin (rabbit), α-Trio (C-terminal antibody; goat), and α-Myc (rabbit). The mouse Trio α-GEF2 antibody was from Abnova. Mouse α-Synaptotagmin was purchased from BD Biosciences. The rabbit α-GFP antibody was from Invitrogen. The α-ELKS2 antibody was generated in rabbits using a commercial vendor (Washington Biotechnology). The epitope was E. coli purified GST-tagged amino acids 107–138 of ELKS2 [same region as used by [16 (link)]]. The serum was passed over a column of GST coupled to Actigel ALD using manufacturer’s protocol (Sterogene Bioseparations) to remove antibodies directed against GST. Antibody was then affinity purified with the antigen coupled to Actigel ALD.
Terry-Lorenzo R.T., Torres V.I., Wagh D., Galaz J., Swanson S.K., Florens L., Washburn M.P., Waites C.L., Gundelfinger E.D., Reimer R.J, & Garner C.C. (2016). Trio, a Rho Family GEF, Interacts with the Presynaptic Active Zone Proteins Piccolo and Bassoon. PLoS ONE, 11(12), e0167535.
+ Open protocol
+ Expand
10

Inducible Protein Expression in E. coli

Check if the same lab product or an alternative is used in the 5 most similar protocols

Escherichia coli MG1655 cells carrying arabinose‐inducible expression plasmids pBAD or pPER5 were grown overnight at 28°C in 3 ml of LB supplemented with appropriate antibiotics and 0.4% (wt./vol.) glucose. Cultures were normalized to OD600 = 0.1 and grown for 2 h at 28°C. Cells were then washed with LB to remove residual glucose, and 0.1% (wt./vol.) l‐arabinose was added to the media to induce protein expression. Cultures were grown for 2 or 4 h at 28°C as necessary, and 0.25 OD600 units of cells were pelleted and resuspended in 40 μl (2×) Tris‐Glycine sodium dodecyl sulfate (SDS) sample buffer (Novex, Life Sciences) supplemented with 0.05% (wt./vol.) β‐mercaptoethanol. Samples were boiled and cell lysates were fractionated by SDS‐PAGE, transferred onto PDVF membranes, and immunoblotted with α‐Myc (Santa Cruz Biotechnology, 9E10, mouse mAb) antibodies, used at 1:1000 dilution.
Carobbi A., Di Nepi S., Fridman C.M., Dar Y., Ben‐Yaakov R., Barash I., Salomon D, & Sessa G. (2022). An antibacterial T6SS in Pantoea agglomerans pv. betae delivers a lysozyme‐like effector to antagonize competitors. Environmental Microbiology, 24(10), 4787-4802.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!