Pe conjugated active caspase 3 apoptosis kit

Manufactured by BD

The PE-conjugated active caspase-3 apoptosis kit is a laboratory tool used to detect and measure the presence of activated caspase-3, a key enzyme involved in the apoptosis (programmed cell death) process. The kit utilizes a PE (phycoerythrin) fluorescent dye that binds to the active form of caspase-3, allowing for its quantification and analysis.

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Lab products found in correlation

Topro 3 or dapi, by Thermo Fisher Scientific (1 mentions) Fortessa x20 flow cytometer, by BD (1 mentions) Easylyse, by Agilent Technologies (1 mentions) Kaluza 2, by Beckman Coulter (1 mentions)

Close spelling variants of this product

PE-conjugated monoclonal active-caspase-3 antibody apoptosis kit PE Active Caspase-3 Apoptosis Kit Active Caspase-3 Apoptosis Kit Active caspase-3 Caspase-3 Apoptosis kits

2 protocols using pe conjugated active caspase 3 apoptosis kit

Multicolor Flow Cytometry Analysis

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FITC-conjugated monoclonal antibodies (mAbs) to CD14, CD19, CD20, and CD66b; PE-conjugated mAbs to CD19, CD20, CD54, and CD105; PC7-conjugated mAbs to CD19, and CD20; and PB-conjugated mAbs to CD15 were provided by Beckman Coulter. Alexa-647-cojugated mAb to CD105 was provided by ebioscience. Appropriate isotype-matched mAbs were used as negative controls and all analyses were performed using a Gallios (Beckman Coulter) flow cytometer. Cell death was checked using TOPRO-3 or DAPI (Life Technologies) staining. Apoptosis evaluation was performed using PE-conjugated active caspase-3 apoptosis kit (Becton Dickinson).

Grégoire M., Guilloton F., Pangault C., Mourcin F., Sok P., Latour M., Amé-Thomas P., Flecher E., Fest T, & Tarte K. (2015). Neutrophils trigger a NF-κB dependent polarization of tumor-supportive stromal cells in germinal center B-cell lymphomas. Oncotarget, 6(18), 16471-16487.

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Apoptotic T Cell Quantification

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Whole blood was incubated with CD3 BV421 (Becton Dickinson). Erythrocytes were then lysed twice using Easylyse (Dako, Glostrup, Denmark). To evaluate the proportion of apoptotic T cells, PE-conjugated active caspase-3 apoptosis kit (Becton Dickinson) has been used. Samples were run in a Fortessa X20 flow cytometer (Becton Dickinson) and data were analyzed using Kaluza 2.0 software (Beckman Coulter).

Reizine F., Lesouhaitier M., Gregoire M., Pinceaux K., Gacouin A., Maamar A., Painvin B., Camus C., Le Tulzo Y., Tattevin P., Revest M., Le Bot A., Ballerie A., Cador-Rousseau B., Lederlin M., Lebouvier T., Launey Y., Latour M., Verdy C., Rossille D., Le Gallou S., Dulong J., Moreau C., Bendavid C., Roussel M., Cogne M., Tarte K, & Tadié J.M. (2021). SARS-CoV-2-Induced ARDS Associates with MDSC Expansion, Lymphocyte Dysfunction, and Arginine Shortage. Journal of Clinical Immunology, 41(3), 515-525.

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