Xf24 v28 cell culture microplate

Manufactured by Agilent Technologies

The XF24 V28 cell culture microplate is a laboratory equipment product designed for cellular analysis. It provides a standardized platform for conducting cell-based assays in a 24-well format. The microplate is intended for use with Agilent's XF Extracellular Flux Analyzers to measure cellular metabolism and function.

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Lab products found in correlation

Xf24 extracellular flux analyzer, by Agilent Technologies (2 mentions) Xf24 extracellular flux analyser, by Agilent Technologies (1 mentions) Agilent seahorse xf cell mito stress test kit, by Agilent Technologies (1 mentions) Mitotracker deep red fm, by Thermo Fisher Scientific (1 mentions) Cytexpert, by Beckman Coulter (1 mentions)

Spelling variants of this product

XF24 cell culture microplates (111 citations) XF24 microplate (14 citations) Cell culture microplate (12 citations) XF24 V28 microplate (5 citations) XF24 culture microplates (5 citations)

3 protocols using xf24 v28 cell culture microplate

Measuring Cellular Oxygen Consumption

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Oxygen consumption was measured using the XF24 Extracellular Flux Analyser from Seahorse Bioscience. For this, 2 h before the analysis, control, shTbx15 and Tbx15 overexpressing C2C12 myoblasts on 24-well XF24 V28 cell culture microplate (Seahorse Bioscience) were pretreated compound C. One hour before the experiment, cells were washed and incubated in 630 μl of non-buffered (without sodium carbonate) DMEM (4.5 g l⁻¹ glucose) pH 7.4 at 37 °C in a non-CO₂ incubator. Five replicates per cell type were included in the experiment, and four wells evenly distributed within the plate were used for correction of temperature variations. During the time course of the experiment, oxygen concentration was measured over time periods of 2 min at 6-min intervals, consisting of a 2 min of mixing period and a 4 min waiting period. OCR over the 2 min measurement period was calculated using the Fixed Delta technique for determining the slope.

Lee K.Y., Singh M.K., Ussar S., Wetzel P., Hirshman M.F., Goodyear L.J., Kispert A, & Kahn C.R. (2015). Tbx15 controls skeletal muscle fibre-type determination and muscle metabolism. Nature Communications, 6, 8054.

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Measuring Cellular Oxygen Consumption

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Oxygen consumption was measured using the XF24 Extracellular Flux Analyzer from Seahorse Bioscience. For this, 2 h prior to the analysis, control, shTbx15, and Tbx15 over-expressing C2C12 myoblasts on 24-well XF24 V28 cell culture microplate (Seahorse Bioscience) were pretreated Compound C. One hour before the experiment, cells were washed and incubated in 630 μl of non-buffered (without sodium carbonate) DMEM (4.5 g/L glucose) pH 7.4 at 37°C in a non-CO₂ incubator. 5 replicates per cell type were included in the experiment, and four wells evenly distributed within the plate were used for correction of temperature variations. During the time course of the experiment, oxygen concentration was measured over time periods of 2 min at 6 min intervals, consisting of a 2 min of mixing period and a 4 min waiting period. Oxygen consumption rate (OCR) over the 2 min measurement period was calculated using the Fixed Delta technique for determining the slope.

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Mitochondrial Activity Analysis in Brown Adipocytes

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MitoTracker Deep Red FM (Invitrogen, M22426) was used to stain active respiring mitochondria. Brown adipocytes subjected to different treatments were incubated with 25 nM MitoTracker Deep Red FM in the dark at 37 °C in 5% CO₂ for 30 minutes. Cells were then digested by trypsin and washed in PBS. The samples were analyzed immediately by flow cytometry, and data were further processed by CytExpert (Beckman).
For oxygen consumption analysis, cells were plated in the XF24 V28 cell culture microplate (Seahorse Bioscience) and subjected to adipocyte differentiation for 7 days. Then cells were treated with BML-260 (10 μM) or DMSO as control for 3 days before measuring the oxygen consumption rate (OCR) using an XF24 Extracellular Flux Analyzer (Seahorse Bioscience). Oligomycin (2 μM), FCCP (1.5 μM) and Rotenone/Antimycin A (1 μM) from the Agilent Seahorse XF Cell Mito Stress Test kit (Agilent Technologies, 103015-100) were added during fixed time intervals to modulate respiration. The OCR values were normalized by total protein levels in each-well for calculation.

Feng Z., Wei Y., Zhang Y., Qiu Y., Liu X., Su L., Liang N., Yin H, & Ding Q. (2019). Identification of a rhodanine derivative BML-260 as a potent stimulator of UCP1 expression. Theranostics, 9(12), 3501-3514.

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